Visualization and quantification of fluxes of glucose metabolic pathways in brain
| Project/Area Number |
26560469
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Brain biometrics
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| Research Institution | Keio University |
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Principal Investigator |
Sugiura Yuki 慶應義塾大学, 医学部(信濃町), 講師 (30590202)
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| Co-Investigator(Renkei-kenkyūsha) |
Norio Takata 慶應義塾大学, 医学部, 特任講師 (50415212)
Kenji Tanata 慶應義塾大学, 医学部, 准教授 (30329700)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 脳代謝 / イメージング質量分析 / fMRI / グルコース代謝 |
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| Outline of Final Research Achievements |
In response to a stimuli, neurons and astrocytes alter their metabolic pathways to fit their elevated energy demands. For example, it is known that a stimulation to an animal increase the amount of glucose consumption in the brain region specific manner. To understand molecular mechanism of this phenomenon, a molecular imaging approach is effective. In this research, we developed the novel flux analysis using stable isotopes in combination with MALDI- imaging mass spectrometry, as well as quantitative metabolomics. Those lead us to answer the questions including “during a neural stimulation, in what anatomical regions of the brain, is which metabolic derangement occurring?”. We finally identified a novel metabolic mediator shuttled between neurons and astrocytes up on the stimulation.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Cystathionine beta-synthase and PGRMC1 as CO sensors.2016
Author(s)
Kabe Y, Yamamoto T, Kajimura M, Sugiura Y, Koike I, Ohmura M, Nakamura T, Tokumoto Y, Tsugawa H, Handa H, Kobayashi T, and Suematsu M.
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Journal Title
Free Radical Biology & Medicine
Volume: 99
Pages: 333-344
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Therapeutic hypothermia achieves neuroprotection via a decrease in acetylcholine with a concurrent increase in carnitine in the neonatal hypoxia-ischemia2015
Author(s)
2.Takenouchi T, Sugiura Y, Morikawa T, Nakanishi T, Nagahata Y, Sugioka T, Honda K, Kubo A, Hishiki T, Matsuura T, Hoshino T, Takahashi T, Suematsu M, and Kajimura M
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Journal Title
Journal of Cerebral Blood Flow & Metabolism
Volume: 35
Issue: 5
Pages: 1-12
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Non-neuronal acetylcholine as an endogenous regulator of proliferation and differentiation of Lgr5-positive stem cells in mice2014
Author(s)
Toshio Takahashi, Hiroe Ohnishi, Yuki Sugiura, Kurara Honda, Makoto Suematsu, Takashi Kawasaki, Tomonori Deguchi, Takeshi Fujii, Kaoru Orihashi, Yoshitaka Hippo, Takehiro Watanabe, Tohro Yamagaki and Shunsuke Yuba
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Journal Title
the FEBS J
Volume: 281
Issue: 20
Pages: 4672-4690
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] DHA-PC and PSD-95 decrease after loss of synaptophysin and before neuronal loss in patients with Alzheimer's disease.2014
Author(s)
Yuki D, Sugiura Y, Zaima N, Akatsu H, Takei S, Yao I, Maesako M, Kinoshita A, Yamamoto T, Kon R, Sugiyama K, Setou M.
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Journal Title
Sci Rep
Volume: -
Issue: 1
Pages: 7130-7130
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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