| Project/Area Number |
26550085
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Environmental risk control and evaluation
|
|---|
| Research Institution | Fisheries Research and Education Agency |
|---|
Principal Investigator |
Nagai Satoshi 国立研究開発法人水産研究・教育機構, 中央水産研究所, グループ長 (80371962)
|
|---|
| Research Collaborator |
MIYAMOTO Nobuhiko 株式会社カネカ, 研究部探索グループ, 研究主任
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | 核酸クロマト / multiplex-PCR / 有毒渦鞭毛藻 / 有害赤潮 / 分子同定 / PCR増幅 / Alexandrium / 核酸クロマトグラフィー / multiplex PCR / maultiplex-PCR |
|---|
| Outline of Final Research Achievements |
In this study, the Kaneka DNA chromatography chip (KDCC) for Alexandrium species was successfully developed that enables the simultaneous detection of five Alexandrium species. This method is utilized a DNA-DNA hybridization technology. This technique is simple, because it is possible to detect the target species by naked eye soon after (< 5 min) applying two μL of PCR amplicon and 65 μL of development buffer to the sample pad of the chip. Further, this technique is relatively inexpensive (does not require expensive laboratory equipment such as real-time Q-PCR machines or DNA microarray detectors, but a thermal cycler). Regarding the detection limit of KDCC for the five Alexandrium species, it varied among species and they were <0.1-10 pg and equivalent to 5-500 copies of rRNA genes, indicating that the technique is sensitive enough for practical use to detect several cells of the target species from 1 L of seawater.
|
