Molecular recognition in the interfacial environment induced by lipid membranes

• Project/Area Number: 26560436
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: Osaka University
• Principal Investigator: Matsuoka Shigeru 大阪大学, 理学(系)研究科(研究院), 特任准教授 (60456184)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 脂肪酸 / 側方相互作用 / リポソーム / 熱測定 / 脂質－タンパク質相互作用 / 脂質二重膜 / 分子認識

Outline of Final Research Achievements

The cell membrane, a separator for the interior of cells from the outside environment, is formed by lipids which are relative insoluble in water. Its lipid component is not uniform and changes in　each small structural unit of the micro - nanometer scale to play key roles in the signal transduction and the mass transport across the cell membrane. Lipophilic compounds in vivo are not dissolvable in water, and are adsorbed on the surface of hydrophobic sites such as the cell membrane. Therefore, lipophilic compounds reacts with biomolecules in membrane bound structures rather than in aqueous solution structures. In this study, we succeeded in the quantification of the effect of the membrane lipid composition on the reaction between lipophilic compounds and soluble protein as free energy by using isothermal titration calorimetry.

Research Products

• [Journal Article] Phosphatidylcholine bearing 6,6-dideuterated oleic acid: A useful solid-state 2H NMR probe for investigating membrane properties. (2015)
  • Author(s): Jin Cui, Sebastien Lethu, Tomokazu Yasuda, Shigeru Matsuoka, Nobuaki Matsumori, Fuminori Sato, Michio Murata
  • Journal Title: Bioorg. Med. Chem. Volume: 25 Issue: 2 Pages: 203-206
  • DOI: 10.1016/j.bmcl.2014.11.072
  • Peer Reviewed
• [Journal Article] 脂肪酸結合タンパク質の分子認識 (2015)
  • Author(s): 松岡 茂
  • Journal Title: BIO Clinica Volume: 30 Pages: 77-82
• [Journal Article] Centerband-only analysis of rotor-unsynchronized spin-echo for measurement of lipid 31P chemical shift anisotropy. (2015)
  • Author(s): Yuichi Umegawa, Toshiyuki Yamaguchi, Michio Murata, Shigeru Matsuoka*
  • Journal Title: Magn. Reson. Chem. Volume: 53 Issue: 7 Pages: 514-519
  • DOI: 10.1002/mrc.4247
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Molecular Dynamics Simulations of Heart-type Fatty Acid-binding Protein in Apo and Holo Forms, and Hydration Structure Analyses in the Binding Cavity. (2015)
  • Author(s): Daisuke Matsuoka, Shigeru Sugiyama, Michio Murata, Shigeru Matsuoka*
  • Journal Title: J. Phys. Chem. B Volume: 119 Issue: 1 Pages: 114-127
  • DOI: 10.1021/jp510384f
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Water-mediated recognition of simple alkyl chains by heart-ype fatty acid-binding protein. (2015)
  • Author(s): Shigeru Matsuoka, Shigeru Sugiyama, Daisuke Matsuoka, Mika Hirose, Sebastien Lethu, Hikaru Ano, Toshiaki Hara, Osamu Ichihara, S. Roy Kimura, Satoshi Murakami, Hanako Ishida, Eiichi Mizohata, Tsuyoshi Inoue, Michio Murata
  • Journal Title: Angew. Chem. Int. Ed. Volume: 54 Issue: 5 Pages: 1508-1511
  • DOI: 10.1002/anie.201409830
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Isotope-labeled phospholipids for examining domain-specific order profiles and compositional distribution by solid state NMR (2015)
  • Author(s): M. Murata, T. Yasuda, J. Cui, M. Kinoshita, S. Matsuoka, N. Matsumori
  • Organizer: Pacifichem 2015, Symposium on “Function, Chemistry, and Signaling of Glycolipids and Phospholipids”
  • Place of Presentation: Honolulu
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Solid-state NMR investigations of small-molecule binding sites (2015)
  • Author(s): S. Matsuoka
  • Organizer: Symposium on “Fragment-based Lead Discovery”
  • Place of Presentation: Honolulu
  • Year and Date: 2015-12-15
  • Int'l Joint Research / Invited
• [Presentation] Head group-selective interaction of cations and sugars with phospholipids in multiple component bilayer systems (2015)
  • Author(s): S. Matsuoka
  • Organizer: Pacifichem 2015, Symposium on “Small Molecule Interactions in Biomembranes”
  • Place of Presentation: Honolulu
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Interplay between Internal Water Molecules and Bound Fatty Acid Molecule in FABP3 Binding Cavity (2015)
  • Author(s): 松岳大輔, 松岡茂、杉山成、村田道雄
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学角間キャンパス
  • Year and Date: 2015-09-13
• [Presentation] 脂質-タンパク質間相互作用の解明を目指したバクテリオロドプシン脂質アナログの立体選択的合成 (2015)
  • Author(s): Jin Cui、川竹悟史、梅川雄一、Sèbastian Lethu 、松岡 茂、佐藤文憲、松森信明、村田道雄
  • Organizer: 第57回天然有機化合物討論会
  • Place of Presentation: 神奈川県民ホール
  • Year and Date: 2015-09-09
• [Presentation] 脂質-脂質分子間相互作用の固体NMR研究 (2015)
  • Author(s): 松岡 茂
  • Organizer: 難治疾患完治療開発のための革新的イメージ脂質生物学の確立・研究成果中間発表会
  • Place of Presentation: 金沢医科大学
  • Year and Date: 2015-04-14
• [Presentation] 化学シフト異方性測定に基づくバクテリオロドプシン－周辺脂質の親和性評価 (2014)
  • Author(s): 梅川雄一, 川竹悟史, 松岡茂, 村田道雄
  • Organizer: 第53回NMR討論会
  • Place of Presentation: 大阪大学コンベンションセンター(大阪)
  • Year and Date: 2014-11-04 – 2014-11-06
• [Presentation] 多成分脂質膜のCSA測定 (2014)
  • Author(s): 松岡茂, 梅川雄一, 村田道雄
  • Organizer: 第53回NMR討論会
  • Place of Presentation: 大阪大学コンベンションセンター(大阪)
  • Year and Date: 2014-11-04 – 2014-11-06
• [Presentation] 生物固体試料の局所構造解析 (2014)
  • Author(s): 松岡茂
  • Organizer: 新学術領域研究「天然物ケミカルバイオロジー～分子標的と活性制御～」・第6回若手研究者ワークショップ「機器分析のケミカルバイオロジーへの応用」
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)
  • Year and Date: 2014-10-29
  • Invited