| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
The aim of this study was to provide materials and techniques to allow easy and non-invasive measurement of intracellular single molecule behavior. To achieve this, we attempted to introduce: 1) ability to specifically bind molecules of interest, using tag protein technologies that can form a covalent bond between tag-fusion proteins and small molecule ligands; and 2) cell permeability, using cell permeable peptides to introduce extremely stable and bright fluorescent nanocrystal quantum dots. We successfully introduced the former ability, but we aborted the latter, due to drawbacks, such as transduction efficiency. However, we enabled highly efficient transduction of quantum dots by electroporation, without noticeable cytotoxicity. With this approach, we successfully tracked intracellular movement of myosin motor proteins.
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