| Project/Area Number |
26550046
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental impact assessment
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Sakai Yasuyuki 東京大学, 大学院工学系研究科(工学部), 教授 (00235128)
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| Co-Investigator(Kenkyū-buntansha) |
諏訪部 章 岩手医科大学, 医学部, 教授 (20241713)
岩沢 こころ 東京大学, 生産技術研究所, 特任研究員 (30402796)
小笠原 理恵 岩手医科大学, 医学部, 助教 (70347871)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 細胞・組織 / 生物・生体工学 / 環境 / 薬学 / 培養モデル / ナノ粒子 / 肺吸収 / 経肺吸収 / 肺胞 / 上皮細胞 / マクロファージ / 面密度 |
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| Outline of Final Research Achievements |
Three in vitro pulmonary alveolus culture models were established. Our aim was to optimize existing human lung in vitro models to better mimic the in vivo situation to study the nanoparticles toxicity and translocation study. Epithelial and endothelial cells were separately cultured on the opposite sides of the cell culture insert consisting of the polycarbonate microporous membrane to form separate cell layers. This co-culture system is proved to be a novel pulmonary alveolus model that might be applied to evaluate the safety evaluation of drugs, chemicals and nanomaterials. Then primary epithelial cells were used in the second rat alveolar tissue model, which could be used to directly compare in vivo and in vitro conditions. Results likely indicate the formation of the rat primary culture model with high stability. This primary culture system is a novel pulmonary alveolus model and might be applied to evaluate the safety evaluation of drugs, chemicals and nanomaterials.
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