| Project/Area Number |
26560222
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Keio University |
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Principal Investigator |
Miyata Shogo 慶應義塾大学, 理工学部, 准教授 (70376515)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 毛髪再生 / 生体組織工学 / 三次元培養 / 共培養 / 再生医療 / 再生医療工学 / コラーゲンゲル / 毛包 / 生体外再生 / 毛包再生 / マイクロインジェクション / 多層ビーズ |
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| Outline of Final Research Achievements |
The purpose of this study is to regenerate hair tissue in vitro by controlling three-dimensional cellular organization. To simulate hair follicle structure, collagen gel including two kinds of cells was multi-layered to construct three-dimensional structure.
In this study, mouse embryonic stem cells (mESCs) suspended in collagen gel were micro-spotted and over-layered by mouse fatal epidermal cells suspended in collagen. After the gel beads spotting, cell-seeded collagen gels were cultured in vitro. As the results, hair follicle was regenerated in vitro using our novel culture method. Moreover, arrector pili muscle-like tissue was also regenerated.
In conclusion, three-dimensional culture method simulating hair follicle structure was newly developed. Using our novel method, micro-spotted gel-cultured specimen indicated capability to regenerate follicle by fully-in vitro culture process.
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