| Project/Area Number |
26560225
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
Fukuda Norio 東京慈恵会医科大学, 医学部, 准教授 (30301534)
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| Co-Investigator(Kenkyū-buntansha) |
照井 貴子 東京慈恵会医科大学, 医学部, 助手 (10366247)
小比類巻 生 東京慈恵会医科大学, 医学部, 助教 (40548905)
大山 廣太郎 東京慈恵会医科大学, 医学部, 特別研究員 (70632131)
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| Co-Investigator(Renkei-kenkyūsha) |
ISHIWATA SHIN'ICHI 早稲田大学, 理工学術院, 名誉教授 (10130866)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | ナノバイオ / 先端機器デバイス / 循環器・高血圧 / 分子イメージング / 先端機能デバイス |
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| Outline of Final Research Achievements |
1）We developed an experimental system for simultaneous nano-imaging of intracellular Ca dynamics and single sarcomere length in rat neonatal cardiomyocytes. We achieved this by expressing a fluorescence resonance energy transfer-based Ca sensor yellow Cameleon-Nano fused to α-actinin in order to localize to the Z disks. 2）We developed a high-speed (100 fps), high-resolution (20 nm) nano-imaging system for myocardial sarcomeres in living mice. During imaging, we measured macroscopic parameters such as electrocardiogram and left ventricular pressure. 3) We developed a digitally-controlled image reconstruction system for in vivo nano-imaging. Namely, the position of the objective lens was moved downward in the Z-direction during imaging. After experimentation, the LVP record was divided into 17 phases (i.e., 10.2 ms per phase); best-focused images were selected at each phase, and then, combined to obtain an image sequence.
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