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Novel mutagenic pathway involving DNA polymesase

Research Project

Project/Area Number 26550034
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Risk sciences of radiation and chemicals
Research InstitutionNational Institutes for Quantum and Radiological Science and Technology (2016-2017)
Japan Atomic Energy Agency (2014-2015)

Principal Investigator

Shikazono Naoya  国立研究開発法人量子科学技術研究開発機構, 関西光科学研究所 量子生命科学研究部, 部長(定常) (10354961)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsクラスターDNA損傷 / 変異メカニズム / 突然変異 / 突然変異誘発機構 / DNA基質
Outline of Final Research Achievements

In this study, in order to gain insight into the unknown mechanism of cluster DNA damage-induced mutagenesis, we investigated whether DNA strand with mutagenic DNA damage is preferentially replicated by DNA polymerase(s). We developed a new method for constructing plasmids that can determine the fraction of DNA strand used as the template for replication in plasmids carrying cluster DNA damage. Using plasmids constructed by the method developed in this study, we found that (1) one of the strand is preferentially replicated around cluster DNA damage, and (2) several tens of base pairs in length are preferentially replicated, and (3) the preferential replication is mediated by PolI.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (16 results)

All 2017 2016 2015 2014

All Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Acknowledgement Compliant: 4 results) Presentation (10 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results)

  • [Journal Article] Radiation-induced clustered DNA lesions: repair and mutagenesis2017

    • Author(s)
      E. Sage and N. Shikazono
    • Journal Title

      Free Rad. Med. Biol.

      Volume: 107 Pages: 125-135

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] New method for estimating clustering of DNA lesions induced by physical/chemical mutagens using fluorescence anisotropy2017

    • Author(s)
      K. Akamatsu, N. Shikazono, and T. Saito
    • Journal Title

      Analytical Biochemistry

      Volume: 536 Pages: 78-89

    • DOI

      10.1016/j.ab.2017.08.007

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Radiation-induced clustered DNA lesions: Repair and mutagenesis2017

    • Author(s)
      E. Sage and N. Shikazono
    • Journal Title

      Free Radical Biology and Medicine

      Volume: 印刷中 Pages: 125-135

    • DOI

      10.1016/j.freeradbiomed.2016.12.008

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Efficiency of radiation-induced base lesion excision and the order of enzymatic treatment2016

    • Author(s)
      Shiraishi, I., Shikazono, N., Fujii, K. and Yokoya, A.
    • Journal Title

      Int. J. Radiat. Biol.

      Volume: 93 Issue: 3 Pages: 295-302

    • DOI

      10.1080/09553002.2017.1239849

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 放射線生物影響の原因となるDNA損傷の塊2015

    • Author(s)
      鹿園直哉
    • Journal Title

      Isotope News

      Volume: 738 Pages: 6-10

    • Related Report
      2015 Research-status Report
    • Acknowledgement Compliant
  • [Journal Article] Localization estimation of ionizing radiation-induced abasic sites in DNA in the solid state using fluorescence resonance energy transfer.2015

    • Author(s)
      Akamatsu K, Shikazono N, Saito T
    • Journal Title

      Radiation Research

      Volume: 183 Issue: 1 Pages: 105-113

    • DOI

      10.1667/rr13780.1

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Detection and mutagenic potential of clustered DNA lesions.2017

    • Author(s)
      N. Shikazono, K. Akamatsu
    • Organizer
      17th International Symposium on Microdosimetry
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 電離放射線によって生じたDNA損傷の局在性について フェルスター共鳴エネルギー移動(FRET)を用いたアプローチ2017

    • Author(s)
      赤松憲、鹿園直哉
    • Organizer
      第60回日本放射線影響学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 各種DNA損傷因子によって生じたクラスターDNA損傷のキャラクタリゼーション2017

    • Author(s)
      赤松憲、鹿園直哉
    • Organizer
      第60回日本放射線影響学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] クラスターDNA損傷及びミスマッチを有するプラスミドの作製法の開発2016

    • Author(s)
      高橋桃子、赤松憲、鹿園直哉
    • Organizer
      日本放射線影響学会
    • Place of Presentation
      JMSアステールプラザ(広島市、広島県)
    • Year and Date
      2016-10-26
    • Related Report
      2016 Research-status Report
  • [Presentation] 蛍光異方性を利用した放射線DNA損傷の局在性評価法の開発―細胞核内クラスター損傷の観察に向けた基礎検討―2016

    • Author(s)
      赤松憲、鹿園直哉
    • Organizer
      日本放射線影響学会
    • Place of Presentation
      JMSアステールプラザ(広島市、広島県)
    • Year and Date
      2016-10-26
    • Related Report
      2016 Research-status Report
  • [Presentation] Using laser-driven ion sources to study fast radiobiological processes2015

    • Author(s)
      N.Shikazono
    • Organizer
      Application of laser-driven ion particles
    • Place of Presentation
      Venice International University(Venice, Italy)
    • Year and Date
      2015-11-19
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Biological consequences of clustered DNA damage in Escherichia coli2015

    • Author(s)
      N. Shikazono and K. Akamatsu
    • Organizer
      15th International Congress of Radiation Research
    • Place of Presentation
      京都国際会館(京都府京都市)
    • Year and Date
      2015-05-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Localization estimation of ionizing radiation- induced abasic sites in DNA using FRET2015

    • Author(s)
      K. Akamatsu and N. Shikazono
    • Organizer
      15th International Congress of Radiation Research
    • Place of Presentation
      京都国際会館(京都府京都市)
    • Year and Date
      2015-05-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] クラスターDNA損傷による変異誘発におけるDNAポリメラーゼの関与2014

    • Author(s)
      鹿園直哉、赤松憲
    • Organizer
      第57回日本放射線影響学会
    • Place of Presentation
      かごしま県民交流センター(鹿児島県鹿児島市)
    • Year and Date
      2014-10-01 – 2014-10-03
    • Related Report
      2014 Research-status Report
  • [Presentation] FRETを用いた放射線DNA損傷の局在性評価2014

    • Author(s)
      赤松憲、鹿園直哉
    • Organizer
      第57回日本放射線影響学会
    • Place of Presentation
      かごしま県民交流センター(鹿児島県鹿児島市)
    • Year and Date
      2014-10-01 – 2014-10-03
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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