| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Outline of Final Research Achievements |
We tested whether oral 5'-adenosine monophosphate (AMP), an umami substance in taste related to a nucleotide, administration alleviates symptoms of chronic colitis induced by dextran sodium sulfate (DSS) in mice. AMP administration significantly reduced the severity of colitis, such as watery and bloody diarrhea in mice and attenuated mucosal tissue damages in the colon. Furthermore, mice given AMP had higher expression of phosphorylated AMP-activated protein kinase and lower levels of pro-inflammatory cytokines (TNF-alpa, INF-gamma, and IL-17A) in the colon tissues.
Collectively, these findings suggested that oral AMP administration improves the severity of DSS-induced chronic colitis in mice via the activation of AMPK. Interestingly, 5’-inosine monophosphate also had similar anti-inflammatory effects, but not 5’-guanosine monophosphate. However, the mechanisms of anti-inflammatory effects with the umami nucleotides remain to be elucidated.
|
