| Project/Area Number |
26600052
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nanobioscience
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IINO Ryota 自然科学研究機構, 岡崎統合バイオサイエンスセンター, 教授 (70403003)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 一分子計測 / 膜タンパク質 / 高速原子間力顕微鏡 / １分子イメージング・ナノ計測 |
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| Outline of Final Research Achievements |
We prepared nano-pore arrays with a diameter of 100nm on glass and silicon substrate using electron-beam lithography and focused ion beam. Also we succeeded in sealing the nano pores with lipid bilayers. Purple membranes in which two dimensional crystals of bacteriorhodopsin are embedded were used as a test sample and the individual bacteriorhodopsin molecules were resolved on the nano pore with high-speed AFM. We also developed a method to conveniently prepare the nano pores on a silicone elastomer using small beads with a diameter of 100nm as a template. We observed ion-channel membrane protein KcsA on a supported lipid bilayer and visualized individual KcsA tetramers and theirs diffusion dynamics in the membrane. As the next step, we will reconstitute KcsA molecules into the membrane on the nano pore and reveal structural dynamics of KcsA..
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