| Project/Area Number |
26610081
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Condensed matter physics I
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HIYAMA Miyabi 東京大学, 物性研究所, 研究員 (90399311)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 生物発光 / 酵素 / ルシフェラーゼ / ルシフェリン / 分光 |
|---|
| Outline of Final Research Achievements |
We studied quantum yields, spectra, and their dependences on temperatures and solvents, on luciferin-luciferase reactions in firefly bioluminescence, by using biosynthetic mutant luciferase enzyme, where charges, polarity, and hydrophobicity/ hydrophilicity of amino acid residues surrounding oxyluciferin are modified. We also measured quantum yields of biosynthetic mutant photo-protein (aequorin) in jellyfish bioluminescence, by revising our quantitative spectrometer to cover 400 nm blue region. Theoretical analyses via time-dependent density-functional calculations, first-principle calculations, and molecular dynamics calculations including water and protein molecules were made.
|
