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Molecular recognition in the interfacial environment induced by lipid membranes

Research Project

Project/Area Number 26560436
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomolecular chemistry
Research InstitutionOsaka University

Principal Investigator

Matsuoka Shigeru  大阪大学, 理学(系)研究科(研究院), 特任准教授 (60456184)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords脂肪酸 / 側方相互作用 / リポソーム / 熱測定 / 脂質-タンパク質相互作用 / 脂質二重膜 / 分子認識
Outline of Final Research Achievements

The cell membrane, a separator for the interior of cells from the outside environment, is formed by lipids which are relative insoluble in water. Its lipid component is not uniform and changes in each small structural unit of the micro - nanometer scale to play key roles in the signal transduction and the mass transport across the cell membrane. Lipophilic compounds in vivo are not dissolvable in water, and are adsorbed on the surface of hydrophobic sites such as the cell membrane. Therefore, lipophilic compounds reacts with biomolecules in membrane bound structures rather than in aqueous solution structures. In this study, we succeeded in the quantification of the effect of the membrane lipid composition on the reaction between lipophilic compounds and soluble protein as free energy by using isothermal titration calorimetry.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (14 results)

All 2015 2014

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 3 results,  Open Access: 1 results) Presentation (9 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results)

  • [Journal Article] Phosphatidylcholine bearing 6,6-dideuterated oleic acid: A useful solid-state 2H NMR probe for investigating membrane properties.2015

    • Author(s)
      Jin Cui, Sebastien Lethu, Tomokazu Yasuda, Shigeru Matsuoka, Nobuaki Matsumori, Fuminori Sato, Michio Murata
    • Journal Title

      Bioorg. Med. Chem.

      Volume: 25 Issue: 2 Pages: 203-206

    • DOI

      10.1016/j.bmcl.2014.11.072

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 脂肪酸結合タンパク質の分子認識2015

    • Author(s)
      松岡 茂
    • Journal Title

      BIO Clinica

      Volume: 30 Pages: 77-82

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Centerband-only analysis of rotor-unsynchronized spin-echo for measurement of lipid 31P chemical shift anisotropy.2015

    • Author(s)
      Yuichi Umegawa, Toshiyuki Yamaguchi, Michio Murata, Shigeru Matsuoka*
    • Journal Title

      Magn. Reson. Chem.

      Volume: 53 Issue: 7 Pages: 514-519

    • DOI

      10.1002/mrc.4247

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Molecular Dynamics Simulations of Heart-type Fatty Acid-binding Protein in Apo and Holo Forms, and Hydration Structure Analyses in the Binding Cavity.2015

    • Author(s)
      Daisuke Matsuoka, Shigeru Sugiyama, Michio Murata, Shigeru Matsuoka*
    • Journal Title

      J. Phys. Chem. B

      Volume: 119 Issue: 1 Pages: 114-127

    • DOI

      10.1021/jp510384f

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Water-mediated recognition of simple alkyl chains by heart-ype fatty acid-binding protein.2015

    • Author(s)
      Shigeru Matsuoka, Shigeru Sugiyama, Daisuke Matsuoka, Mika Hirose, Sebastien Lethu, Hikaru Ano, Toshiaki Hara, Osamu Ichihara, S. Roy Kimura, Satoshi Murakami, Hanako Ishida, Eiichi Mizohata, Tsuyoshi Inoue, Michio Murata
    • Journal Title

      Angew. Chem. Int. Ed.

      Volume: 54 Issue: 5 Pages: 1508-1511

    • DOI

      10.1002/anie.201409830

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Isotope-labeled phospholipids for examining domain-specific order profiles and compositional distribution by solid state NMR2015

    • Author(s)
      M. Murata, T. Yasuda, J. Cui, M. Kinoshita, S. Matsuoka, N. Matsumori
    • Organizer
      Pacifichem 2015, Symposium on “Function, Chemistry, and Signaling of Glycolipids and Phospholipids”
    • Place of Presentation
      Honolulu
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Solid-state NMR investigations of small-molecule binding sites2015

    • Author(s)
      S. Matsuoka
    • Organizer
      Symposium on “Fragment-based Lead Discovery”
    • Place of Presentation
      Honolulu
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Head group-selective interaction of cations and sugars with phospholipids in multiple component bilayer systems2015

    • Author(s)
      S. Matsuoka
    • Organizer
      Pacifichem 2015, Symposium on “Small Molecule Interactions in Biomembranes”
    • Place of Presentation
      Honolulu
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Interplay between Internal Water Molecules and Bound Fatty Acid Molecule in FABP3 Binding Cavity2015

    • Author(s)
      松岳大輔, 松岡茂、杉山成、村田道雄
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学角間キャンパス
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 脂質-タンパク質間相互作用の解明を目指したバクテリオロドプシン脂質アナログの立体選択的合成2015

    • Author(s)
      Jin Cui、川竹悟史、梅川雄一、Sèbastian Lethu 、松岡 茂、佐藤文憲、松森信明、村田道雄
    • Organizer
      第57回天然有機化合物討論会
    • Place of Presentation
      神奈川県民ホール
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 脂質-脂質分子間相互作用の固体NMR研究2015

    • Author(s)
      松岡 茂
    • Organizer
      難治疾患完治療開発のための革新的イメージ脂質生物学の確立・研究成果中間発表会
    • Place of Presentation
      金沢医科大学
    • Year and Date
      2015-04-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] 化学シフト異方性測定に基づくバクテリオロドプシン-周辺脂質の親和性評価2014

    • Author(s)
      梅川雄一, 川竹悟史, 松岡茂, 村田道雄
    • Organizer
      第53回NMR討論会
    • Place of Presentation
      大阪大学コンベンションセンター(大阪)
    • Year and Date
      2014-11-04 – 2014-11-06
    • Related Report
      2014 Research-status Report
  • [Presentation] 多成分脂質膜のCSA測定2014

    • Author(s)
      松岡茂, 梅川雄一, 村田道雄
    • Organizer
      第53回NMR討論会
    • Place of Presentation
      大阪大学コンベンションセンター(大阪)
    • Year and Date
      2014-11-04 – 2014-11-06
    • Related Report
      2014 Research-status Report
  • [Presentation] 生物固体試料の局所構造解析2014

    • Author(s)
      松岡茂
    • Organizer
      新学術領域研究「天然物ケミカルバイオロジー~分子標的と活性制御~」・第6回若手研究者ワークショップ「機器分析のケミカルバイオロジーへの応用」
    • Place of Presentation
      千里ライフサイエンスセンター(大阪)
    • Year and Date
      2014-10-29
    • Related Report
      2014 Research-status Report
    • Invited

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Published: 2014-04-04   Modified: 2017-05-10  

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