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Exploration for the impacts of halosaline circulation on microbial geography

Research Project

Project/Area Number 26550023
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental dynamic analysis
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

NUNOURA Takuro  国立研究開発法人海洋研究開発機構, 海洋生命理工学研究開発センター, 研究開発センター長代理 (60359164)

Co-Investigator(Kenkyū-buntansha) 高木 善弘  国立研究開発法人海洋研究開発機構, 深海・地殻内生物圏研究分野, 主任技術研究員 (10399561)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords海洋微生物 / 熱塩循環 / 海洋循環 / メタゲノム / 微生物生態 / 多様性
Outline of Final Research Achievements

To know the relationship between microbial distribution and thermohaline circulation, we conducted high-resolution microbial sampling and ecological analyses on an observatory transect across north Pacific subarctic zone. Moreover, we tried to construct a method of shotgun metaegnomic library construction from less than 1 ng of DNA to apply the genome level identification of the deep waters along with the observatory transect. As a result, we found anomalous increasing of microbial biomass 1000-2000 m above the seafloor that are not simply explained by the effects of sinking organic matters. We conclude that the thermohaline circulation likely influenced the microbial ecosystem in deep waters. In addition, we successfully constructed shotgun metagenomic libraries from 10 pg of DNA. We are processing molecular data sets to clarify the relationship between microbial distribution and thermohaline circulation.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (1 results)

All 2016

All Presentation (1 results)

  • [Presentation] 超微量環境DNAからのメタゲノムライブラリー構築に関する技術的検討2016

    • Author(s)
      平井美穂、西真郎、津田美和子、高木善弘、布浦拓郎
    • Organizer
      日本微生物生態学会第31回大会
    • Place of Presentation
      横須賀市文化会館(神奈川県横須賀市)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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