| Project/Area Number |
26560448
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Osaka University |
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Principal Investigator |
Murata Michio 大阪大学, 理学(系)研究科(研究院), 教授 (40183652)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 配座解析 / 分子運動 / 疎水性相互作用 / 脂質二重膜 / 脂肪酸 / 結合タンパク質 / 脂肪酸結合タンパク質 / 疎水分子の水和 / 膜タンパク質 / 周辺脂質 / 分子認識機構 |
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| Outline of Final Research Achievements |
Cell membranes consist of lipids and proteins, where lipid-lipid and lipid-protein interactions in the aqueous environment plays essential roles in various cellular events. Hydrophobic interactions are known to be essential for the molecular recognitions responsible for stabilization of membrane proteins. In this project, the role of water molecules in lipid-protein interactions was investigated using calorimetry, X-ray crystallography, and molecular simulations. The results clearly show that water molecules in the binding pocket of fatty acid is stabilized by hydrogen-bond network between water and sidechains and thus, changes in sidechain residues do not lead to the significant movement of the bound ligand and water molecules.
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