Evaluation of minor histocompatibility antigens by kidney transplant model derived from somatic cell cloned animals

• Project/Area Number: 26560212
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: The University of Tokushima (2015-2016); Yamaguchi University (2014)
• Principal Investigator: Otoi Takeshige 徳島大学, 大学院生物資源産業学研究部, 教授 (30311814)
• Co-Investigator(Kenkyū-buntansha): 久保 正仁 山口大学, 共同獣医学部, 助教 (40593439) ; 原口 友也 山口大学, 共同獣医学部, 助教 (90634759)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: クローン / 腎移植 / ミトコンドリア / 拒絶反応

Outline of Final Research Achievements

We tried to examine the effect of different mitochondrial DNA (mtDNA) by the transplantation of organ to donors that provided somatic cells using cloned animals. However, we could not obtain cloned cats for transplantation of organs to somatic cell donor cats.Thus, the effect of trichostatin A (TSA) on the development of interspecies cloned embryos (cat somatic cells / porcine or bovine enucleated oocytes) was investigated. TSA treatment had beneficial effects on the development of Cat-Cow cloned embryos but not of Cat-Pig cloned embryos. These results indicate that the difference in mitochondrial DNA may affect the development of cloned embryos.

Research Products

• [Journal Article] Epigenetic modulation on Cat-Cow interspecies somatic cell nuclear transfer embryos by treatment with Trichostatin A. (2017)
  • Author(s): Wittayarat, M., Sato, Y., Do, T.K.L., Chatdarong, K., Tharasanit, T., Techakumphu, M., Taniguchi, M. and Otoi, T.
  • Journal Title: Anim. Sci. J. Volume: 88 Issue: 4 Pages: 593-601
  • DOI: 10.1111/asj.12676
  • NAID: 120007099360
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of duration of electric pulse on in vitro development of cloned cat embryos with human artificial chromosome vector. (2016)
  • Author(s): Do L, Wittayarat M, Terazono T, Sato Y, Taniguchi M, Tanihara F, Takemoto T, Kazuki Y, Kazuki K, Oshimura M, Otoi T.
  • Journal Title: Reprod Domest Anim Volume: 51 Issue: 6 Pages: 1039-1043
  • DOI: 10.1111/rda.12766
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Cell cycle analysis and interspecies nuclear transfer of cat cells treated with chemical inhibitors. (2014)
  • Author(s): Wittayarat M, Fujiwara A, Chatdarong K, Techakumphu M, Sato Y, Tanihara F, Morita Y, Taniguchi M,and Otoi T.
  • Journal Title: Acta Vet Hung. Volume: 62 Pages: 233-242
  • Peer Reviewed / Acknowledgement Compliant