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Development of digital counting method using SERS

Research Project

Project/Area Number 26600117
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Optical engineering, Photon science
Research InstitutionOsaka University

Principal Investigator

Ando Jun  大阪大学, 工学研究科, 特任研究員(常勤) (40623369)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords表面増強ラマン散乱 / ラマン分光法 / 金属ナノ粒子
Outline of Final Research Achievements

Surface-enhanced Raman scattering spectroscopy uses metallic nanostructures, which amplifies Raman scattering signal from the molecule existing near the metal surface. Since it can identify molecular species of the sample at high sensitivity, and further provides information of the molecular structure and surrounding environment, it is expected to be a promising analytical method for various research fields. However, the range of its application has been limited, since the precise control of the degree of signal amplification is often challenging. In this research, digital counting was considered to use for the measure of the quantification of SERS signal, instead of the SERS signal intensity. By using it, improvement of the sensitivity of SERS measurement and quantitative analytical capability was examined.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (25 results)

All 2017 2016 2015

All Journal Article (5 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 5 results) Presentation (17 results) (of which Int'l Joint Research: 8 results,  Invited: 5 results) Book (3 results)

  • [Journal Article] Surface-enhanced Raman scattering (SERS) imaging of alkyne-tagged small molecule drug in live cells with endocytosed gold nanoparticles2017

    • Author(s)
      Jun Ando, Takumasa Sekiya, Hiroyuki Yamakoshi, Den Ka, Kosuke Dodo, Mikiko Sodeoka, Satoshi Kawata, Katsumasa Fujita
    • Journal Title

      Proc. of SPIE

      Volume: 10046 Pages: 10046-1

    • DOI

      10.1117/12.2256126

    • Related Report
      2016 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Alkyne-tag SERS screening and identification of small-molecule-binding sites in protein2016

    • Author(s)
      Jun Ando, Miwako Asanuma, Kosuke Dodo, Hiroyuki Yamakoshi, Satoshi Kawata, Katsumasa Fujita, Mikiko Sodeoka
    • Journal Title

      Journal of the American Chemical Society

      Volume: 138 Issue: 42 Pages: 13901-13910

    • DOI

      10.1021/jacs.6b06003

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] High-speed Raman imaging of cellular processes2016

    • Author(s)
      Jun Ando, Almar F Palonpon, Mikiko Sodeoka, Katsumasa Fujita
    • Journal Title

      Current Opinion in Chemical Biology

      Volume: 33 Pages: 16-24

    • DOI

      10.1016/j.cbpa.2016.04.005

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Alkyne-tag Raman imaging of bio-active small molecules in live cells2015

    • Author(s)
      Jun Ando, Almar F. Palonpon, Hiroyuki Yamakoshi, Kosuke Dodo, Satoshi Kawata, Mikiko Sodeoka, Katsumasa Fujita
    • Journal Title

      Proc. SPIE Biophotonics Japan 2015

      Volume: 9792 Pages: 979207-979207

    • DOI

      10.1117/12.2207554

    • Related Report
      2015 Research-status Report
    • Acknowledgement Compliant
  • [Journal Article] Raman spectroscopic detection of bio-active small molecules using alkyne tag2015

    • Author(s)
      Jun Ando, Almar F. Palonpon, Satoshi Kawata, Katsumasa Fujita, Hiroyuki Yamakoshi, Kosuke Dodo, Mikiko Sodeoka
    • Journal Title

      CPMT Symposium Japan 2015 IEEE

      Volume: 1 Pages: 93-96

    • DOI

      10.1109/icsj.2015.7357369

    • Related Report
      2015 Research-status Report
    • Acknowledgement Compliant
  • [Presentation] Surface-enhanced Raman scattering (SERS) imaging of alkyne-tagged small molecule drug in live cells with endocytosed gold nanoparticles2017

    • Author(s)
      Jun Ando, Takumasa Sekiya, Hiroyuki Yamakoshi, Den Ka, Kosuke Dodo, Mikiko Sodeoka, Satoshi Kawata, Katsumasa Fujita
    • Organizer
      SPIE Photonics west BiOS 2017
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2017-01-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生細胞に投与した薬剤のアルキン標識・増強ラマンイメージング2017

    • Author(s)
      安藤 潤, 関谷 拓正, Ka Den, 山越 博幸, どど孝介, 袖岡 幹子, 河田 聡, 藤田 克昌
    • Organizer
      第64回応用物理学会春季学術講演会
    • Place of Presentation
      パシフィコ横浜, 神奈川
    • Related Report
      2016 Annual Research Report
  • [Presentation] SERS imaging of alkyne-tagged small molecule in cells with gold nanoparticles2016

    • Author(s)
      Jun Ando, Takumasa Sekiya, Den Ka, Hiroyuki Yamakoshi, Kosuke Dodo, Mikiko Sodeoka, Satoshi Kawata, Katsumasa Fujita
    • Organizer
      Optics & Photonics Japan 2016
    • Place of Presentation
      筑波大学, 東京
    • Year and Date
      2016-10-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Raman imaging of lipid rafts in an artificial monolayer membrane2016

    • Author(s)
      Jun Ando, Masanao Kinoshita, Jin Cui, Hiroyuki Yamakoshi, Kosuke Dodo, Michio Murata, Mikiko Sodeoka, Katsumasa Fujita
    • Organizer
      Focus on Microscopy 2016
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2016-03-20
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ラマン散乱を利用した細胞分子イメージング2016

    • Author(s)
      安藤潤, 藤田克昌
    • Organizer
      第24回分子複合医薬研究会
    • Place of Presentation
      産業技術総合研究所, 大阪
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ラマン散乱を用いた細胞分析イメージング2016

    • Author(s)
      安藤潤
    • Organizer
      京都バイオ計測センターシンポジウム, 食・ヘルスケアから未病診断への新しいバイオ計測
    • Place of Presentation
      京都バイオ計測センター, 京都
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Rapid SERS imaging of biomolecules using slit-scannning Raman microscopy2016

    • Author(s)
      J. Ando, K. Bando, K. Mochizuki, K. Dodo, H. Yamakoshi, K. Fujita, M. Sodeoka, S. Kawata
    • Organizer
      NFO-14: The 14th International Conference of Near-Field Optics, Nanophotonics and Related Techniques
    • Place of Presentation
      Shizuoka, Japan
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ラマン散乱を用いた生体イメージング2016

    • Author(s)
      安藤潤, 藤田克昌
    • Organizer
      2016年 電子情報通信学会ソサイエティ大会
    • Place of Presentation
      北海道大学, 札幌市
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ラマン散乱による細胞分析イメージング2016

    • Author(s)
      安藤潤, 藤田克昌
    • Organizer
      第36回ポリマー光部品(POC)研究会
    • Place of Presentation
      産業技術総合研究所, 大阪
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] High-resolution Raman imaging of lipid rafts in artificial monolayer membranes2015

    • Author(s)
      Jun Ando, Masanao Kinoshita, Jin Cui, Hiroyuki Yamakoshi, Kosuke Dodo, Katsumasa Fujita, Michio Murata, Mikiko Sodeoka
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Raman spectroscopic detection of bio-active small molecules using alkyne-tag2015

    • Author(s)
      Jun Ando, Almar F. Palonpon, Satoshi Kawata, Katsumasa Fujita, Hiroyuki Yamakoshi, Kosuke Dodo, Mikiko Sodeoka
    • Organizer
      IEEE CPMT Symposium Japan 2015
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2015-11-09
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 3D SERS imaging of intracellular pathways with endocytosed gold nanoparticles2015

    • Author(s)
      Jun Ando, Kazuki Bando, Kentaro Mochizuki, Nicholas I. Smith, Katsumasa Fujita, Satoshi Kawata
    • Organizer
      5th International Conference on Tip-enhanced Raman Spectroscopy
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2015-10-29
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Alkyne-tag Raman imaging of bio-active small molecules in live cells2015

    • Author(s)
      Jun Ando, Almar F. Palonpon, Hiroyuki Yamakoshi, Kosuke Dodo, Satoshi Kawata, Mikiko Sodeoka, Katsumasa Fujita
    • Organizer
      SPIE/OSJ Biophotonics Japan
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2015-10-27
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Hyperspectral Raman imaging of lipid rafts in artificial monolayer membranes2015

    • Author(s)
      Jun Ando, Masanao Kinoshita, Jin Cui, Hiroyuki Yamakoshi, Kosuke Dodo, Katsumasa Fujita, Michio Murata, Mikiko Sodeoka
    • Organizer
      SciX 2015
    • Place of Presentation
      Providence, USA
    • Year and Date
      2015-09-27
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Visualization of lipid rafts in an artificial monolayer membrane by using slit-scanning Raman microscopy2015

    • Author(s)
      Jun Ando, Masanao Kinoshita, Jin Cui, Hiroyuki Yamakoshi, Kosuke Dodo, Katsumasa Fujita, Michio Murata, Mikiko Sodeoka
    • Organizer
      第76回応用物理学会秋季学術講演会
    • Place of Presentation
      名古屋国際会議場, 愛知
    • Year and Date
      2015-09-15
    • Related Report
      2015 Research-status Report
  • [Presentation] アルキン標識を用いた小分子のバイオラマンイメージング2015

    • Author(s)
      安藤 潤
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学, 石川
    • Year and Date
      2015-09-13
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 人工単分子膜中の脂質ラフトの高解像度ラマン分光イメージング2015

    • Author(s)
      安藤 潤, 木下 祥尚, Cui Jin, 山越 博幸, どど 孝介, 藤田 克昌, 村田 道雄, 袖岡 幹子
    • Organizer
      日本分光学会 平成27年度年次講演会
    • Place of Presentation
      東京工業大学, 東京
    • Year and Date
      2015-06-01
    • Related Report
      2015 Research-status Report
  • [Book] 光アライアンス2017年5月号, ラマン分光顕微鏡とライフサイエンス応用2017

    • Author(s)
      安藤潤, 藤田克昌
    • Total Pages
      6
    • Publisher
      日本工業出版(株)
    • Related Report
      2016 Annual Research Report
  • [Book] 機関誌 Vol.9, No.2, ラマン散乱を利用した細胞分子イメージング2016

    • Author(s)
      安藤潤, 袖岡幹子, 藤田克昌
    • Total Pages
      9
    • Publisher
      日本分子イメージング学会
    • Related Report
      2016 Annual Research Report
  • [Book] アルキン標識ラマンイメージング : 生体内の低分子化合物を見る2015

    • Author(s)
      安藤 潤, どど 孝介, 藤田 克昌, 袖岡 幹子
    • Total Pages
      5
    • Publisher
      バイオインダストリー協会 (バイオサイエンスとインダストリー Vol. 73)
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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