Investigating the effectiveness of a new amniotic fluid test for evaluating the prenatal nutritional environment

• Project/Area Number: 26560063
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: The University of Shiga Prefecture
• Principal Investigator: Sano Mitsue 滋賀県立大学, 人間文化学部, 助教 (20524911)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 羊水 / 胎児 / 母体栄養 / DOHad / 分析 / 食事調査 / 妊娠 / ラット / 葉酸 / 羊水成分

Outline of Final Research Achievements

This study aimed to reveal the extent to which the nutritional environment of the mother during pregnancy influences that of the fetus. Focusing on amniotic fluid, two experiments were performed to obtain insight into the effects of maternal nutritional status on nutrient transport to the fetus: Experiment (1), involving pregnant rats fed a low-carbohydrate, high-fat diet (KD), and Experiment (2), involving an analysis of nutrient-derived components in the blood, urine ((1) only) and amniotic fluid of pregnant women (rats). Sample concentrations of glucose, ketone bodies, free amino acids ((2) only), Tryptophan ((1) only), NAD, folic acid, and vitamin B12 were measured. Pregnant women were administered a food survey in addition. In Experiment (1), ketone body levels in mother and fetuses and conversion ratio of Tryptophan to Niacin in mothers rose in KD group. This finding demonstrated that maternal ketotic state affects the fetus. Experiment (2) is still under investigation.

Research Products

• [Int'l Joint Research] INRA(フランス)
• [Journal Article] Maternal and foetal tryptophan and serotonin metabolism in gestating rat: effect of intrauterine growth restriction. (2016)
  • Author(s): Sano M, Ferchaud-Roucher V, Kaeffer B, Poupeau G, Castellano B & Darmaun D.
  • Journal Title: Amino Acids Volume: 48 Issue: 1 Pages: 281-290
  • DOI: 10.1007/s00726-015-2072-4
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] High fat and very low carbohydrate intake during pregnancy does not influence the folate nutritional status in mother and foetuses (2017)
  • Author(s): M. Sano, M. Suzuki, M. Funato
  • Organizer: DOHaD 2017
  • Place of Presentation: ロッテルダム（オランダ）
  • Year and Date: 2017-10-15
  • Int'l Joint Research
• [Presentation] 高脂肪低炭水化物食摂取による糖新生亢進が妊娠母体とその胎児へ及ぼす栄養学的影響 (2015)
  • Author(s): 佐野光枝，福渡努，柴田克己
  • Organizer: 第62回日本栄養改善学会総会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2015-09-24
• [Presentation] 安定同位体：15N2-L-トリプトファンを用いたin vivo トレーサー実験法の確立 (2014)
  • Author(s): 佐野光枝，Veronique Ferchaud-Roucher, Charlotte Nael, Audrey Aguesse, Guillaume Poupeau, Blandine Castellano and Dominique Darmaun
  • Organizer: 第3回日本DOHad研究会学術集会
  • Place of Presentation: 国立成育医療研究センター（東京）
  • Year and Date: 2014-07-25 – 2014-07-26
• [Presentation] 安定同位体：15N2-L-トリプトファンを用いたin vivo トレーサー実験法の確立 (2014)
  • Author(s): 佐野光枝，Veronique Ferchaud-Roucher, Charlotte Nael, Audrey Aguesse, Guillaume Poupeau, Blandine Castellano and Dominique Darmaun
  • Organizer: 第68回日本栄養・食糧学会大会
  • Place of Presentation: 酪農学園大学（北海道）
  • Year and Date: 2014-05-30 – 2014-06-01