| Project/Area Number |
26550041
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
YAGI TAKASHI 大阪府立大学, 理学(系)研究科(研究院), 教授 (80182301)
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| Co-Investigator(Kenkyū-buntansha) |
KAWANISHI Masanobu 大阪府立大学, 理学系研究科, 准教授 (70332963)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | エピジェネティックス / エピミュータジェン / バイオアッセイ / ５-メチルシトシン / プロモーター |
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| Outline of Final Research Achievements |
Aberrant epigenetic alteration of genes, that is, cytosine methylation and demethylation, is caused occasionally when the cells are exposed to particular exogenous chemicals. We found that O6-methylguanine-DNA-methyltransferase (MGMT) gene promoter is the suitable DNA sequence that can detect the epigenetic alteration. In this study, we made the reporter plasmid that has hypoxanthine-phosphoribosyltransferase (hprt) cDNA under the control of the MGMT promoter and also has neomycin-resistance gene. We introduced the plasmid into hprt-deficient cells and established the cell line that becomes 6-thioguanine resistance by inactivation of the introduced hprt by the promoter methylation. We are now exploring the epimutagenic activity in a large variety of man-made substances.
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