| Project/Area Number |
26550065
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental engineering and reduction of environmental burden
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
Nomura Toshiyuki 大阪府立大学, 工学(系)研究科(研究院), 准教授 (00285305)
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| Co-Investigator(Kenkyū-buntansha) |
TANI SHUJI 大阪府立大学, 生命環境科学研究科, 講師 (80405357)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ナノ粒子 / 酵母 / エンドサイトーシス |
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| Outline of Final Research Achievements |
We examined the introduction of nanoparticles into cells equipped with a rigid cell wall. Yeast cells were used as model microbial cells, and the positively charged polystyrene latex (PSL) nanoparticles were used as model nanoparticles. As a result, we successfully demonstrated that the introduction of the PSL nanoparticles into the living yeast cells could be promoted using anionic carboxymethylcellulose. In addition, the RNA-Seq analysis of the yeast cells which took in the PSL nanoparticles were performed. As a result, it was suggested that the cells were subjected to stress from the nanoparticles.
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