Engineering of type III polyketide synthase by deletion and addition of residues
| Project/Area Number |
26560435
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry
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| Research Institution | University of Toyama |
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Principal Investigator |
MORITA HIROYUKI 富山大学, 和漢医薬学総合研究所, 教授 (20416663)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 酵素工学 / ポリケタイド合成酵素 / X線結晶構造解析 / 立体構造解析 |
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| Outline of Final Research Achievements |
In order to modify the function of type III polyketide synthase, we carried out deletion mutagenesis studies on octaketide synthase (OKS), which catalyzes sequential condensations of eight molecules of malonyl-CoA to produce SEK4/SEK4b. The deletion mutagenesis studies revealed that the loss of Varine351, lining the active site cavity of OKS, results in specializing its starter substrate specificity in fatty acyl CoAs. Furthermore, a crystal structure analysis of the mutant enzyme suggested that an expansion of the active-site cavity near catalytic residue, cysteine, specialized the substrate specificity of the mutant in the fatty acyl CoAs.
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Report
(3 results)
Research Products
(18 results)
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[Journal Article] Structural basis for the formation of acylalkylpyrones from two β-ketoacyl units by the fungal type III polyketide synthase CsyB2015
Author(s)
Mori, T., Yang, D., Matsui, T, Hashimoto, M., Morita, H., Fujii, I., Abe, I.
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Journal Title
J. Biol. Chem.
Volume: 290
Issue: 8
Pages: 5214-5225
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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