Development of Assembled Cell Model by Using Liposome-Liposome Adhesions

• Project/Area Number: 26600066
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nano/Microsystems
• Research Institution: Kanagawa Academy of Science and Technology
• Principal Investigator: OSAKI TOSHIHISA 公益財団法人神奈川科学技術アカデミー, 人工細胞膜システムグループ, 研究員 (50533650)
• Co-Investigator(Renkei-kenkyūsha): MIURA Shigenori 東京大学, 生産技術研究所, 研究員 (70511244)
• Research Collaborator: HAMANO Hiroshige
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 生体機能模倣 / 脂質 / 生体材料（リポソーム） / マイクロ・ナノデバイス / 合成生物学

Outline of Final Research Achievements

In former works, single liposomes, capsule forms of a lipid bilayer, were applied for studies on single cell models, playing a role of a compartment to isolate a cytoplasm model. Here we focused on the tissue structure rather than the individual cells, and developed an assembled cell model by taking advantage of the adhesion of multiple liposomes; we further aim to reproduce and understand the concerted functions expressed by the mass of cells in living tissues in the future work. Based on our previous works, which enabled the formation of cell-sized liposomes in array format, liposome-liposome assembly was implemented by using streptavidin-biotin connections.

Research Products

• [Journal Article] 4．人工細胞膜作製とシングルイオンチャネル計測 (2015)
  • Author(s): 神谷厚輝、大崎寿久、竹内昌治
  • Journal Title: Electrochemistry Volume: 83 Issue: 12 Pages: 1096-1100
  • DOI: 10.5796/electrochemistry.83.1096
  • NAID: 130005112142
  • ISSN: 1344-3542, 2186-2451
  • Year and Date: 2015-12-05
  • Peer Reviewed
• [Presentation] Microfluidics For Biosensing And Healthcare Applications (2016)
  • Author(s): Toshihisa Osaki
  • Organizer: ISPlasma2016/IC-PLANTS2016
  • Place of Presentation: 名古屋大学（愛知・名古屋）
  • Year and Date: 2016-03-08
  • Int'l Joint Research / Invited
• [Presentation] Non-Spherical Liposomes Formed By Molecular Structure And Deposition Micropattern (2015)
  • Author(s): Toshihisa Osaki, Koki Kamiya, and Shoji Takeuchi
  • Organizer: The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: 慶州（韓国）
  • Year and Date: 2015-10-28
  • Int'l Joint Research
• [Presentation] 生体膜デバイスの創薬・センサ応用 (2015)
  • Author(s): 大崎寿久
  • Organizer: 第14回国際バイオテクノロジー展
  • Place of Presentation: 東京ビッグサイト（東京・江東区）
  • Year and Date: 2015-05-13
• [Presentation] LIPOSOME ARRANGEMENT CONNECTED WITH AVIDIN-BIOTIN COMPLEX FOR CONSTRUCTING FUNCTIONAL SYNTHETIC TISSUE (2015)
  • Author(s): Hiroshige Hamano, Toshihisa Osaki, and Shoji Takeuchi
  • Organizer: The 28th IEEE International Conference on Micro Electro Mechanical Systems
  • Place of Presentation: エストリル（ポルトガル）
  • Year and Date: 2015-01-20
• [Presentation] Artificial Cell Membrane Devices for Pharmaceutical Applications (2014)
  • Author(s): Toshihisa Osaki, Koki Kamiya, Kosuke Shibasaki, and Shoji Takeuchi
  • Organizer: 8th International Symposium on Nanomedicine
  • Place of Presentation: 愛媛大学（愛媛・松山）
  • Year and Date: 2014-12-05
  • Invited
• [Presentation] MICROFLUIDIC HYDRATION OF DRY LIPID PATTERNS FOR DEVELOPING EPITHELIAL CELL MODEL (2014)
  • Author(s): Hiroshige Hamano, Taishi Tonooka, Toshihisa Osaki, and Shoji Takeuchi
  • Organizer: The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: サンアントニオ（アメリカ合衆国）
  • Year and Date: 2014-10-28
• [Book] Synthetic Biology Volume 1 (2014)
  • Author(s): Toshihisa Osaki, Koki Kamiya, and Shoji Takeuchi
  • Total Pages: 374
  • Publisher: The Royal Society of Chemistry
• [Book] 生体の科学 (2014)
  • Author(s): 外岡大志、大崎寿久、竹内昌治
  • Total Pages: 141
  • Publisher: 医学書院
• [Remarks] 公益財団法人神奈川科学技術アカデミー人工細胞膜システムグループ
  • URL: http://www.newkast.or.jp/innovation/labo/takeuchi_project.html
• [Remarks] Research Gate - Toshihisa Osaki
  • URL: https://www.researchgate.net/profile/Toshihisa_Osaki