monitoring activity of neuronal micro domain using high speed tracking system

• Project/Area Number: 26560459
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Basic / Social brain science
• Research Institution: Nagoya University
• Principal Investigator: Tsukada Yuki 名古屋大学, 理学研究科, 助教 (80580000)
• Research Collaborator: MORI Ikue ; NAKANO Shunji ; HASHIMOTO Koichi
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 顕微鏡制御 / C. elegans / カルシウムイメージング / 画像処理 / 画像解析 / 実データ解析 / 探索行動 / 神経

Outline of Final Research Achievements

We have developed a tracking system that automatically tracks microorganisms navigating freely on a small dish. The system succeeded in tracking nematodes acting freely on the temperature gradient under a high magnification objective lens. Fluorescence calcium probes were expressed in specific neurons and neural activity was measured with this system in noninvasive manner under a freely behaving condition. To analyze the time-lapse imaging data obtained from this tracking system, an image analysis program was developed. Using these tools, we analyzed behavioral factors and neural activities related to thermotaxis behavior. Our research helps to understand how activity of interneurons contributes to regulate behavior during exploratory navigation.

Research Products

• [Journal Article] Reconstruction of spatial thermal gradient encoded in thermosensory neuron AFD in Caenorhabditis elegans. (2016)
  • Author(s): Tsukada, Y., Yamao, M., Honda, N., Shimowada, T., Ohnishi, N., Kuhara, A., Ishii, S., Mori, I.
  • Journal Title: J Neurosci. Volume: 36 Issue: 9 Pages: 2571-2581
  • DOI: 10.1523/jneurosci.2837-15.2016
  • NAID: 120006360790
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Identifi cation of RIA interneuron-mediated behavioral components essential for thermotaxis (2016)
  • Author(s): Linghuan Xu, Yuki Tsukada, Muneki Ikeda, Shunji Nakano, Ikue Mor
  • Organizer: CeNeuro2016 C. elegans Topic Meeting: NEURONAL DEVELOPMENT, SYNAPTIC FUNCTION & BEHAVIOR
  • Place of Presentation: 名古屋大学豊田講堂
  • Year and Date: 2016-07-27
  • Int'l Joint Research
• [Presentation] Integration of thermal and chemical information in C. elegans (2016)
  • Author(s): Amame Kano, Amane Kano, Yuki Tsukada, Ikue Mori
  • Organizer: CeNeuro2016 C. elegans Topic Meeting: NEURONAL DEVELOPMENT, SYNAPTIC FUNCTION & BEHAVIOR
  • Place of Presentation: 名古屋大学豊田講堂
  • Year and Date: 2016-07-27
  • Int'l Joint Research
• [Presentation] Switching among exploitation and exploration behavioral modes in the nematode C. elegans (2016)
  • Author(s): Hironori J. Matsuyama, Yuki Tsukada, Satomi Tsukamoto, Masataka Yamao, Honda Naoki, Shin Ishii, and Ikue Mori
  • Organizer: CeNeuro2016 C. elegans Topic Meeting: NEURONAL DEVELOPMENT, SYNAPTIC FUNCTION & BEHAVIOR
  • Place of Presentation: 名古屋大学豊田講堂
  • Year and Date: 2016-07-27
  • Int'l Joint Research
• [Presentation] Toward functional connectomics at synaptic level (2016)
  • Author(s): Yuki Tsukada, Ikue Mori
  • Organizer: CeNeuro2016 C. elegans Topic Meeting: NEURONAL DEVELOPMENT, SYNAPTIC FUNCTION & BEHAVIOR
  • Place of Presentation: 名古屋大学豊田講堂
  • Year and Date: 2016-07-27
  • Int'l Joint Research
• [Presentation] Quantification and reconstruction of thermosensory neuronal processing in C. elegans (2016)
  • Author(s): Yuki TSUKADA
  • Organizer: NIG International Symposium 2016 Force, Information and Dynamics: X factors shaping living systems
  • Place of Presentation: 東京大学生産技術研究所
  • Year and Date: 2016-01-10
  • Int'l Joint Research / Invited
• [Presentation] 動画像からの行動解析－線虫を例に－ (2016)
  • Author(s): 塚田 祐基
  • Organizer: 昆虫デザイン研究会 家畜化過程の理解とポスト家畜化
  • Place of Presentation: 岡崎コンファレンスセンター
  • Invited
• [Presentation] Neural encoding of thermotaxis behavior in C. elegans (2015)
  • Author(s): Yuki TSUKADA, Naoki Honda, Masataka Yamao, Shin Ishii, Ikue MORI
  • Organizer: Neuro2015
  • Place of Presentation: 神戸国際会議場・神戸国際展示場
  • Year and Date: 2015-07-29
  • Int'l Joint Research
• [Presentation] High-speed, high-magnification tracking system for monitoring interneuronal activity of freely moving C. elegans (2014)
  • Author(s): Yuki TSUKADA, Xianfeng FEI, Chen MIN, Koichi HASHIMOTO, Ikue MORI
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Year and Date: 2014-09-09
• [Presentation] 画像解析と顕微鏡制御を駆使した動く対象へのイメージングとオプトジェネティクス (2014)
  • Author(s): 塚田 祐基
  • Organizer: 第6回光塾
  • Place of Presentation: 未来ICT研究所（兵庫県）
  • Year and Date: 2014-09-06
  • Invited
• [Book] ImageJではじめる生物画像解析 (2016)
  • Author(s): 三浦 耕太, 塚田 祐基（編著）
  • Total Pages: 295
  • Publisher: 学研メディカル秀潤社
• [Book] "Optogenetics in Caenorhabditis elegans." in Optogenetics -Light-Sensing Proteins and Their Applications (2015)
  • Author(s): Yuki TSUKADA and Ikue MORI
  • Publisher: Springer