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Molecular evolution and functional analysis of influenza virus-binding peptides

Research Project

Project/Area Number 26560245
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionKeio University

Principal Investigator

Sato Toshinori  慶應義塾大学, 理工学部, 教授 (00162454)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsペプチド / インフルエンザウイルス / ヘマグルチニン / ファージディスプレイ法 / 等温滴定型カロリメーター / 感染阻害剤 / ダイヤモンド電極 / ウイルスセンシング / 感染阻害活性 / 熱力学解析 / エンタルピー
Outline of Final Research Achievements

For taking measures against pandemic by emerging of new strains of influenza virus (IFV), we carried out the development of virus detection method and infection inhibitor using IFV-binding peptides. IFV hemagglutinin (HA)-binding peptides were selected from a phage-displayed peptide library. Thermodynamic parameters for the interaction between peptides and HA were determined by isothermal titration calorimetry, and it was indicated that pentapeptides suitable for the size of the receptor-binding pocket in HA showed the similar enthalpy change with pentadecapeptides. The pentapeptide ARLPR showed high infection inhibition activity against major IFV subtypes. Furthermore, by the immobilization of the HA-binding peptides on diamond electrode, we could achieve highly sensitive detection of IFV.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (12 results)

All 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Multivalent effect in influenza hemagglutinin-binding activity of sugar-mimic peptide2016

    • Author(s)
      Teruhiko Matsubara, Ai Onishi, Tomomi Saito, Daisuke Yamaguchi, and Toshinori Sato
    • Journal Title

      Jap. J. Polym. Sci. Tech.

      Volume: 75 Pages: 62-68

    • NAID

      130005120382

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heptapeptide ligands against receptor-binding sites of influenza hemagglutinin toward anti-influenza therapy2016

    • Author(s)
      Teruhiko Matsubara, Ai Onishi, Daisuke Yamaguchi, Toshinori Sato
    • Journal Title

      Bioorg. Med. Chem.

      Volume: 24 Issue: 5 Pages: 1106-1114

    • DOI

      10.1016/j.bmc.2016.01.039

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Binding of Hemagglutinin and Influenza Virus to a Peptide-Conjugated Lipid Membrane2016

    • Author(s)
      Teruhiko Matsubara, Rabi Shibata, Toshinori Sato
    • Journal Title

      Front. Microbiol.

      Volume: 7 Pages: 468-468

    • DOI

      10.3389/fmicb.2016.00468

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Thermodynamic analysis for the interaction between sugar-mimic peptide and hemagglutinin2015

    • Author(s)
      Shiori Yoshikawa, Ryunosuke Kuriyama, Haruka Kori, Teruhiko Matsubara, Toshinori Sato
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      ハワイコンベンションセンター(ホノルル)
    • Year and Date
      2015-12-14
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Electrochemical detection of influenza virus using hemagglutinin-binding peptide-modified diamond electrode2015

    • Author(s)
      Michiko Ujie, Miku Akahori, Teruhiko Matsubara, Takashi Yamamoto, Yasuaki Einaga, Toshinori Sato
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      ハワイコンベンションセンター(ホノルル)
    • Year and Date
      2015-12-14
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 糖鎖模倣ペプチド固定化ダイヤモンド電極によるインフルエンザウイルスの電気化学的検出2015

    • Author(s)
      松原 輝彦・氏江 美智子・赤堀 未来・山本 崇史・栄長 泰明・佐藤 智典
    • Organizer
      第64回高分子討論会
    • Place of Presentation
      東北大学(仙台市・青葉区)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] 糖鎖ミミックペプチドとヘマグルチニンとの相互作用の熱力学的解析2015

    • Author(s)
      吉川 栞、栗山 龍之介、郡 遥香、松原 輝彦、佐藤 智典
    • Organizer
      第25回バイオ・高分子シンポジウム
    • Place of Presentation
      東京工業大学(東京都・目黒区)
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] ペプチド脂質の固定化によるインフルエンザヘマグルチニンおよびウイルスの検出2015

    • Author(s)
      松原 輝彦、柴田 ラビ、佐藤 智典
    • Organizer
      第25回バイオ・高分子シンポジウム
    • Place of Presentation
      東京工業大学(東京都・目黒区)
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 糖鎖ミミックペプチドとヘマグルチニンとの相互作用の熱力学的解析2015

    • Author(s)
      吉川 栞・栗山 龍之介・郡 遥香・松原 輝彦・佐藤 智典
    • Organizer
      高分子学会第25回バイオ・高分子シンポジウム
    • Place of Presentation
      東京工業大学(東京都・目黒区)
    • Year and Date
      2015-07-23
    • Related Report
      2014 Research-status Report
  • [Presentation] 糖鎖ミミックペプチドとヘマグルチニンとの相互作用の熱力学的考察2015

    • Author(s)
      吉川 栞、栗山 龍之介、郡 遥香、松原 輝彦、佐藤 智典
    • Organizer
      第64回高分子学会年次大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2015-05-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 糖鎖ミミックペプチドとヘマグルチニンとの相互作用の熱力学的考察2015

    • Author(s)
      吉川 栞・栗山 龍之介・郡 遥香・松原 輝彦・佐藤 智典
    • Organizer
      第64回高分子学会年次大会
    • Place of Presentation
      札幌コンベンションセンター(札幌市・白石区)
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] インフルエンザウイルス感染阻害剤、リポソーム、 インフルエンザ予防・治療剤2016

    • Inventor(s)
      佐藤 智典・中屋 隆明
    • Industrial Property Rights Holder
      佐藤 智典・中屋 隆明
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-010162
    • Filing Date
      2016-01-21
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2017-05-10  

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