In vitro selection of novel functional RNAs and peptides by means of natural RNA library
Project/Area Number |
26560429
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Biomolecular chemistry
|
Research Institution | The University of Tokyo |
Principal Investigator |
Katoh Takayuki 東京大学, 大学院理学系研究科(理学部), 助教 (90567760)
|
Project Period (FY) |
2014-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
|
Keywords | 機能性ペプチド / 機能性RNA / in vitroセレクション / アゴニスト / マイクロRNA |
Outline of Final Research Achievements |
We developed a new method for screening novel functional RNAs and peptides that specifically bind to target molecules in cell. Here, we prepared a total RNA library derived from various human tissues, then mixed them with folate molecules covalently immobilized on magnetic beads to isolate folate-binding RNAs. The RNA library was also subjected to in vitro translation to prepare a peptide library, which was then used to screen Keap1 binding peptides by means of mRNA display.
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Report
(5 results)
Research Products
(4 results)