| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
DNA-protein cross-links (DPCs) are major DNA lesions induced by ionizing radiation and anticancer drugs. However, it remains elusive whether some regions of the genome are more vulnerable with respect to DPC formation than others when cells are exposed to DNA damaging agents. In the present study, we developed a method for genomic mapping of radiation-induced DPCs. Mouse SCCVII tumors were irradiated with X-rays and DNA was purified. Proteins present in DPCs were labeled with biotin and DNA fragments containing DPCs (DPC-DNA) were specifically pulled down using avidin-beads. A synthetic adaptor was ligated at both ends of DPC-DNA. The resulting DNA was subjected to PCR. Analysis of reaction products with agarose gel electrophoresis showed that DPC-DNA was successfully amplified by PCR.
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