| Project/Area Number |
26560459
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Basic / Social brain science
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
Tsukada Yuki 名古屋大学, 理学研究科, 助教 (80580000)
|
|---|
| Research Collaborator |
MORI Ikue
NAKANO Shunji
HASHIMOTO Koichi
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 顕微鏡制御 / C. elegans / カルシウムイメージング / 画像処理 / 画像解析 / 実データ解析 / 探索行動 / 神経 |
|---|
| Outline of Final Research Achievements |
We have developed a tracking system that automatically tracks microorganisms navigating freely on a small dish. The system succeeded in tracking nematodes acting freely on the temperature gradient under a high magnification objective lens. Fluorescence calcium probes were expressed in specific neurons and neural activity was measured with this system in noninvasive manner under a freely behaving condition. To analyze the time-lapse imaging data obtained from this tracking system, an image analysis program was developed. Using these tools, we analyzed behavioral factors and neural activities related to thermotaxis behavior. Our research helps to understand how activity of interneurons contributes to regulate behavior during exploratory navigation.
|
