| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Animal studies have shown that regular exercise prevents atherosclerosis. Autophagy has been functionally linked to homeostasis in organs. Here, we determined if regular exercise-induced autophagy activation is sufficient to mitigate high-fat diet-induced atherosclerosis. Male C57BL/6J mice were randomly assigned into Sedentary (Sed) and (Ex) group. Mice in exercise group were performed voluntary wheel-running exercise for 16 weeks. Mice in both groups were fed 45% high-fat diet. After exercise training periods, aorta tissue was harvested and analyzed autophagy proteins by western blot. We showed that 16 weeks regular exercise prevented high-fat diet-induced obesity and atherosclerosis. Autophagy flux (i.e., LC3-II protein) in Sed mice was greater than Ex mice. These results suggest that exercise training may regulate atherosclerotic plaques formation by autophagy.
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