Direct molecular delivery into nucleus by spatiotemporally controlled enhanced Mie scattering light
| Project/Area Number |
26560263
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical systems
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | フェムト秒レーザー / ドラッグデリバリー / レーザー医療 / 低侵襲治療システム |
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| Outline of Final Research Achievements |
High-throughput processing of cell nuclear membrane was investigated by using ultrafast enhanced Mie scattering light. Simultaneous laser ablation of cell membrane and nuclear membrane was also studied for achieving direct molecular delivery into nucleus. The fluorescence of the plasmid DNA/liposome complexes was detected in the nucleus as well as cytoplasm after laser illumination for microspheres. The direct delivery of complexes into the nucleus is probably attributed to the enhancement of the nuclear membrane permeability by the enhanced optical field obtained close to the nucleus.
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Report
(4 results)
Research Products
(15 results)
