Evaluation of biological effects induced by ionizing radiation and stress using DNA damage foci formation visualized in living hippocampus neuron and retinal ganglion cells.
| Project/Area Number |
26550029
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Tohoku Medical and Pharmaceutical University (2016-2017)
Tottori University (2014-2015) |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | DNA損傷 / 放射線 / DNA損傷応答 / 53BP1 / 海馬 / 神経細胞 / タイムラプス / 網膜 / 海馬神経細胞 / 生細胞 / 組織 / フォーカス形成 / 神経 / DNA修復 / DNA損傷フォーカス / 網膜神経節細胞 |
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| Outline of Final Research Achievements |
Ionizing radiation and inside or outside stress induced in our body result in a increased ROS production and a induction of DNA double-strand breaks in the cells. A hypoxia caused by cerebral ischemia or glaucoma, nuclear foci formation by DNA double-strand breaks is also reported. A sensor of DNA damage foci formation using EGFP-53BP1 fusion protein makes DNA damage formation visible in the living mouse neuronal tissues or cells. As a result of our research, there is a clear difference of foci formation between differentiated tissue in vivo and culture cells in vitro, suggesting there exist special characteristics of foci formation and DNA damage response in the differentiated tissue cells.
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Report
(5 results)
Research Products
(12 results)
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[Journal Article] Differential Radiosensitivity Phenotypes of DNA-PKcs Mutations Affecting NHEJ and HRR Systems following Irradiation with Gamma-Rays or Very Low Fluences of Alpha Particles.2014
Author(s)
Lin YF, Nagasawa H, Little JB, Kato TA, Shih HY, Xie XJ, Wilson PF Jr, Brogan JR, Kurimasa A, Chen DJ, Bedford JS, Chen BP.
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Journal Title
PLoS One.
Volume: 9(4)
Issue: 4
Pages: e93579-e93579
DOI
Related Report
Peer Reviewed / Open Access
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