| Project/Area Number |
26560221
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Keio University |
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Principal Investigator |
Tsukada Kosuke 慶應義塾大学, 理工学部, 准教授 (00351883)
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| Co-Investigator(Renkei-kenkyūsha) |
SUEMATSU Makoto 慶應義塾大学, 医学部, 教授 (00206385)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | レーザ / ワクチン / アジュバント / 活性酸素 / 免疫 |
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| Outline of Final Research Achievements |
The vaccine adjuvant is an essential component for most of modern vaccines to be effective. However, there is a paucity of both safe and effective vaccine adjuvant. Recently, near-infrared (NIR) laser light has been shown to significantly enhancing vaccine responses and can be used as vaccine adjuvant by others and us. However, critical pieces of information to advance this technology to the point of advanced pre-clinical development are still largely missing.
We developed a laser system to irradiate cultured cells with flat beam profile. We cultured human epithelial keratinocytes and measured CCL20 expressions with qRT-PCR after laser irradiations. Next, we focused on mitochondrial functions and established measurement methods to quantify reactive oxygen species with ESR and their oxygen consumption with oxygen sensor, respectively. These quantitative analysis will reveal the detailed mechanisms on immune activation with near infrared laser.
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