Development of a fast photo-stimulation device

• Project/Area Number: 26560456
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Basic / Social brain science
• Research Institution: Saitama University
• Principal Investigator: NAKAI Junichi 埼玉大学, 理工学研究科, 教授 (80237198)
• Co-Investigator(Renkei-kenkyūsha): GENGYO-ANDO Keiko 埼玉大学, 理工学研究科, 特任教授 (40221741)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 光遺伝学 / KTNスキャナー / 多光子励起 / 高速走査

Outline of Final Research Achievements

Recently, optogenetics is getting popular for studying neuronal circuits. In this research, we developed a photostimulation device that used a fast XY scanner with potassium tantalate niobate (KTN) crystal. World-fast two photon excitation of fluorescein could be achieved with this newly developed photostimulation device with KTN scanner. Scan speed of the KTN scanner is more than 10 times faster than that of the galvano scanner. This research will advance brain science and natural science in which optical technique is more important.

Research Products

• [Journal Article] JSAP1/JIP3 and JLP regulate kinesin-1-dependent axonal transport to prevent neuronal degeneration. (2015)
  • Author(s): Sato T, Ishikawa M, Mochizuki M, Ohta M, Ohkura M, Nakai J, Takamatsu N, Yoshioka K
  • Journal Title: Cell Death Differ Volume: - Issue: 8 Pages: 1260-1274
  • DOI: 10.1038/cdd.2014.207
  • Peer Reviewed
• [Journal Article] RING finger protein 121 facilitates the degradation and membrane localization of voltage-gated sodium channels (2015)
  • Author(s): Ogino, K., Low, S.E., Yamada, K., Saint-Amant, L., Zhou, W., Muto, A., Asakawa, K., Nakai, J., Kawakami, K., Kuwada, J.Y., and Hirata, H.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 112 Issue: 9 Pages: 2859-2864
  • DOI: 10.1073/pnas.1414002112
  • Peer Reviewed
• [Journal Article] Rational design of a high-affinity, fast, red calcium indicator R-CaMP2. (2015)
  • Author(s): Inoue M, Takeuchi A, Horigane S, Ohkura M, Gengyo-Ando K, Fujii H, Kamijo S, Takemoto-Kimura S, Kano M, Nakai J, Kitamura K, Bito H
  • Journal Title: Nat Methods Volume: 12 Issue: 1 Pages: 64-70
  • DOI: 10.1038/nmeth.3185
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mitochondrial Ca2+ uptake by the voltage-dependent anion channel 2 regulates cardiac rhythmicity. (2014)
  • Author(s): Shimizu H, Schredelseker J, Huang J, Lu K, Naghdi S, Lu F, Franklin S, Fiji HD, Wang K, Zhu H, Tian C, Lin B, Nakano H, Ehrlich A, Nakai J, Stieg AZ, Gimzewski JK, Nakano A, Goldhaber JI, Vondriska TM, Hajnoczky G, Kwon O, Chen JN.
  • Journal Title: Elife Volume: 4 Pages: 1-20
  • DOI: 10.7554/elife.04801
  • Peer Reviewed / Open Access
• [Journal Article] Reward-timing-dependent bidirectional modulation of cortical microcircuits during optical single-neuron operant conditioning. (2014)
  • Author(s): Hira R et al.
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 5551-5551
  • DOI: 10.1038/ncomms6551
  • Peer Reviewed / Open Access
• [Journal Article] Excitation propagation in three-dimensional engineered hearts using decellularized extracellular matrix. (2014)
  • Author(s): Yasui H, Lee JK, Yoshida A, Yokoyama T, Nakanishi H, Miwa K, Naito AT, Oka T, Akazawa H, Nakai J, Miyagawa S, Sawa Y, Sakata Y, Komuro I
  • Journal Title: Biomaterials Volume: 35 Issue: 27 Pages: 7839-7850
  • DOI: 10.1016/j.biomaterials.2014.05.080
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Toll-like receptor 9 protects non-immune cells from stress by modulating mitochondrial ATP synthesis through the inhibition of SERCA2. (2014)
  • Author(s): Shintani Y, Drexler HC, Kioka H, Terracciano CM, Coppen SR, Imamura H, Akao M, Nakai J, Wheeler AP, Higo S, Nakayama H, Takashima S, Yashiro K, Suzuki K.
  • Journal Title: EMBO Reports Volume: 15 Issue: 4 Pages: 438-445
  • DOI: 10.1002/embr.201337945
  • Peer Reviewed / Open Access
• [Journal Article] Large-scale imaging of subcellular calcium dynamics of cortical neurons with G-CaMP6-actin (2014)
  • Author(s): 小林千晃, 他
  • Journal Title: Neuroreport Volume: 25(7) Issue: 7 Pages: 501-506
  • DOI: 10.1097/wnr.0000000000000126
  • Peer Reviewed
• [Presentation] A new transgenic mouse model revealed a role of astrocytic calcium in vivo (2015)
  • Author(s): Tanaka M, Shih P-Y, Gomi H, Yoshida T, Nakai J, Ando R, Furuichi T, Mikoshiba K, Samyanov A, Itohara S.
  • Organizer: 第88回日本薬理学会年会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-03-18 – 2015-03-20
• [Presentation] Rational design of a high-affinity, fast, red calcium indicator R-CaMP2. (2015)
  • Author(s): 井上昌俊、竹内敦也、堀金慎一郎、大倉正道、安藤恵子、藤井哉、上條諭志、竹本―木村さやか、狩野方伸、中井淳一、喜多村和郎、尾藤晴彦
  • Organizer: 第88回日本薬理学会年会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-03-18 – 2015-03-20
• [Presentation] 個体(線虫) レベルでの超多並列刺激応答モニターシステム (2014)
  • Author(s): 藤泰一、高橋尚也、安藤恵子、大倉正道、中井淳一、西垣功一
  • Organizer: 第３７回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 低分子量GTPase RAB-11の新規結合因子REI-1/REI-2は受精後のRAB-11再局在化を制御する (2014)
  • Author(s): 坂口愛沙、佐藤美由紀、安藤恵子、佐藤克哉、中井淳一、佐藤健
  • Organizer: 第３７回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Imaging Deep-Layer Neuronal Activity in Mouse Barrel Cortex using 1040-nm Excitation of a Red Fluorescent Protein Calcium Indicator (2014)
  • Author(s): Carta S, Liu W, Sych Y, Voigt FF, Chen JL, Schneider B, Ohkura M, Nakai J, Helmchen F
  • Organizer: Neuroscience 2014
  • Place of Presentation: Washington, DC USA
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] A comparative study of the two-photon performances of GCaMPs and GECOs (2014)
  • Author(s): Podor B, Zhao Y, Wu J, Hu Y-L, Ohkura M, Nakai J, Campbell R, Croll R, Fine A
  • Organizer: Neuroscience 2014
  • Place of Presentation: Washington, DC USA
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] Neuronal mechanisms of decision making in C. elegans olfactory behavior revealed by a highly integrated microscope system. (2014)
  • Author(s): Tanimoto Y, Yamazoe A, Fujita K, Kawazoe Y, Miyanishi Y, Yamazaki SJ, Gengyo-Ando K, Nakai J, Fei X, Iwasaki Y, Hashimoto K, Kimura K
  • Organizer: 第34回日本神経科学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-09 – 2014-11-13
• [Presentation] Single-neuron operant conditioning by two-photon imaging induces reward-timing-dependent modulation in cortical microcircuit. (2014)
  • Author(s): Hira R, Ohkubo F, Mazamizu Y, Ohkura M, Nakai J, Okada T, Matsuzaki M
  • Organizer: 第34回日本神経科学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-09 – 2014-11-13
• [Presentation] The top-down circuit for sensory perception in the cerebral cortex of the mouse. (2014)
  • Author(s): Manita S, Suzuki T, Homma C, Matsumoto T, Odagawa M, Yamada K, Ota K, Matsubara C, Inutsuka A, Sato M, Ohkura M, Yamanaka A, Yanagawa Y, Nakai J, Hayashi Y, Larkum ME, Murayama M
  • Organizer: 第34回日本神経科学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-09 – 2014-11-13
• [Presentation] Imaging of the neural circuit activities during retrieval of behavioral program for active avoidance in zebrafish. (2014)
  • Author(s): Kakinuma H, Aoki R, Aoki T, Yamazaki M, Shiraki T, Takahoko M, Eizumi K, Koide T, Yoshihara Y, Nakai J, Kawakami K, Okamoto H
  • Organizer: 第34回日本神経科学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-09 – 2014-11-13
• [Presentation] Visualization of cerebral cortical calcium dynamics by a transgenic mouse that express G-CaMP7 in neurons and glia. (2014)
  • Author(s): Monai H, Ohkura M, Tanaka M, Itohara S, Nakai J, Hirase H
  • Organizer: 第34回日本神経科学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-09 – 2014-11-13
• [Presentation] JSAP1 and JLP regulate kinesin-1-dependent axonal transport to prevent neuronal degeneration. (2014)
  • Author(s): Sato T, Ohkura M, Nakai J, Takamatsu N, Yoshioka K
  • Organizer: 第34回日本神経科学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-09 – 2014-11-13
• [Presentation] 蛍光カルシウムプローブ G-CaMP による in vivo カルシウムイメージング (2014)
  • Author(s): 中井淳一、大倉正道, 安藤恵子
  • Organizer: 第５８回日本薬学会関東支部大会
  • Place of Presentation: 昭和大学
  • Year and Date: 2014-10-04
• [Presentation] ホヤ幼生の遊泳運動神経回路の構造と生理機能の解析 (2014)
  • Author(s): 堀江健生, 大倉正道, 日下部岳広, 中井淳一, 中川将司
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 東北大学
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 細胞触覚センサのための小型蛍光観察システムの開発 (2014)
  • Author(s): 民山浩輔, 清水正宏, 宮坂恒太, 小椋利彦, 中井淳一, 大倉正道, 細田耕
  • Organizer: 第32回日本ロボット学会学術講演会
  • Place of Presentation: 九州産業大学
  • Year and Date: 2014-09-04 – 2014-09-06
• [Book] Optical Imaging of Neocortical Dynamics, Neuromethods, vol. 85 (2014)
  • Author(s): Takata N., Shinohara Y., Ohkura M., Mishima T., Nakai J. & Hirase H.
  • Total Pages: 296
  • Publisher: Springer Science Business Media
• [Remarks] 埼玉大学脳末梢科学研究センター
  • URL: http://subsi.saitama-u.ac.jp/
• [Patent(Industrial Property Rights)] カルシウム指示遺伝子 (2014)
  • Inventor(s): 尾藤晴彦, 井上昌俊, 竹内敦也, 中井淳一, 大倉正道
  • Industrial Property Rights Holder: 尾藤晴彦, 井上昌俊, 竹内敦也, 中井淳一, 大倉正道
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-06-11