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Development of serum-free medium and the establishment of serum-independent cell lines

Research Project

Project/Area Number 26560242
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionKagawa University

Principal Investigator

Nakamura Takanori  香川大学, 医学部, 教授 (70183887)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords血清 / 無血清 / FBS / リポタンパク質 / アルブミン / 有機酸 / T細胞 / LDL / Jurkat
Outline of Final Research Achievements

In this study, it was an objective to identify and profile the protein factors, lipids and organic acids included in the animal serum, participating in the cell proliferation and/or growth inhibition by using T-cell lines. In addition, we planned the improvement of the cell culture method to rely on in 100% import serum (FBS). As a result, it was thought that lipoprotein such as the LDL was effective for the cell proliferation. In addition, the promotional effect was accepted by organic acids including citric acid and pyruvic acid and was able to confirm that the recombinant albumin was also an important promotional factor in the cell culture. Furthermore, after analyzing it, we understood that cholesterol was the most important lipid and was able to prepare a trial product of the low protein serum-free medium by adding in, transferrin, selenious acid and recombinant albumin.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (8 results)

  • [Journal Article] Crystal structure of a Xenopus laevis skin proto-type galectin, close to but distinct from galectin-12015

    • Author(s)
      Nonaka Y, Ogawa T, Yoshida H, Shoji H, Nishi N, Kamitori S, Nakamura T
    • Journal Title

      Glycobiology

      Volume: 印刷中 Issue: 7 Pages: 792-803

    • DOI

      10.1093/glycob/cwv020

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] ツメガエル皮膚ガレクチンの立体構造とアロステリックな挙動についての解析2015

    • Author(s)
      野中康宏, 小川崇, 吉田裕美, 東海林博樹, 西望, 神鳥成弘, 中村隆範
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会(合同大会)
    • Place of Presentation
      神戸市
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Functional and expression analysis of Galectin-4 in Xenopus digestive tract and human colon carcinoma2015

    • Author(s)
      小川 崇,東海林 博樹,野中 康宏, 舘野 浩章, 平林 淳, 西 望,中村隆範
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会(合同大会)
    • Place of Presentation
      神戸市
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] ツメガエル皮膚ガレクチンの構造・機能解析とgalectin-1との比較2015

    • Author(s)
      野中康宏, 小川崇, 吉田裕美, 東海林博樹, 西望, 神鳥成弘, 中村隆範
    • Organizer
      第15回日本蛋白質科学会年会
    • Place of Presentation
      徳島市
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] ツメガエル由来の二種のプロト型ガレクチンついてのX線結晶構造解析2015

    • Author(s)
      野中康宏, 小川崇, 吉田裕美, 東海林博樹, 西望, 神鳥成弘, 中村隆範
    • Organizer
      第14回四国免疫フォーラム
    • Place of Presentation
      愛媛県東温市
    • Year and Date
      2015-06-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] アフリカツメガエル皮膚由来ガレクチンの立体構造と機能の解析2014

    • Author(s)
      野中康宏, 小川崇, 吉田裕美, 東海林博樹, 西望, 神鳥成弘, 中村隆範
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] K562細胞の増殖における必要因子について2014

    • Author(s)
      山下賀容子, 中村隆範
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] ツメガエル消化管およびヒト大腸がん細胞におけるガレクチン4の発現及び機能解析2014

    • Author(s)
      小川 崇,東海林 博樹,野中 康宏, 舘野 浩章, 平林 淳, 西 望,中村隆範
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] アフリカツメガエルの皮膚および遍在型ガレクチンの立体構造解析2014

    • Author(s)
      野中康宏, 小川崇, 吉田裕美, 東海林博樹, 西望, 神鳥成弘, 中村隆範
    • Organizer
      第14回日本蛋白質科学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-06-25 – 2014-06-27
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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