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Studies on disposition of vitamin K and development of novel method for nutritional assessment of vitamin K using urine metabolite

Research Project

Project/Area Number 26560072
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Eating habits
Research InstitutionKobe Pharmaceutical University

Principal Investigator

Kamao Maya  神戸薬科大学, 薬学部, 助手 (40299087)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsビタミンK / phylloquinone / menaquinone-4 / menadione / 抱合体 / 尿中排泄 / phylloquinone
Outline of Final Research Achievements

Determination method for vitamin K3 (menadione, MD) by LC-MS/MS was developed. MD is thought to be an intermediate in the conversion from vitamin K1 (phylloquinone, PK) to vitamin K2 (menaquinone-4, MK-4). Using this method, we demonstrated that MD was generated from PK or MK-4 in liver, kidney, intestine and bone derived cells. Thus, side-chain cleaving toward vitamin K is assumed to be universal reaction in various tissues. Our results raise the possibility that MD might exist as a glucuronide in human urine mainly and a glutathione or N-acetylcysteine conjugate in mouse plasma.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (1 results) Book (1 results)

  • [Journal Article] Functional characterization of the vitamin K2 biosynthetic enzyme UBIAD12015

    • Author(s)
      Hirota Y, Nakagawa K, Sawada N, Okuda N, Suhara Y, Uchino Y, Kimoto T, Funahashi N, Kamao M, Tsugawa N, Okano T.
    • Journal Title

      PLOS ONE.

      Volume: 10 Issue: 4 Pages: e0125737-e0125737

    • DOI

      10.1371/journal.pone.0125737

    • NAID

      130007413127

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 培養細胞におけるビタミンKのMenaquinone-4への変換反応と変換中間体Menadioneの生成2016

    • Author(s)
      ○鎌尾 まや,神谷 有紀,長尾 真里,佐方 俊介,山村 翔太,須原 義智,岡野 登志夫,中川 公恵
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Book] ビタミンKと疾患2014

    • Author(s)
      鎌尾まや(岡野登志夫編)
    • Publisher
      医薬ジャーナル社
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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