Control of cell differentiation by ultrasound through epigenetic mechanisms
Project/Area Number |
26560205
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Biomedical engineering/Biomaterial science and engineering
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Research Institution | University of Toyama |
Principal Investigator |
TABUCHI YOSHIAKI 富山大学, 研究推進機構 研究推進総合支援センター, 教授 (20322109)
|
Co-Investigator(Kenkyū-buntansha) |
近藤 隆 富山大学, 大学院医学薬学研究部(医学), 教授 (40143937)
池亀 美華 岡山大学, 医歯薬学総合研究科, 准教授 (70282986)
鈴木 信雄 金沢大学, 環日本海域環境研究センター, 教授 (60242476)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
|
Keywords | 超音波 |
Outline of Final Research Achievements |
Using fish scales in which osteoclasts and osteoblasts coexist, we identified that mRNA expression levels of receptor activator of nuclear factor kappa-B ligand (RANKL), matrix metalloproteinase-9 (MMP-9) and cathepsin K, important molecules of osteoclast activation, were significantly increased with a single low-intensity pulsed ultrasound (LIPUS; 30 mW/cm2, 20 min) treatment. Moreover, 2 weeks after daily LIPUS exposure, either the osteoblastic marker enzyme activity or the regeneration rate of the scales was significantly increased in LIPUS-treated groups. In mouse preosteoblast MC3T3-E1 cells, the protein expression level of trimethyl histone H3 (Lys9) was significantly elevated in the cells treated with a single LIPUS exposure. These findings will provide a molecular basis for further understanding of the mechanisms of LIPUS in bone cells.
|
Report
(4 results)
Research Products
(9 results)
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[Journal Article] <b>Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of </b><b>bone </b>2017
Author(s)
Hanmoto T, Tabuchi Y, Ikegame M, Kondo T, Kitamura KI, Endo M, Kobayashi I, Mishima H, Sekiguchi T, Urata M, Seki A, Yano S, Hattori A, Suzuki N.
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Journal Title
Biomedical Research
Volume: 38
Issue: 1
Pages: 71-77
DOI
NAID
ISSN
0388-6107, 1880-313X
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] <b>Tensile stress stimulates the expression of osteogenic cytokines/growth factors and matricellular proteins in the mouse cranial suture at the site of osteoblast differ</b><b>entiation </b>2016
Author(s)
Mika Ikegame, Yoshiaki Tabuchi, Yukihiro Furusawa, Mariko Kawai, Atsuhiko Hattori, Yoshiaki Tabuchi, Yukihiro Furusawa, Mariko Kawai, Atsuhiko Hattori, Takashi Kondo, Tos Yukihiro Furusawa, Mariko Kawai, Atsuhiko Hattori, Takashi Kondo, Toshio Yamamoto
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Journal Title
Biomedical Research
Volume: 37
Issue: 2
Pages: 117-126
DOI
NAID
ISSN
0388-6107, 1880-313X
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Low-intensity pulsed ultrasound induces apoptosis in osteoclasts: Fish scales are a suitable model for the analysis of bone metabolism by ultrasound.2016
Author(s)
Suzuki N, Hanmoto T, Yano S, Furusawa Y, Ikegame M, Tabuchi Y, Kondo T, Kitamura K, Endo M, Yamamoto T, Sekiguchi T, Urata M, Mikuni-Takagaki Y, Hattori A.
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Journal Title
Comp. Biochem. Physiol. Part A
Volume: 195
Pages: 26-31
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of bone.2016
Author(s)
Hanmoto T, Tabuchi Y, Ikegame M, Kondo T, Kitamura K, Endo M, Mishima H, Sekiguchi T, Urata M, Seki A, Yano S, Hattori A, Suzuki N.
Organizer
The Joint Meeting of the 22nd International Congress of Zoology & the 87th Meeting of the Zoological Society of Japan
Place of Presentation
Ginowan, Japan
Year and Date
2016-11-14
Related Report
Int'l Joint Research
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