Optical detection of single DNA molecules using a silicon nanopore for readout of sequence information
| Project/Area Number |
26600055
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nanobioscience
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
IWASAKI Wataru 東京大学, 大学院理学系研究科, 准教授 (50545019)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ナノポアセンシング / DNA / 蛍光計測 / プラズモン / １分子計測 / ナノ光学 / ナノ流体工学 / ナノデバイス / 生体生命情報学 / バイオセンシング / ナノオプティクス |
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| Outline of Final Research Achievements |
The challenge in highly accurate identification of each nucleotide is slowing and stabilizing DNA translocation velocity. For this purpose, DNA conformation and drift-diffusion motion immediately before and after translocation should be investigated. In this study, an optical microscopic method with 100-nm and 0.1-ms resolution is developed to observe DNA coiling immediately after translocation and its motion. Characteristic DNA translocation dynamics caused by the electric field in the vicinity of nanopore exit is obtained and the mechanism is discussed in detail. To achieve higher spatial resolution aiming to single-molecule sequencing, the possibility of combination with STED and plasmonic nanopore is also demonstrated.
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Report
(3 results)
Research Products
(12 results)
