Quantitative measurements and controlled designs of firefly bioluminescence with genetic bio-technology
| Project/Area Number |
26610081
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Condensed matter physics I
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HIYAMA Miyabi 東京大学, 物性研究所, 研究員 (90399311)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 生物発光 / 酵素 / ルシフェラーゼ / ルシフェリン / 分光 |
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| Outline of Final Research Achievements |
We studied quantum yields, spectra, and their dependences on temperatures and solvents, on luciferin-luciferase reactions in firefly bioluminescence, by using biosynthetic mutant luciferase enzyme, where charges, polarity, and hydrophobicity/ hydrophilicity of amino acid residues surrounding oxyluciferin are modified. We also measured quantum yields of biosynthetic mutant photo-protein (aequorin) in jellyfish bioluminescence, by revising our quantitative spectrometer to cover 400 nm blue region. Theoretical analyses via time-dependent density-functional calculations, first-principle calculations, and molecular dynamics calculations including water and protein molecules were made.
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Report
(3 results)
Research Products
(44 results)
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[Presentation] Calibration standards and measurement accuracy of absolute electroluminescence and internal properties in multi-junction and arrayed solar cells2016
Author(s)
Masahiro Yoshita, Lin Zhu, Changsu Kim, Toshimitsu Mochizuki, Tetsuya Nakamura, Mitsuru Imaizumi, Shaoqiang Chen, Hidehiro Kubota, Yoshihiko Kanemitsu, and Hidefumi Akiyama
Organizer
SPIE Photonics West San Francisco
Place of Presentation
San Francisco, USA
Year and Date
2016-02-13
Related Report
Invited
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[Presentation] イクオリン生物発光の量子収率測定2015
Author(s)
挾間優治, 東暉舜, 倉田麻貴, 樋山みやび, 吉田正裕, 久保田英博, 井上敏, 秋山英文
Organizer
日本物理学会2015年秋季大会
Place of Presentation
関西大学 千里山キャンパス（大阪府吹田市山手町）
Year and Date
2015-09-16
Related Report
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