• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A mechanism for the reduced DNA damage in mice irradiated in fetuses and newborns

Research Project

Project/Area Number 26550032
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Risk sciences of radiation and chemicals
Research InstitutionOsaka Prefecture University

Principal Investigator

KODAMA Seiji  大阪府立大学, 理学(系)研究科(研究院), 教授 (00195744)

Research Collaborator SAKAGUCHI Kenta  大阪府立大学, 理学系研究科生物科学専攻, 博士後期課程3年
KASHIWAGI Hiroki  大阪府立大学, 理学系研究科生物科学専攻, 博士後期課程3年
SHIRASAKA Yosuke  大阪府立大学, 理学系研究科生物科学専攻, 博士前期課程2年
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords放射線損傷 / 胎内被ばく / DNA2本鎖切断 / 染色体転座 / 神経幹/前駆細胞 / 損傷 / DNA損傷 / 染色体異常
Outline of Final Research Achievements

Fetal irradiation of humans and mice do not induce persistent chromosome translocations in lymphocytes similar to the level after adult irradiation. In the present study, we scored DNA double strand breaks and chromosome translocations in neural stem/progenitor cells of mice at 6 weeks of age and their mothers after they were exposed to 2 Gy of whole-body X-irradiation when the gestation period was 14.5 days. The results indicated that X-ray-induced DNA double strand breaks were not persistent in neural stem/progenitor cells of both offspring and their mothers, resulting in no difference between them. In contrast, the frequency of X-ray-induced chromosome translocations in offspring was significantly lower (p=0.0247, Chi-square with Yates’ correction) than that of mothers in neural stem/progenitor cells in addition to lymphocytes.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (17 results)

All 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (13 results) (of which Int'l Joint Research: 5 results) Remarks (1 results)

  • [Journal Article] Promoted instability in an X-ray irradiated chromosome transferred into Werner syndrome cells.2015

    • Author(s)
      Ariyoshi, K., Suzuki, K., Watanabe, M., and Kodama, S.
    • Journal Title

      Radiation Emergency Medicine

      Volume: 4 Pages: 40-46

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chromosome loss caused by DNA fragmentation induced in main nuclei and micronuclei of human lymphoblastoid cells treated with colcemid2014

    • Author(s)
      Yamamoto, M., Wakata, A., Aoki, Y., Miyamae, Y., and Kodama, S.
    • Journal Title

      Mutat. Res .

      Volume: 762 Pages: 10-16

    • DOI

      10.1016/j.mrfmmm.2014.02.002

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Induction of genetic instability by transfer of a UV-A-irradiated chromosome2014

    • Author(s)
      Urushibara, A., Kodama, S., and Yokoya, A.
    • Journal Title

      Mutat. Res

      Volume: 766 Pages: 29-34

    • DOI

      10.1016/j.mrgentox.2014.02.005

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 胎内被ばくマウスにおけるDNA損傷の解析2015

    • Author(s)
      白阪耀介、坂口健太、白石一乗、児玉靖司
    • Organizer
      日本放射線影響学会第1回放射線ワークショップ
    • Place of Presentation
      富山大学黒田講堂(富山県富山市)
    • Year and Date
      2015-10-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] G1期及びS/G2期に移入されたX線被ばく染色体の安定性の解析2015

    • Author(s)
      西田拓馬、白石一乗、児玉靖司
    • Organizer
      日本放射線影響学会第1回放射線ワークショップ
    • Place of Presentation
      富山大学黒田講堂(富山県富山市)
    • Year and Date
      2015-10-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] マウス神経幹/前駆細胞におけるX線誘発DNA 損傷の新しい検出系開発2015

    • Author(s)
      山田聖、白石一乗、児玉靖司
    • Organizer
      日本放射線影響学会第1回放射線ワークショップ
    • Place of Presentation
      富山大学黒田講堂(富山県富山市)
    • Year and Date
      2015-10-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] Establishment and radio-biological characterization of immortal mouse neural stem cell lines.2015

    • Author(s)
      Seiji Kodama, Tomoya Nakahama, Hiroki Kashiwagi, Kae Imanishi, Takayo Awatani, Kazunori Shiraishi
    • Organizer
      15th International Congress of Radiation Research
    • Place of Presentation
      Kyoto International Conference Center, Kyoto, Japan
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Repair of DNA double strand breaks induced by X-irradiation in mouse neurons.2015

    • Author(s)
      Hiroki Kashiwagi, Tomoya Nakahama, Yosuke Shirasaka, Kenta Sakaguchi, Kazunori Shiraishi, Seiji Kodama
    • Organizer
      15th International Congress of Radiation Research
    • Place of Presentation
      Kyoto International Conference Center, Kyoto, Japan
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of an image-J based program for counting γ-H2AX foci in mouse neurosphere cells.2015

    • Author(s)
      Kenta Sakaguchi, Hiroki Kashiwagi, Yosuke Shirasaka, Maki Fukui, Kazunori Shiraishi, Seiji Kodama
    • Organizer
      15th International Congress of Radiation Research
    • Place of Presentation
      Kyoto International Conference Center, Kyoto, Japan
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] X-ray-induced chromosome segregation errors analyzed by live cell imaging.2015

    • Author(s)
      Shohei Okamoto, Ai Kawakita, Kori Murata, Kazunori Shiraishi, Kenji Sugimoto, Seiji Kodama
    • Organizer
      15th International Congress of Radiation Research
    • Place of Presentation
      Kyoto International Conference Center, Kyoto, Japan
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Chromosomal instability transmitted by a microcell exposed to X-rays.2015

    • Author(s)
      Yuki Hirakawa, Masateru Tanabe, Takuma Nishida, Kazunori Shiraishi, Seiji Kodama
    • Organizer
      15th International Congress of Radiation Research
    • Place of Presentation
      Kyoto International Conference Center, Kyoto, Japan
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] p53 dependent timing of DNA synthesis in main nuclei and micronuclei2014

    • Author(s)
      Seiji Kodama, Kohei Hagihara, Kazunori Shiraishi, Ai Kawakita, Kaori Murata, Kenji Sugimoto
    • Organizer
      日本放射線影響学会第57回大会
    • Place of Presentation
      かごしま県民交流センター(鹿児島)
    • Year and Date
      2014-10-01 – 2014-10-03
    • Related Report
      2014 Research-status Report
  • [Presentation] Chromosome instability transmitted by a microcell exposed to ionizing radiation2014

    • Author(s)
      Yuki Hirakawa, Kazunori Shiraishi, Seiji Kodama
    • Organizer
      日本放射線影響学会第57回大会
    • Place of Presentation
      かごしま県民交流センター(鹿児島)
    • Year and Date
      2014-10-01 – 2014-10-03
    • Related Report
      2014 Research-status Report
  • [Presentation] X-ray-induced chromosome bridges analyzed by live cell imaging2014

    • Author(s)
      Shohei Okamoto, Ai Kawakita, Kaori Murata, Kazunori Shiraishi, Kenji Sugimoto, Seiji Kodama
    • Organizer
      日本放射線影響学会第57回大会
    • Place of Presentation
      かごしま県民交流センター(鹿児島)
    • Year and Date
      2014-10-01 – 2014-10-03
    • Related Report
      2014 Research-status Report
  • [Presentation] DNA double-strand break repair in mouse neurons2014

    • Author(s)
      Hiroki Kashiwagi, Tomoya Nakahama, Kenta Sakaguchi, Kazunori Shiraishi, Seiji Kodama
    • Organizer
      日本放射線影響学会第57回大会
    • Place of Presentation
      かごしま県民交流センター(鹿児島)
    • Year and Date
      2014-10-01 – 2014-10-03
    • Related Report
      2014 Research-status Report
  • [Presentation] Establishment and rdio-biological characterization of immortal mouse neural stem cell lines2014

    • Author(s)
      Tomoya Nakahama, Hiroki Kashiwagi, Kazunori Shiraishi, Seiji Kodama
    • Organizer
      日本放射線影響学会第57回大会
    • Place of Presentation
      かごしま県民交流センター(鹿児島)
    • Year and Date
      2014-10-01 – 2014-10-03
    • Related Report
      2014 Research-status Report
  • [Remarks] 大阪府立大学大学院理学系研究科生物科学専攻放射線生物学研究室

    • URL

      http://chokai.riast.osakafu-u.ac.jp/%7Ehousya6/home.html

    • Related Report
      2015 Annual Research Report 2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi