Elucidation of fetal wound healing mechanisms in cardiac fibroblasts and its therapeutic implications of myocardial infarction.
| Project/Area Number |
26560399
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied health science
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| Research Institution | Aomori University of Health and Welfare |
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Principal Investigator |
KON ATSUSHI 青森県立保健大学, 健康科学部, 教授 (60271798)
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| Co-Investigator(Renkei-kenkyūsha) |
SAWAMURA DAISUKE 弘前大学, 大学院医学研究科, 教授 (60196334)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 胎仔創傷治癒機構 / 心臓線維芽細胞 / 心筋梗塞 / 遺伝子発現 / 発生 / 胎仔創傷治癒 |
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| Outline of Final Research Achievements |
In this study, we analyzed the specific gene expressed in fetal wound healing (FWH) in mouse cardiac fibroblast cells characterized by without scar formation. By using DNA microarray analysis, anti-fibrosis related genes including Has2, Tm, and Mmp genes, and morphogenesis related genes (Foxo, Hox, and Pax) highly expressed in FWH, whereas the expression of these genes down-regulated in adult wound healing (AWH) with scar formation. Down regulation of Pax gene expression are down regulated by methylation on histone H3 domain, whereas downregulation of other genes were induced by DNA methylation of each promoter region, respectively. Finally, we injected each gene into myocardial infarction tissue of adult mice. As the result, each gene did not induce FWH characterized perfect scarless reaction, suggesting alone administration of each gene was not effective, and cocktail therapy, the combination of multiple genes, are necessary of inducing of FWH.
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Report
(4 results)
Research Products
(2 results)
