| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
In this study, in order to gain insight into the unknown mechanism of cluster DNA damage-induced mutagenesis, we investigated whether DNA strand with mutagenic DNA damage is preferentially replicated by DNA polymerase(s). We developed a new method for constructing plasmids that can determine the fraction of DNA strand used as the template for replication in plasmids carrying cluster DNA damage. Using plasmids constructed by the method developed in this study, we found that (1) one of the strand is preferentially replicated around cluster DNA damage, and (2) several tens of base pairs in length are preferentially replicated, and (3) the preferential replication is mediated by PolI.
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