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Development of a novel method for identifying a wide variety of species from environmental DNA

Research Project

Project/Area Number 26550089
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental and ecological symbiosis
Research InstitutionKyushu University

Principal Investigator

Sato Tetsuya  九州大学, 生体防御医学研究所, 助教 (00457425)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords生物多様性 / 環境DNA / 環境保全 / 次世代シーケンサー / ユニバーサルプライマー
Outline of Final Research Achievements

Environmental DNA (eDNA), most likely derived from faces and so on, is detected in lakes and rivers. Although the eDNA can be used to investigate the types of organisms that live in the environment, a conventional method using species-specific primer sequences can only be identified limited species. In this study, by designing original universal primers, we successfully developed a new method to identify a wide variety of living species in the environment. The results in this study would be expected to the biodiversity survey and assessment.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (5 results)

All 2015 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (1 results) Remarks (2 results)

  • [Journal Article] ChromContact: A web tool for analyzing spatial contact of chromosomes from Hi-C data.2015

    • Author(s)
      Sato T, Suyama M.
    • Journal Title

      BMC Genomics

      Volume: 16 Issue: 1 Pages: 1060-1060

    • DOI

      10.1186/s12864-015-2282-x

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] GenomeCons: a web server for manipulating multiple genome sequence alignments and their consensus sequences2015

    • Author(s)
      Sato T, Suyama M
    • Journal Title

      Bioinformatics

      Volume: 31 Issue: 8 Pages: 1293-1295

    • DOI

      10.1093/bioinformatics/btu803

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 環境DNAから多種多様な生物種を同定する新規手法2015

    • Author(s)
      佐藤哲也,大川恭行,須山幹太
    • Organizer
      日本生態学会第62回全国大会
    • Place of Presentation
      鹿児島大学(鹿児島県・鹿児島市)
    • Year and Date
      2015-03-21
    • Related Report
      2014 Research-status Report
  • [Remarks] ChromContactウェブサーバ

    • URL

      http://bioinfo.sls.kyushu-u.ac.jp/chromcontact/

    • Related Report
      2015 Annual Research Report
  • [Remarks] GenomeConsウェブサーバ

    • URL

      http://bioinfo.sls.kyushu-u.ac.jp/genomecons/

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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