Heart Tissue Engineering: Integration of microfabrication technology and THz wave

• Project/Area Number: 26560209
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kyoto University
• Principal Investigator: Kamei Ken-ichiro 京都大学, 物質－細胞統合システム拠点, 特定拠点准教授 (00588262)
• Co-Investigator(Renkei-kenkyūsha): 廣理 英基 京都大学, 物質-細胞統合システム拠点, 特定拠点准教授 (00512469)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 幹細胞 / マイクロ流体デバイス / THz / 組織工学 / 心筋細胞 / 細胞工学 / iPS細胞 / 顕微鏡 / テラヘルツ波 / ヒトES/iPS細胞 / 心筋 / 分化誘導 / 成熟化

Outline of Final Research Achievements

We aimed to develop a new platform to obtain functional and mature cardiomyocytes derived from human embryonic stem (ES) and induced pluripotent stem (iPS) cells by utilizing a microfluidic device in combination with THz wave. Specifically, A. to develop a microfluidic device allowing three-dimensional cell culture for cardiomyocytes, B. to combine the experimental setups of both THz microscope and microfluidic devices, C. to make mature cardiomyocytes by irradiating THz wave. Our devices would be very beneficial for regenerative medicine as well as drug screening for heart diseases.

Research Products

• [Journal Article] Microfluidic-nanofiber hybrid array for screening of cellular microenvironments (2017)
  • Author(s): K. Kamei, Y. Mashimo, M. Yoshioka, Y. Tokunaga, C. Fockenberg, S. Terada, M. Nakajima, T. Shibata-Seki, L. Liu, T. Akaike, E. Kobatake, E. S. How, M. Uesugi and Y. Chen
  • Journal Title: Small Volume: 印刷中 Issue: 18 Pages: 1603104-1603104
  • DOI: 10.1002/smll.201603104
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 3次元微細加工を応用したBody on a Chip の開発 (2016)
  • Author(s): 加藤義基, 平井義和, 亀井謙一郎, 土屋智由, 田畑修
  • Journal Title: 電気学会論文誌（センサ・マイクロマシン部門誌） Volume: 136
  • NAID: 40020858740
  • Peer Reviewed
• [Journal Article] Directing and boosting of cell migration by the entropic force gradient in polymer solution (2015)
  • Author(s): T. Fukuyama, A. Fuke, M. Mochizuki, K. Kamei and Y. T. Maeda
  • Journal Title: Langmuir Volume: 31 Issue: 46 Pages: 12567-12572
  • DOI: 10.1021/acs.langmuir.5b02559
  • Peer Reviewed
• [Journal Article] Oscillation and collective conveyance of water-in-oil droplets by microfluidic bolus flow (2015)
  • Author(s): T. Ohnuma, M. Ichikawa, K. Kamei, Y. T. Maeda
  • Journal Title: Applied Physics Letters Volume: 107 Issue: 7 Pages: 074102-074102
  • DOI: 10.1063/1.4928753
  • Peer Reviewed
• [Presentation] Microfluidic device to interconnect multiple organs via fluidic circulation: Towards Body-on-a-Chip (2015)
  • Author(s): Y. Kato, Y. Hirai, K. Kamei, T. Tsuchiya, O. Tabata
  • Organizer: The 18th International Conference on Solid-State Sensors Actuators and Microsystems (Transducers’15)
  • Place of Presentation: Anchorage, AK, USA
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Book] Methods Mol. Biol. (2015)
  • Author(s): Y. Mashimo, K. Kamei
  • Total Pages: 14
  • Publisher: Springer
• [Book] Methods in Molecular Biology (2015)
  • Author(s): Yasumasa Mashimo, Ken-ichiro Kamei
  • Publisher: Springer