Polymeric nanosheets for cell and drug delivery and in vivo local monitoring

• Project/Area Number: 26560233
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tohoku University
• Principal Investigator: Kaji Hirokazu 東北大学, 工学(系)研究科(研究院), 准教授 (70431525)
• Co-Investigator(Renkei-kenkyūsha): ISHIMURA KOSEI 独立行政法人宇宙航空研究開発機構, 宇宙科学研究所, 准教授 (10333626) ; FUJIE TOSHINORI 早稲田大学, 理工学術院, 助教 (70538735) ; NAGAMINE KUNIAKI 東北大学, 大学院工学研究科, 助教 (00551540)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 細胞・組織工学材料

Outline of Final Research Achievements

Multifunctional cell culture scaffolds consisting of flexible, ultrathin polymeric films (nanosheets) have been developed toward cell transplantation therapy for intractable retinal diseases such as age-related macular degeneration. Retinal pigment epithelial (RPE) cells cultured on biodegradable nanosheets formed a monolayer with hexagonal tight junctions found in native tissues. It is found that those cell/nanosheet composites can be aspirated and injected by a syringe needle without significant loss of cell morphology and viability. Also, the ability to release drugs and to sense oxygen and pH could be given to nanosheets by loading functionalized nanoparticles into nanosheets. The developed nanosheets would be expected as a biomedical device to support stem cell therapy.

Research Products

• [Journal Article] Protective effects of transscleral sustained unoprostone delivery against retinal degeneration in S334ter rhodopsin mutant rats (2015)
  • Author(s): Nobuhiro Nagai, Satoru Iwata, Kaori Sampei, Hideyuki Onami, Hirokazu Kaji, Matsuhiko Nishizawa, Toru Nakazawa, Yukihiko Mashima, Toshiaki Abe
  • Journal Title: Journal of Biomedical Materials Research Part B: Applied Biomaterials Volume: in press Issue: 8 Pages: 1730-1737
  • DOI: 10.1002/jbm.b.33522
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Application of clotrimazole via a novel controlled release device provides potent retinal protection (2015)
  • Author(s): Zhaleh Kashkouli Nezhad, Nobuhiro Nagai, Kotaro Yamamoto, Hirokazu Kaji, Matsuhiko Nishizawa, Hideyuki Saya, Toru Nakazawa, Toshiaki Abe
  • Journal Title: J Mater Sci Mater Med Volume: 26 Issue: 9 Pages: 5561-5561
  • DOI: 10.1007/s10856-015-5561-9
  • Peer Reviewed / Open Access
• [Journal Article] A polymeric device for controlled transscleral multi-drug delivery to the posterior segment of the eye (2014)
  • Author(s): Nobuhiro Nagai, Hirokazu Kaji, Hideyuki Onami, Yumi Ishikawa, Matsuhiko Nishizawa, Noriko Osumi, Toru Nakazawa, Toshiaki Abe
  • Journal Title: Acta Biomaterialia Volume: 10(2) Issue: 2 Pages: 680-6877
  • DOI: 10.1016/j.actbio.2013.11.004
  • NAID: 40020425298
  • Peer Reviewed
• [Journal Article] Micropatterned polymeric nanosheets for local delivery of an engineered epithelial monolayer (2014)
  • Author(s): Toshinori Fujie, Yoshihiro Mori, Shuntaro Ito, Matsuhiko Nishizawa, Hojae Bae, Nobuhiro Nagai, Hideyuki Onami, Toshiaki Abe, Ali Khademhosseini, Hirokazu Kaji
  • Journal Title: Adv. Mater Volume: 26 Issue: 29 Pages: 1699-1705
  • DOI: 10.1002/adma.201301300
  • Peer Reviewed
• [Journal Article] A Platform for Controlled Dual-Drug Delivery to the Retina : Protective Effects against Light-Induced Retinal Damage in Rats (2014)
  • Author(s): N Nagai, H Kaji, H Onami, Y Katsukura, Y Ishikawa, ZK Nezhad, K Sampei, S Iwata, S Ito, M Nishizawa, T Nakazawa, N Osumi, Y Mashima, T Abe
  • Journal Title: Advanced Healthcare Materials Volume: in press Issue: 10 Pages: 680-687
  • DOI: 10.1002/adhm.201400114
  • Peer Reviewed / Open Access
• [Presentation] 高分子薄膜を用いたインジェクション可能な薬剤徐放デバイスの開発 (2016)
  • Author(s): 近藤太郎, Zhaleh Kashkouli Nezhad, 綱島俊一, 陳俐君, 永井展裕, 西澤松彦, 阿部俊明, 梶 弘和
  • Organizer: 第28回バイオエンジニアリング講演会
  • Place of Presentation: 東京工業大学、東京
  • Year and Date: 2016-01-09
• [Presentation] 自己展開型薬剤徐放高分子デバイスの開発 (2015)
  • Author(s): 近藤太郎, Zhaleh Kashkouli Nezhad, 永井展裕, 西澤松彦, 阿部俊明, 梶 弘和
  • Organizer: 化学とマイクロ・ナノシステム学会
  • Place of Presentation: 北九州国際会議場、福岡
  • Year and Date: 2015-11-26
• [Presentation] Polymeric thin films for cell and drug delivery (2015)
  • Author(s): Hirokazu Kaji
  • Organizer: IEEE-NANOMED 2015
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2015-11-15
  • Int'l Joint Research / Invited
• [Presentation] 高分子薄膜を用いた自己展開型薬剤徐放デバイスの開発 (2015)
  • Author(s): 近藤太郎, Zhaleh Kashkouli Nezhad, 綱嶋俊一, 陳 俐君, 永井展裕, 西澤松彦, 阿部俊明, 梶 弘和
  • Organizer: 第37回日本バイオマテリアル学会大会
  • Place of Presentation: 京都
  • Year and Date: 2015-11-09
• [Presentation] ミクロ細孔を有するタンパク質徐放デバイスに関する検討 (2015)
  • Author(s): 山田慎二, 永井展裕, 勝山 綾, 泉田泰子, 小柳恵理, 梶 弘和, 後藤昌史, 阿部俊明
  • Organizer: 第37回日本バイオマテリアル学会大会
  • Place of Presentation: 京都テルサ、京都
  • Year and Date: 2015-11-09
• [Presentation] Injectable nanosheets as promising drug delivery systems for retinal disorders (2015)
  • Author(s): Zhaleh Kashkouli Nezhad, 近藤太郎, 梶 弘和, 永井展裕, 西澤松彦, 阿部俊明
  • Organizer: 第37回日本バイオマテリアル学会大会
  • Place of Presentation: 京都テルサ、京都
  • Year and Date: 2015-11-09
• [Presentation] 眼局所持続性抗がん剤徐放デバイスの開発 (2015)
  • Author(s): 永井展裕, 勝山 綾, 泉田泰子, 小柳恵理, 山田慎二, 梶 弘和, 阿部俊明
  • Organizer: 第37回日本バイオマテリアル学会大会
  • Place of Presentation: 京都テルサ、京都
  • Year and Date: 2015-11-09
• [Presentation] Injectable ultrathin polymeric films for subretinal cell delivery (2015)
  • Author(s): Hirokazu Kaji, Taro Kondo, Nobuhiro Nagai, Toshiaki Abe
  • Organizer: 2015 BMES Annual Meeting
  • Place of Presentation: Tampa, Florida, USA
  • Year and Date: 2015-10-07
  • Int'l Joint Research
• [Presentation] Injectable polymeric nanosheets for subretinal cell delivery (2015)
  • Author(s): Hirokazu Kaji, Taro Kondo, Yoshihiro Mori, Nobuhiro Nagai, Toshiaki Abe
  • Organizer: 4th TERMIS World Congress
  • Place of Presentation: Boston, MA, USA
  • Year and Date: 2015-09-08
  • Int'l Joint Research
• [Presentation] 高分子薄膜を用いた薬剤徐放デバイスの開発 (2015)
  • Author(s): 近藤太郎, Zhaleh Kashkouli Nezhad, 永井展裕, 西澤松彦, 阿部俊明, 梶 弘和
  • Organizer: 第31回日本DDS学会学術集会
  • Place of Presentation: 京王プラザホテル、東京
  • Year and Date: 2015-07-02
• [Presentation] Cell manipulation based on micro/nanotechnologies and biomedical application (2015)
  • Author(s): Hirokazu Kaji
  • Organizer: 7th International Symposium on Microchemistry and Microsystems (ISMM)
  • Place of Presentation: Kyoto
  • Year and Date: 2015-06-08
  • Int'l Joint Research / Invited
• [Presentation] マイクロ・ナノ技術を用いた細胞操作と医工領域への展開 (2015)
  • Author(s): 梶 弘和
  • Organizer: 電気化学会第82回大会
  • Place of Presentation: 横浜、横浜国立大学
  • Year and Date: 2015-03-15 – 2015-03-17
  • Invited
• [Presentation] ラット眼球網膜下への細胞担持ナノシートデリバリー (2014)
  • Author(s): 森 好弘, 藤枝俊宣, 永井展裕, 西澤松彦, 阿部俊明, 梶 弘和
  • Organizer: 第36回日本バイオマテリアル学会大会
  • Place of Presentation: 東京、タワーホール船堀
  • Year and Date: 2014-11-17 – 2014-11-18
• [Presentation] ナノシートを用いる眼内細胞送達システムの開発 (2014)
  • Author(s): 森 好弘, 藤枝俊宣, 永井展裕, 西澤松彦, 阿部俊明
  • Organizer: 第30回日本DDS学会学術集会
  • Place of Presentation: 東京、慶應義塾大学
  • Year and Date: 2014-07-30 – 2014-07-31
• [Presentation] Local delivery of an engineered epithelial monolayer by micropatterned polymeric Nanosheets (2014)
  • Author(s): Hirokazu Kaji, Toshinori Fujie, Nobuhiro Nagai, Toshiaki Abe
  • Organizer: ARVO 2014 Annual Meeting
  • Place of Presentation: Orland, Florida, USA
  • Year and Date: 2014-05-04 – 2014-05-08
• [Presentation] Cell delivery system using micropatterned polymeric nanosheets (2014)
  • Author(s): Hirokazu Kaji, Toshinori Fujie, Yoshinori Mori, Nobuhiro Nagai, Ali Khademhosseini, Toshiaki Abe
  • Organizer: 2014 Annual Meeting of the Society For Biomaterials
  • Place of Presentation: Denver, CO, USA
  • Year and Date: 2014-04-16 – 2014-04-19
• [Patent(Industrial Property Rights)] 薬剤徐放デバイス (2015)
  • Inventor(s): 梶弘和,近藤太郎,ジャレカシュクリネジャド,永井展裕,阿部俊明
  • Industrial Property Rights Holder: 梶弘和,近藤太郎,ジャレカシュクリネジャド,永井展裕,阿部俊明
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-110747
  • Filing Date: 2015-05-29
• [Patent(Industrial Property Rights)] 形状制御されたナノシート及びその製造方法 (2015)
  • Inventor(s): 岩瀬英治,新保創太,武岡真司,藤枝俊宣,梶弘和,阿部俊明
  • Industrial Property Rights Holder: 岩瀬英治,新保創太,武岡真司,藤枝俊宣,梶弘和,阿部俊明
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-043990
  • Filing Date: 2015-03-05
• [Patent(Industrial Property Rights)] 細胞担持パターン化ナノ薄膜 (2014)
  • Inventor(s): 梶弘和,藤枝俊宣,森好弘,西澤松彦,阿部俊明,永井展裕
  • Industrial Property Rights Holder: 梶弘和,藤枝俊宣,森好弘,西澤松彦,阿部俊明,永井展裕
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-06-27
  • Overseas