In vivo Ca/kinase imaging in freely moving mouse using micro-endoscope

• Project/Area Number: 26560470
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Brain biometrics
• Research Institution: Foundation for Biomedical Research and Innovation (2015); 公益財団法人大阪バイオサイエンス研究所 (2014)
• Principal Investigator: Funabiki Kazuo 公益財団法人先端医療振興財団, その他部局等, 研究員 (00301234)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: in vivo imaging / action selection / basal ganglia / system neuroscience / PKA / ERK / FRET / キナーゼ / 神経活動 / Ca imaging / free moving / 細胞内情報伝達系 / 大脳基底核

Outline of Final Research Achievements

We made a micro-endoscope system enabling FRET imaging in freely moving animals. By combining it with the mutants in which PKA or ERK FRET biosensor is expressed specifically in the striatal projection neurons of either the direct or the indirect pathway of the basal ganglia circuitry, we explored in vivo dynamics of PKA or ERK response of either pathway against several behavioral paradigms. We revealed that PKA and ERK of the direct and indirect pathways, in principal, show activation in a reciprocal manner. We further revealed that these dynamic shifts of PKA or ERK activities between direct and indirect pathways occur in a more natural situation, such as the action selection in male's mating behavior. Furthermore, we found that the behavioral threshold for the significant activation of ERK of the indirect pathway neurons is quite low, which may indicate that the indirect pathway would be more important in action selection occurring in daily life.

Research Products

• [Journal Article] Htr2a-Expressing Cells in the Central Amygdala Control the Hierarchy between Innate and Learned Fear. (2015)
  • Author(s): Isosaka T, Matsuo T, Yamaguchi T, Funabiki K, Nakanishi S, Kobayakawa R, Kobayakawa K.
  • Journal Title: Cell Volume: 163 Issue: 5 Pages: 1153-1164
  • DOI: 10.1016/j.cell.2015.10.047
  • Peer Reviewed / Open Access
• [Journal Article] Role of PKA signaling in D2 receptor-expressing neurons in the core of the nucleus accumbens in aversive learning (2015)
  • Author(s): Yamaguchi T, Goto A, Nakahara I, Yawata S, Hikida T, Matsuda M, Funabiki K, Nakanishi S
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: VOL. 112 Issue: 36 Pages: 11383-11388
  • DOI: 10.1073/pnas.1514731112
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Circuit-dependent striatal PKA and ERK signaling underlies rapid behavioral shift in mating reaction of male mice. (2015)
  • Author(s): Goto A, Nakahara I, Yamaguchi T, Kamioka Y, Sumiyama K, Matsuda M, Nakanishi S, Funabiki K
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: １１２ Issue: 21 Pages: 6718-6723
  • DOI: 10.1073/pnas.1507121112
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Minimal conductance-based model of auditory coincidence detector neurons. (2015)
  • Author(s): Ashida G, Funabiki K, Kretzberg J.
  • Journal Title: PLoS One Volume: 10 Issue: 4 Pages: e0122796-e0122796
  • DOI: 10.1371/journal.pone.0122796
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Bedside evaluation of smooth pursuit eye movements in acute sensory stroke patients. (2015)
  • Author(s): Johkura K, Kawabata Y, Amano Y, Kudo Y, Murata H, Kirimura S, Funabiki K.
  • Journal Title: J Neurol Sci. Volume: 348 Issue: 1-2 Pages: 269-271
  • DOI: 10.1016/j.jns.2014.12.013
  • Peer Reviewed
• [Journal Article] One-third of vertiginous episodes during the follow-up period are caused by benign paroxysmal positional vertigo in patients with Meniere's disease (2014)
  • Author(s): Taura, A. Funabiki, K. Ohgita, H. Ogino, E. Torii, H. Matsunaga, M. Ito, J.
  • Journal Title: Acta Otolaryngol Volume: 134 Issue: 11 Pages: 1140-5
  • DOI: 10.3109/00016489.2014.936624
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Enhanced stability of hippocampal place representation caused by reduced magnesium block of NMDA receptors in the dentate gyrus (2014)
  • Author(s): Yuichiro Hayashi, Yoko Nabeshima, Katsunori Kobayashi, Tsuyoshi Miyakawa, Koichi Tanda, Keizo Takao, Hidenori Suzuki, Eisaku Esumi, Shigeru Noguchi, Yukiko Matsuda, Toshikuni Sasaoka, Tesuo Noda, Jun-ichi Miyazaki, Masayoshi Mishina, Kazuo Funabiki and Yo-ichi Nabeshima
  • Journal Title: Molecular Brain Volume: 7 Issue: 1 Pages: 44-44
  • DOI: 10.1186/1756-6606-7-44
  • Peer Reviewed / Open Access
• [Journal Article] Aversive behavior induced by optogenetic inactivation of ventral tegmental area dopamine neuronsis mediated by dopamine D2 receptors in the nucleus accumbens. (2014)
  • Author(s): Danjo T, Yoshimi K, Funabiki K, Yawata S, *Nakanishi S
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 111 Issue: 17 Pages: 6455-60
  • DOI: 10.1073/pnas.1404323111
  • Peer Reviewed
• [Journal Article] Role of granule-cell transmission in memory trace of cerebellum-dependent optokinetic motor learning (2014)
  • Author(s): Wada N, Funabiki K, *Nakanishi S.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 111 Issue: 14 Pages: 5373-5378
  • DOI: 10.1073/pnas.1402546111
  • Peer Reviewed