In vitro selection of novel functional RNAs and peptides by means of natural RNA library

Research Project

Project/Area Number	26560429
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Biomolecular chemistry
Research Institution	The University of Tokyo
Principal Investigator	Katoh Takayuki 東京大学, 大学院理学系研究科(理学部), 助教 (90567760)
Project Period (FY)	2014-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Keywords	機能性ペプチド / 機能性RNA / in vitroセレクション / アゴニスト / マイクロRNA
Outline of Final Research Achievements	We developed a new method for screening novel functional RNAs and peptides that specifically bind to target molecules in cell. Here, we prepared a total RNA library derived from various human tissues, then mixed them with folate molecules covalently immobilized on magnetic beads to isolate folate-binding RNAs. The RNA library was also subjected to in vitro translation to prepare a peptide library, which was then used to screen Keap1 binding peptides by means of mRNA display.

Report

(5 results)