| Project/Area Number |
26560072
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
Kamao Maya 神戸薬科大学, 薬学部, 助手 (40299087)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | ビタミンK / phylloquinone / menaquinone-4 / menadione / 抱合体 / 尿中排泄 / ｐhylloquinone |
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| Outline of Final Research Achievements |
Determination method for vitamin K3 (menadione, MD) by LC-MS/MS was developed. MD is thought to be an intermediate in the conversion from vitamin K1 (phylloquinone, PK) to vitamin K2 (menaquinone-4, MK-4). Using this method, we demonstrated that MD was generated from PK or MK-4 in liver, kidney, intestine and bone derived cells. Thus, side-chain cleaving toward vitamin K is assumed to be universal reaction in various tissues. Our results raise the possibility that MD might exist as a glucuronide in human urine mainly and a glutathione or N-acetylcysteine conjugate in mouse plasma.
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