The study on the bio-marker for fatigue, and the development of the functional food

• Project/Area Number: 26560070
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: Meijo University
• Principal Investigator: MINATO Ken-ichiro 名城大学, 農学部, 准教授 (10341728)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 食用きのこ / 炎症抑制作用 / 樹状細胞 / マクロファージ / 免疫調節作用 / βグルカン / 単球 / 食用キノコ / M1マクロファージ / 抗炎症作用 / サイトカイン / 抗疲労食品 / バイオマーカー / ミクログリア / 香辛料 / 抗炎症 / ホメオスタシス

Outline of Final Research Achievements

It has been shown that a fatigue could be caused by the inflammatory response of immune cells such as dendritic cells, macrophages and monocytes. In this study, the author has shown that both pro- and anti-inflammatory cytokines could become good bio-markers to diagnose fatigue. Moreover, some mushroom showed anti-inflammatory effect on the immune cells. And, the glucan from Pleurotus cornucopiae mushroom could suppress a differentiation of monocyte into inflamed macrophage despite the finding that the glucan induced to inflame matured dendritic cells, macrophages and monocytes. Therefore, this study has considered that some mushrooms can inhibit inflammation of immune cells, and prevent the fatigue.

Research Products

• [Int'l Joint Research] VU University Medical Center(オランダ)
• [Int'l Joint Research] VU University Medical Center(オランダ)
• [Journal Article] A pro-inflammatory effect of the β-glucan from Pleurotus cornucopiae mushroom on macrophage action (2017)
  • Author(s): K. Minato, A. Ohara, M. Mizuno
  • Journal Title: Mediators of Inflammation Volume: 印刷中
  • NAID: 120006358596
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Pleurotus citrinopileatus polysaccharide induces activation of human dendritic cells through multiple pathways (2016)
  • Author(s): K. Minato, L. Laan, A. Ohara, I. van Die
  • Journal Title: International Immunopharmacology Volume: 40 Pages: 156-163
  • DOI: 10.1016/j.intimp.2016.08.034
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Safety evaluation of Grifola gargal, an edible basidiomycete from Chile (2014)
  • Author(s): Harada, E., Morizono, T., Minato, K., Ohara, A., Hasegawa, T.
  • Journal Title: Mushroom Science and Biotechnology Volume: 22 Pages: 79-85
  • NAID: 110009863114
  • Peer Reviewed
• [Presentation] Immunomodulating effects of the edible mushroom, Pleurotus citrinopileatus, on innate immunocompetent cells such as dendritic cell and macrophage (2017)
  • Author(s): K. Minato, M. Mizuno, L. Laan, A. Ohara, I. van Die
  • Organizer: The 9th International Medicinal Mushrooms Conference
  • Place of Presentation: イタリア
  • Year and Date: 2017-09-24
  • Int'l Joint Research
• [Presentation] Immunomodulating and tumoricidal effects of b-1,3/1,6-glucan from Pleurotus citrinopileatus (2017)
  • Author(s): K. MINATO, L. LAAN, I. van DIE
  • Organizer: Immuno-Oncology-Summit-Europe
  • Place of Presentation: イギリス
  • Year and Date: 2017-03-20
  • Int'l Joint Research
• [Presentation] タモギタケ中βグルカンのマクロファージ分化に対する影響 (2017)
  • Author(s): 湊健一郎，小原章裕
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都
  • Year and Date: 2017-03-17
• [Presentation] マクロファージ分化におけるキノコ由来多糖の影響 (2016)
  • Author(s): 湊健一郎
  • Organizer: 日本応用糖質科学会第42回近畿支部会
  • Place of Presentation: 神戸
  • Year and Date: 2016-11-26
  • Invited
• [Presentation] Immunomodulating and tumoricidal effects of b-1,3/1,6-glucan from Pleurotus citrinopileatus (2016)
  • Author(s): K. MINATO, M. MIZUNO, L. LAAN, I. van DIE
  • Organizer: Novel Cancer Therapeutics Summit
  • Place of Presentation: アメリカ
  • Year and Date: 2016-11-07
  • Int'l Joint Research
• [Presentation] Immunomodulating effects of edible mushrooms on innate immunocompetent cells such as macrophage and dendritic cell (2016)
  • Author(s): K. Minato, L. C. Laan, A. Ohara, I. van Die
  • Organizer: The Food Factor I
  • Place of Presentation: スペイン
  • Year and Date: 2016-11-02
  • Int'l Joint Research
• [Presentation] 自然免疫系細胞に対する機能性多糖およびその他食品成分の免疫調節作用 (2016)
  • Author(s): 湊健一郎
  • Organizer: 第63回日本食品科学工学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2016-08-25
  • Invited
• [Presentation] 自然免疫系細胞に対する機能性多糖およびその他食品成分の免疫調節作用 (2016)
  • Author(s): K. Minato, L. Laan, A. Ohara and I. van Die
  • Organizer: 日本食品科学工学会第63回大会 シンポジウム
  • Place of Presentation: 名古屋市
  • Year and Date: 2016-08-25
• [Presentation] マクロファージ分化におけるビタミンDの影響 (2015)
  • Author(s): 安藤 恵、湊 健一郎、小原 章裕
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山市
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] An Inhibition Effect of Cinnamaldehyde on Inflammation of Microglia (2014)
  • Author(s): Ken-ichiro Minato, Sayaka Matsumoto, Akihiro Ohara
  • Organizer: SFRBM 2014
  • Place of Presentation: Seattle, WA, USA
  • Year and Date: 2014-11-19 – 2014-11-23
• [Presentation] マクロファージにおけるビタミン D 3 の分化への影響 (2014)
  • Author(s): 安藤 恵, 湊 健一郎, 小原章裕
  • Organizer: 日本食品科学工学会 第61回大会
  • Place of Presentation: 福岡市
  • Year and Date: 2014-08-28 – 2014-08-30
• [Presentation] 食用きのこの骨代謝に対する影響 (2014)
  • Author(s): 渡辺 広大、湊 健一郎、小原 章裕
  • Organizer: 第68回日本栄養・食糧学会大会
  • Place of Presentation: 札幌市
  • Year and Date: 2014-05-30 – 2014-06-01