Development of antibacterial agents of periodontal pathogens : a basic study

• Project/Area Number: 26560405
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied health science
• Research Institution: Matsumoto Dental University
• Principal Investigator: HIRAOKA Yukihiro 松本歯科大学, 総合歯科医学研究所, 教授 (20097512)
• Co-Investigator(Kenkyū-buntansha): 中道 裕子 松本歯科大学, 総合歯科医学研究所, 講師 (20350829) ; 山下 照仁 松本歯科大学, 総合歯科医学研究所, 准教授 (90302893)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: SOD / 歯周病原菌 / 金属酵素 / 部位特異的変異 / 金属選択性 / 酵素

Outline of Final Research Achievements

The anaerobe P. gingivalis is one of the organisms most strongly associated with chronic adult periodontitis. Superoxide dismutases (SOD), which are　essential for aerobic life, playing an important protective role against　oxidative stress. This research is a basic study aiming at the development of inhibitors of P. gingivalis SOD (Pg SOD) by structural features. We focused on Leu-72, Leu-76 and Gly-155, which are located near the active-site metal, they were constructed a mutant enzyme by the site-directed mutagenesis and discussed its chemical properties. Based on the results of this study, we herein conclude that the integrity of Leu-72, Leu-76 and Gly-155 are a necessary requisite for the metal-tolerant activity of Pg SOD.

Research Products

• [Journal Article] Effect of substituting Trp for Leu at position 72 on the structure of Porphyromonas gingivalis superoxide dismutase. (2014)
  • Author(s): Miho Komachiya, Shinya Mizoue , Masashi Mihara, Masaki Osawa, Yuichiro Kikuchi, Setsuko Uematsu, Kaname Hirai, Yuichiro Okubo, Akihiro Kuroiwa, Kazuhiro Yamada, Fumiyuki Yamakura and B.Yukihiro Hiraoka
  • Journal Title: 松本歯学 Volume: 40 Pages: 19-25
  • NAID: 120005487655
  • Peer Reviewed / Open Access
• [Journal Article] Contribution of the amino acid residues located near the active site metal to the metal-specific activity of Porphyromonas gingivalis SOD induced by a double mutation of Leu72Trp and Leu76Phe. (2014)
  • Author(s): Masashi Mihara, Miho Komachiya, Shinya Mizoue , Masaki Osawa, Setsuko Uematsu, Yuichiro Kikuchi, Yuichiro Okubo, Kaname Hirai, Akihiro Kuroiwa, Kazuhiro Yamada, Fumiyuki Yamakura and B.Yukihiro Hiraoka
  • Journal Title: 松本歯学 Volume: 40 Pages: 26-34
  • Peer Reviewed / Open Access