| Project/Area Number |
26550057
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental engineering and reduction of environmental burden
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
熊野 匠人 筑波大学, 生命環境系, 助教 (70585025)
小林 達彦 筑波大学, 生命環境系, 教授 (70221976)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 微生物 / 酵素 / 精製 / 廃棄物処理 / 応用微生物学 |
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| Outline of Final Research Achievements |
The azide compounds composed of three nitrogen atoms joined together by a double bond (R-N=N=N), are known to be extremely toxic and explosive. However, metabolic pathways of azide compounds have been unclear. We isolated a microorganism that degrades an azide compound from soil and discovered a novel enzyme involved in the metabolism of the azide compound.
Through several steps from a cell-free extract of the strain cultured under the optimum culture condition, we purified the azide compound-degrading enzyme to homogeneity. Some properties of this enzyme including substrare specificities, optimum pH and pH stability, were determined. Based on the N-terminal amino acid sequences of the purified enzyme, the structure gene coding for the enzyme was cloned.
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