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Elucidation of locus specific chromatin complex formation and energy expenditure gene expressions using enChIP

Research Project

Project/Area Number 26560395
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied health science
Research InstitutionThe University of Tokyo

Principal Investigator

SAKAI Juro  東京大学, 先端科学技術研究センター, 教授 (80323020)

Co-Investigator(Renkei-kenkyūsha) ABURATANI Hiroyuki  東京大学, 先端科学技術研究センター, 教授 (10202657)
KAWAMURA Takeshi  東京大学, アイソトープ総合センター, 准教授 (70306835)
INAGAKI Takeshi  東京大学, 先端科学技術研究センター, 特任准教授 (10507825)
MATSUMURA Yoshihiro  東京大学, 先端科学技術研究センター, 助教 (20375257)
FUJII Hotaka  大阪大学, 微生物研究所, 准教授 (30302665)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords生活習慣病 / エピゲノム / ヒストン修飾酵素
Outline of Final Research Achievements

CISPR-CAS9 system is a recently developed gene editing method and its applied method, namely, engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) is beneficial to identify specific nucleotides or proteins that specifically interact to the specific gene loci. In the current study, using these methods, we showed that distal enhancer of β1 adrenergic receptor gene Adrb1, whose transcription is induced upon adrenergic stimulation and whose gene product is important in the activation of brown fat, interacts with Adrb1 promoter via long-lange chromatin looping: deletion of E1 enhancer blunted β-AR induced gene transcription and higher order chromatin structure changes.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (40 results)

All 2016 2015 2014 Other

All Journal Article (8 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 8 results,  Open Access: 8 results,  Acknowledgement Compliant: 5 results) Presentation (30 results) (of which Int'l Joint Research: 5 results,  Invited: 13 results) Book (1 results) Remarks (1 results)

  • [Journal Article] The KDM3A-KLF2-IRF4 axis maintains myeloma cell survival2016

    • Author(s)
      Ohguchi H, Hideshima T, Bhasin MK, Gorgun GT, Santo L, Cea M, Mimura N, Suzuki R, Tai Y-T, Carrasco RD, Raje N, Richardson PG, Harigae H, Sanda T, Sakai J, Anderson KC
    • Journal Title

      Nature Communications

      Volume: 7 Issue: 1 Pages: 10258-10258

    • DOI

      10.1038/ncomms10258

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] JMJD1A is a signal-sensing scaffold that regulates acute chromatin dynamics via SWI/SNF association for thermogenesis2015

    • Author(s)
      Abe Y, Rozqie R, Matsumura Y, Kawamura T, Nakaki R, Tsurutani Y, Tanimura-Inagaki K, Shiono A, Magoori K, Nakamura K, Ogi S, Kajimura S, Kimura H, Tanaka T, Fukami K, Osborne TF, Kodama T, Aburatani H, Inagaki T, Sakai J.
    • Journal Title

      Nat Commun

      Volume: 6 Issue: 1 Pages: 1-14

    • DOI

      10.1038/ncomms8052

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transcriptome Analysis of K-877 (a Novel Selective PPARα Modulator (SPPARMα))-Regulated Genes in Primary Human Hepatocytes and the Mouse Liver2015

    • Author(s)
      Raza-Iqbal S, Tanaka T, Anai M, Inagaki T, Matsumura Y, Ikeda K, Taguchi A, Gonzalez FJ, Sakai J, Kodama T.
    • Journal Title

      Journal of Atherosclerosis and Thrombosis

      Volume: 22 Issue: 8 Pages: 754-772

    • DOI

      10.5551/jat.28720

    • NAID

      130005095007

    • ISSN
      1340-3478, 1880-3873
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Analysis of the subcellular localization of the human histone methyltransferase SETDB12015

    • Author(s)
      Tachibana K, Gotoh E, Kawamata N, Ishimoto K, Uchihara Y, Iwanari H, Sugiyama A, Kawamura T, Mochizuki Y, Tanaka T, Sakai J, Hamakubo T, Kodama T, Doi T.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 465 Issue: 4 Pages: 725-731

    • DOI

      10.1016/j.bbrc.2015.08.065

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] H3K4/H3K9me3 Bivalent Chromatin Domains Targeted by Lineage-Specific DNA Methylation Pauses Adipocyte Differentiation.2015

    • Author(s)
      Matsumura, Y., Nakaki, R., Inagaki, T., Yoshida, A., Kano, Y., Kimura, H., Tanaka, T., Tsutsumi, S., Nakao, M., Doi, T., Fukami, K., Osborne, TF., Kodama, T., Aburatani, H., Sakai, J.
    • Journal Title

      Molecular Cell

      Volume: 60 Issue: 4 Pages: 584-596

    • DOI

      10.1016/j.molcel.2015.10.025

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The FBXL10/KDM2B Scaffolding Protein Associates with Novel Polycomb Repressive Complex-1 to Regulate Adipogenesis2015

    • Author(s)
      Inagaki T, Iwasaki S, Matsumura Y, Kawamura T, Tanaka T, Abe Y, Yamasaki A, Tsurutani Y, Yoshida A, Chikaoka Y, Nakamura K, Magoori K, Nakaki R, Osborne TF, Fukami K, Aburatani H, Kodama T, Sakai J.
    • Journal Title

      J Biol Chem

      Volume: 290 Pages: 4163-4177

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] PPARβ/δactivation of CD300a controls intestinal immunity.2014

    • Author(s)
      Tanaka T, Tahara-Hanaoka, Mabekura T, Ikeda K, Jiang S, Tsutsumi S, Inagaki T, Magoori T, Higurashi H, Takahashi H, Tachibana K, Tsurutani Y, Raza S, Anai M , Minami T, Wada Y, Yokote K, Doi T, Hamakubo T, Auwerx J, Gonzalez J, Nakajima A, Aburatani H, Naito H, Shibuya A, Kodama T, Sakai J.
    • Journal Title

      Scientific Reports

      Volume: 4 Pages: 4-4

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Direct Evidence for Pitavastatin Induced Chromatin Structure Change in the KLF4 Gene in Endothelial Cells.2014

    • Author(s)
      Maejima T, Inoue T, Kanki Y, Kohro T, Li G, Ohta Y, Kimura H, Kobayashi M, Taguchi A, Tsutsumi S, Iwanari H, Yamamoto S, Aruga H, Dong S, Stevens JF, Poh HM, Yamamoto K, Kawamura T, Mimura I,
    • Journal Title

      PLoS One.

      Volume: 9 Issue: 5 Pages: e96005-e96005

    • DOI

      10.1371/journal.pone.0096005

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] H3K4/H3K9me3 Bivalent Chromatin Domains Targeted by Lineage-Specific DNA Methylation Pauses Adipogenesis.2016

    • Author(s)
      Yoshihiro Matsumura, Ryo Nakaki, Takeshi Inagaki, Ayano Yoshida, Yuka Kano, Hiroshi Kimura, Toshiya Tanaka, Shuichi Tsutsumi, Mitsuyoshi Nakao, Takefumi Doi, Kiyoko Fukami, Timothy F. Osborne, Tatsuhiko Kodama, Hiroyuki Aburatani, and Juro Sakai
    • Organizer
      The Keystone Symposia -Chromatin and Epigenetics
    • Place of Presentation
      Whistler, Canada
    • Year and Date
      2016-03-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Metabolic regulation of Adipocyte differentiation by isocitrate dehydrogenease and Jmjc histone2015

    • Author(s)
      酒井寿郎, Eko F.Ariyant, 曽我朋義, 稲垣毅, 松村欣宏
    • Organizer
      第38回日本分子生物学会年会・第88回生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 細胞系譜特異的DNAメチル化により形成されるH3K4/H3K9me3クロマチンドメインは脂肪細胞分化を停止させる2015

    • Author(s)
      松村欣宏, 吉田文乃, 仲木竜, 鹿野優佳, 稲垣毅, 油谷浩幸, 酒井寿郎
    • Organizer
      第38回日本分子生物学会年会・第88回生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] SETDY Regulates C/EBP alpha Transcription during Adipogenesis2015

    • Author(s)
      馬郡健太, 山﨑あゆむ, 大木翔太郎, 松村欣宏, 稲垣毅, 酒井寿郎
    • Organizer
      第38回日本分子生物学会年会・第88回生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] Metabolic Regulation of Histone Demethylation and Glycolysis Gene Expression by Isocitrate Dehydrogenase 3 during Adipogenesis.2015

    • Author(s)
      Eko Fuji Ariyanto, Yoshihiro Matsumura, Tomoyoshi Soga, Takeshi Inagaki and Juro Sakai
    • Organizer
      第38回日本分子生物学会年会・第88回生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒストンメチル化酵素SETDB1のユビキチン化修飾2015

    • Author(s)
      鹿野優佳, 松村欣宏, 吉田文乃, 柳茂, 稲垣毅, 酒井寿郎
    • Organizer
      第38回日本分子生物学会年会・第88回生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] Functional Analysis of the Transcriptional Regulation by Enhancers of the Thermogenesis Related Genes2015

    • Author(s)
      小山一貴, 阿部陽平, 松村欣宏, 稲垣毅, 酒井寿郎
    • Organizer
      第38回日本分子生物学会年会・第88回生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] JMJD1A is a Mediator of Long-Range Genomic Interactions to Regulate Metabolic Gene Expression2015

    • Author(s)
      Takeshi Inagaki, Yohei Abe, Royhan Rozqie, Yoshihiro Matsumura, Takeshi Kawamura, Tatsuhiko Kodama, Hiroyuki Aburatani, Juro Sakai
    • Organizer
      Keystone Symposia Conference;Diabetes
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of Higher-order Chromatin Structure during Thermogenesis in Brown Adipocytes2015

    • Author(s)
      Takeshi Inagaki, Yohei Abe, Royhan Rozqie, Yoshihiro Matsumura, Shingo Kajimura, Juro Sakai
    • Organizer
      The 46th NIPS International Symposium
    • Place of Presentation
      Nagoya
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] PKA dependent phospho-switch on JMJD1A regulates higher-order chromatin dynamics via SWI/SNF and PPARγ association for thermogenesis.2015

    • Author(s)
      Yohei Abe, Royhan Rozqie, Yoshihiro Matsumura, Takeshi Kawamura, Ryo Nakaki, Shotaro Ogi, Toshiya Tanaka, Tatsuhiko Kodama, Hiroyuki Aburatani, Takeshi Inagaki, Juro Sakai
    • Organizer
      EMBO Conference on Nuclear Receptors
    • Place of Presentation
      Ajaccio, Corsica, France
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] JMJD1A Regulates Metabolic Gene Expression by Mediating Long-Range Genomic Interactions in Response to β-Adrenergic Stimulation under Cold Environment2015

    • Author(s)
      Inagaki, T., Abe, Y., Rozqie, R., Matsumura, Y., Kawamura, T., Nakaki, R., Kodama, T., Aburatani, H., Sakai, J.
    • Organizer
      THE 40th NAITO CONFERENCE ON Epigenetics-From Histone Code to Therapeutic Strategy
    • Place of Presentation
      Sapporo
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] Lineage-specific gene-body DNA methylation and non-canonical H3K4/H3K9me3 bivalent chromatin domains maintain adipocyte lineage2015

    • Author(s)
      Yoshihiro Matsumura, Ayano Yoshida, Ryo Nakaki, Kenichi Wakabayashi, Takeshi Inagaki, Kiyoko Fukami, Hiroyuki Aburatani, Juro Sakai
    • Organizer
      THE 40th NAITO CONFERENCE ON Epigenetics-From Histone Code to Therapeutic Strategy
    • Place of Presentation
      Sapporo
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] β-Adrenergic induced phosphorylation of JMJD1A drives energy expenditure via SWI/SNF chromatin remodeler in Brown Adipocytes2015

    • Author(s)
      Sakai, J.
    • Organizer
      The 47th Annual Scientific Meeting of the Japan Atherosclerosis Society
    • Place of Presentation
      Sendai
    • Year and Date
      2015-07-10
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] アディポバイオロジーを制御するヒストン脱メチル化酵素JMJD1Aの修飾シグナル2015

    • Author(s)
      稲垣毅, 阿部陽平, Rozqie Royhan, 松村欣宏, 梶村真吾, 児玉龍彦, 油谷浩幸, 酒井寿郎
    • Organizer
      第58回日本糖尿病学会年次学術集会
    • Place of Presentation
      下関
    • Year and Date
      2015-05-22
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] A Role of Isocitrate Dehydrogenase 3 in Regulation of Epigenome and Transcription during Adipogenesis2015

    • Author(s)
      Juro Sakai, Yoshihiro Matsumura, Ariyanto Fuji Eko, Takeshi Inagaki
    • Organizer
      12th Asian Congress of Nutrirtion
    • Place of Presentation
      Yokohama
    • Year and Date
      2015-05-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 核内受容体PPARγとシグナル感知脱メチル化酵素によるエネルギー代謝制御機構2015

    • Author(s)
      酒井寿郎
    • Organizer
      第88回内分泌学会総会
    • Place of Presentation
      東京
    • Year and Date
      2015-04-25
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 脂肪細胞における新規エピゲノム制御機構2015

    • Author(s)
      稲垣毅, 阿部陽平, Rozqie Royhan, 松村欣宏, 川村猛, 仲木竜, 鶴谷悠也, 谷村恭子, 田中十志也, 児玉龍彦, 油谷浩幸, 酒井寿郎
    • Organizer
      第52回日本臨床分子医学会学術集会
    • Place of Presentation
      京都
    • Year and Date
      2015-04-11
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Lineage-specific gene-body DNA methylation recruits SETDB1 to form non-canonical bivalent chromatin domains that inhibit Cebpa and Pparg expressions and adipogenesis2015

    • Author(s)
      Yoshihiro Matsumura, Ayano Yoshida, Ryo Nakaki, Ken-ichi Wakabayashi, Takeshi Inagaki, Kiyoko Fukami, Hiroyuki Aburatani, Juro Saka
    • Organizer
      DEUEL Conference on Lipids
    • Place of Presentation
      Monterey, California, USA
    • Year and Date
      2015-03-04
    • Related Report
      2014 Research-status Report
  • [Presentation] PKA dependent phospho-switch on JMJD1A regulates higher-order chromatin dynamics via SWI/SNF association for thermogenesis2015

    • Author(s)
      Yohei Abe, Royhan Rozqie, Yoshihiro Matsumura, Takeshi Kawamura, Ryo Nakaki, Yuya Tsurutani, Kyoko Tanimura-Inagaki, Akira Shiono, Kenta Magoori, Kanako Nakamura, Shingo Kajimura, Hiroshi Kimura, Toshiya Tanaka, Kiyoko Fukami, Timothy F. Osborne, Tatsuhiko Kodama, Hiroyuki Aburatani, Takeshi Inagaki, Juro Sakai
    • Organizer
      DEUEL Conference on Lipids
    • Place of Presentation
      Monterey, California, USA
    • Year and Date
      2015-03-04
    • Related Report
      2014 Research-status Report
  • [Presentation] FBXL10/KDM2B Regulates 3T3-L1 Adipogenesis via PRC1-dependent Transcriptional Repression.2015

    • Author(s)
      Takeshi Inagaki, Satoshi Iwasaki, Yoshihiro Matsumura, Yohei Abe, Timothy F. Osborne, Hiroyuki Aburatani, and Juro Sakai
    • Organizer
      DEUEL Conference on lipids
    • Place of Presentation
      Monterey, CA, USA
    • Year and Date
      2015-03-04
    • Related Report
      2014 Research-status Report
  • [Presentation] PKA-dependent Association of Histone Demethylase JMJD1A with SWI/SNF and PPARγ Alters Chromatin Dynamics and Thermogenesis in Brown Adipocytes2014

    • Author(s)
      Juro Sakai
    • Organizer
      The 37th Annual Meeting of the Molecular Biology Society of Japan
    • Place of Presentation
      Pacifico Yokohama, Yokohama
    • Year and Date
      2014-11-26
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Role of Isocitrate Dehydrogenase 3 in Regulation of Epigenome and Transcription during Adipogenesis2014

    • Author(s)
      Ariyanto, E.F., Matsumura, Y., Soga, T., Inagaki, T., Sakai, J.
    • Organizer
      The 37th Annual Meeting of the Molecular Biology Society of Japan
    • Place of Presentation
      Pacifico Yokohama, Yokohama
    • Year and Date
      2014-11-26
    • Related Report
      2014 Research-status Report
  • [Presentation] SETDB1は新規のクロマチンドメインを形成することでCebpαの転写と脂肪細胞分化を制御する2014

    • Author(s)
      松村欣宏,吉田文乃,仲木竜,鹿野優佳,若林賢一,稲垣毅,深見希代子,油谷浩幸,酒井寿郎
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、横浜市
    • Year and Date
      2014-11-26
    • Related Report
      2014 Research-status Report
  • [Presentation] ヒストンメチル化酵素SETDB1のユビキチン化修飾2014

    • Author(s)
      鹿野優佳,松村欣宏,吉田文乃,稲垣毅,酒井寿郎
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、横浜市
    • Year and Date
      2014-11-26
    • Related Report
      2014 Research-status Report
  • [Presentation] JMJD1A のリン酸化スイッチは SWI/SNF および PPARg と複合体を形成しクロマチンの高次構造を変化させる2014

    • Author(s)
      阿部 陽平、Royhan Rozqie、松村 欣宏、川村 猛、仲木 竜、鶴谷 悠也、稲垣 (谷村) 恭子、塩野 陽、馬郡 健太、田中 十志也、児玉 龍彦、油谷 浩幸、稲垣 毅、酒井 寿郎
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、横浜市
    • Year and Date
      2014-11-26
    • Related Report
      2014 Research-status Report
  • [Presentation] Chromatin Regulation via SWI/SNF and PPARγ Association in Brown Adipocytes2014

    • Author(s)
      Juro Sakai
    • Organizer
      The 87th Annual Meeting of the Japanese Biochemical Society
    • Place of Presentation
      Kyoto International Conference Center, Kyoto
    • Year and Date
      2014-10-18
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Metabolic and environmental regulations of JmjC histone demethylases.2014

    • Author(s)
      Juro Sakai
    • Organizer
      The 14th Annual Meeting of the Protein Science Society of Japan
    • Place of Presentation
      Workpia Yokohama, Yokohama
    • Year and Date
      2014-06-27
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 交感神経刺激による脂肪燃焼と肥満におけるエピゲノム変化の役割2014

    • Author(s)
      酒井寿郎
    • Organizer
      第87回日本内分泌学会学術総会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2014-04-24
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] PKA-dependent phosphorylation of JMJD1A drives energy expenditure through Higher-Order Chromatin Regulation via SWI/SNF and PPARγ Association in Brown Adipocytes2014

    • Author(s)
      Juro Sakai
    • Organizer
      The 18th International Vascular Biology Meeting
    • Place of Presentation
      Miyakomesse, Kyoto
    • Year and Date
      2014-04-15
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Metabolic and environmental regulations of JmjC histone demethylases.2014

    • Author(s)
      Juro Sakai
    • Organizer
      The 18th International Vascular Biology Meeting
    • Place of Presentation
      Miyakomesse, Kyoto
    • Year and Date
      2014-04-14
    • Related Report
      2014 Research-status Report
  • [Book] エピジェネティクスの産業応用2014

    • Author(s)
      酒井寿郎
    • Total Pages
      12
    • Publisher
      シーエムシー出版
    • Related Report
      2014 Research-status Report
  • [Remarks] 東京大学先端科学技術研究センター酒井研究室/原著論文

    • URL

      http://www.mm.rcast.u-tokyo.ac.jp/publications/pubs.html

    • Related Report
      2015 Annual Research Report 2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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