| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
i-Motif is one of a non-B DNA structure, which is believed to construct in the C-rich regions in DNA. Since the no i-motif stabilizing compounds have been reported, no information about the function of i-motif has been obtained. In this research we aimed to develop i-mtoif selective interacting molecules, i.e., i-motif ligands.
Among our synthetic libraries together with the compounds suggested by the in silico docking studies, we started ca. forty compounds as candidates for i-motif ligands. With the evaluations by FRET melting analysis, CD spectra analysis, and EMAS experiments, we found the compound which selectively stabilize i-motifs derived from telomere, c-kit, c-myc and bcl-2, respectively. Stabilizing ability of the ligand is high, and delta Tm valu was obtained at 20 °C.
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