In-situ observation system for examination of nonfreezing preservation of living cells under high hydrostatic pressure.
| Project/Area Number |
26560203
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Terano Motoki 東京工業大学, 大学院理工学研究科, 助教 (90708554)
Yamamoto Takatoki 東京工業大学, 大学院理工学研究科, 准教授 (20322688)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 生物生体工学 / 生体物性 / 細胞保存 / 冷却 / 高圧 / 過冷却 / 卵 / 不凍結冷却 / 高静水圧 / 凝固点降下 / その場観察 / 氷点下 / 損傷 |
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| Outline of Final Research Achievements |
For experimental study on nonfreezing preservation of living cells under high hydrostatic pressure, an experimental device for in-situ observation of a chamber that can control hydrostatic pressure and temperature (lower than 0℃), was developed. It was confirmed that the device has enough performance of load pressure, temperature-control, and in-situ observation.
Living eggs of killifish were employed for freezing experiment. Freezing processes of eggs were observed under various hydrostatic pressure using the developed device. Also, damage of eggs were evaluated by hatch status of the eggs. It was found from the experiments that a hatch limit depended on the pressure and the temperature, and that the pressure and temperature history influenced the hatch limit.
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Report
(3 results)
Research Products
(5 results)
