Studies on disposition of vitamin K and development of novel method for nutritional assessment of vitamin K using urine metabolite
| Project/Area Number |
26560072
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
Kamao Maya 神戸薬科大学, 薬学部, 助手 (40299087)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | ビタミンK / phylloquinone / menaquinone-4 / menadione / 抱合体 / 尿中排泄 / ｐhylloquinone |
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| Outline of Final Research Achievements |
Determination method for vitamin K3 (menadione, MD) by LC-MS/MS was developed. MD is thought to be an intermediate in the conversion from vitamin K1 (phylloquinone, PK) to vitamin K2 (menaquinone-4, MK-4). Using this method, we demonstrated that MD was generated from PK or MK-4 in liver, kidney, intestine and bone derived cells. Thus, side-chain cleaving toward vitamin K is assumed to be universal reaction in various tissues. Our results raise the possibility that MD might exist as a glucuronide in human urine mainly and a glutathione or N-acetylcysteine conjugate in mouse plasma.
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Report
(3 results)
Research Products
(3 results)
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[Journal Article] Functional characterization of the vitamin K2 biosynthetic enzyme UBIAD12015
Author(s)
Hirota Y, Nakagawa K, Sawada N, Okuda N, Suhara Y, Uchino Y, Kimoto T, Funahashi N, Kamao M, Tsugawa N, Okano T.
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Journal Title
PLOS ONE.
Volume: 10
Issue: 4
Pages: e0125737-e0125737
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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