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Developments of the high-frequency ESR system using the micro-cantilever and its application to the detection of protein

Research Project

Project/Area Number 26610104
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Condensed matter physics II
Research InstitutionKobe University

Principal Investigator

Ohta Hitoshi  神戸大学, 分子フォトサイエンス研究センター, 教授 (70194173)

Co-Investigator(Kenkyū-buntansha) 大道 英二  神戸大学, 理学研究科, 准教授 (00323634)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsカンチレバー / 電子スピン共鳴 / 金属タンパク質 / ESR / タンパク質計測 / 高周波 / 高感度 / 高周波数
Outline of Final Research Achievements

We have succeeded in extending the frequency region of piezo-resistive micro-cantilever ESR up to 1.1 THz, which is the world record for such micro-cantilever ESR measurement. Moreover, we have developed a new micro-cantilever ESR system with much higher sensitivity by using the Fabry-Perot interferometer, which enables the detection of the displacement of micro-cantilever with high accuracy. Using this new micro-cantilever ESR system, we have succeeded in observing high frequency ESR of hemin (about 100 ng), which is the active center of hem protein, up to 0.5 THz. Multi-frequency ESR measurements of hemin have enabled us to obtain the zero-field splitting parameters in details.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (26 results)

All 2017 2016 2015 2014

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Acknowledgement Compliant: 5 results) Presentation (19 results) (of which Int'l Joint Research: 7 results,  Invited: 8 results)

  • [Journal Article] Force-detected ESR measurements in a terahertz range up to 0.5 THz and application to hemin2017

    • Author(s)
      Tsubasa Okamoto, Hideyuki Takahashi, Eiji Ohmichi, Hitoshi Ohta
    • Journal Title

      Applied Magnetic Resonance

      Volume: 48 Issue: 5 Pages: 435-444

    • DOI

      10.1007/s00723-017-0872-2

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Wide-dynamic-range cantilever magnetometry using a fiber-optic interferometer and its application to high-frequency electron spin resonance spectroscopy2016

    • Author(s)
      Hideyuki Takahashi, Tsubasa Okamoto, Eiji Ohmichi, and Hitoshi Ohta
    • Journal Title

      Applied Physics Express

      Volume: 9 Issue: 12 Pages: 126701-126701

    • DOI

      10.7567/apex.9.126701

    • NAID

      210000138131

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of Ultrasensitive Terahertz ESR Spectroscopy for Metalloprotein Using a Microcantilever2016

    • Author(s)
      T. Okamoto, H. Takahashi, E. Ohmichi, and H. Ohta
    • Journal Title

      Journal of Infrared, Millimeter, and Terahertz Waves

      Volume: 37 Issue: 12 Pages: 1173-1184

    • DOI

      10.1007/s10762-016-0305-6

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Multi-frequency force-detected electron spin resonance in the millimeter-wave region up to 150 GHz2016

    • Author(s)
      E. Ohmichi, Y. Tokuda, R. Tabuse, D. Tsubokura, T. Okamoto, and H. Ohta
    • Journal Title

      Review of Scientific Instruments

      Volume: 87 Issue: 7

    • DOI

      10.1063/1.4959152

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] High-frequency electron paramagnetic resonance of metal-containing porphyrin compounds using a microcantilever2016

    • Author(s)
      Eiji Ohmichi, Tsubasa Okamoto, Masaaki Mitani, Hideyuki Takahashi, Hitoshi Ohta
    • Journal Title

      Journal of Inorganic Biochemistry

      Volume: 162 Pages: 190-193

    • DOI

      10.1016/j.jinorgbio.2016.04.016

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of High-Pressure ESR System Using Micro-coil2015

    • Author(s)
      K. Kawasaki, T. Sakurai, E. Ohmichi, S. Okubo, H. Ohta, K. Matsubayashi, Y. Uwatoko
    • Journal Title

      Appl. Magn. Reson.

      Volume: 46 Issue: 9 Pages: 987-992

    • DOI

      10.1007/s00723-015-0650-y

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mechanical detection of electron spin resonance beyond 1 THz2015

    • Author(s)
      H. Takahashi, E. Ohmichi, H. Ohta
    • Journal Title

      Appl. Phys. Lett.

      Volume: 107 Issue: 18

    • DOI

      10.1063/1.4935204

    • NAID

      120005842525

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Multi-Extreme THz ESR: Recent Developments and Future2016

    • Author(s)
      太田 仁
    • Organizer
      Modern Development of Magnetic Resonance
    • Place of Presentation
      Academy of Sciences of the Republic of Tatarstan, Kazan, Russia
    • Year and Date
      2016-10-31
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multi-extreme THz ESR: New developments and applications2016

    • Author(s)
      太田 仁
    • Organizer
      Asia-Pacific EPR-ESR Symposium 2016
    • Place of Presentation
      Mayak Hotel, Irkutsk, Russia
    • Year and Date
      2016-08-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Exotic Quantum Spin Systems Studied by Multi-Extreme THz EMR2016

    • Author(s)
      太田 仁
    • Organizer
      The 4th Forum EMR-PL
    • Place of Presentation
      Morasko AMU campus, Poznan, Poland
    • Year and Date
      2016-06-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ピエゾ抵抗型マイクロカンチレバーを用いた力検出高周波ESR測定法の開発2016

    • Author(s)
      岡本翔、高橋英幸、大道英二、太田仁
    • Organizer
      日本物理学会第71回年次大会
    • Place of Presentation
      東北学院大学(宮城県・仙台市)
    • Year and Date
      2016-03-21
    • Related Report
      2015 Research-status Report
  • [Presentation] 多重極限テラヘルツESRの開発とその量子スピン系研究への応用2015

    • Author(s)
      太田仁、大久保晋、大道英二、櫻井敬博、高橋英幸
    • Organizer
      第54回電子スピンサイエンス学会年会(SEST2015)
    • Place of Presentation
      朱鷺メッセ(新潟県・新潟市)
    • Year and Date
      2015-11-03
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 磁化検出高周波ESR測定法のタンパク質への応用2015

    • Author(s)
      岡本翔、高橋英幸、大道英二、太田仁
    • Organizer
      第54回電子スピンサイエンス学会年会(SEST2015)
    • Place of Presentation
      朱鷺メッセ(新潟県・新潟市)
    • Year and Date
      2015-11-03
    • Related Report
      2015 Research-status Report
  • [Presentation] 多重極限THz ESRの開発とその応用2015

    • Author(s)
      太田仁、櫻井敬博、大久保晋、大道英二、高橋英幸
    • Organizer
      第25回日本赤外線学会研究発表会
    • Place of Presentation
      中部大学(愛知県・春日井市)
    • Year and Date
      2015-10-23
    • Related Report
      2015 Research-status Report
  • [Presentation] カンチレバーを用いたヘムたんぱく質の高周波ESR測定装置の改良2015

    • Author(s)
      岡本翔、高橋英幸、大道英二、太田仁
    • Organizer
      日本物理学会2015年秋季大会
    • Place of Presentation
      関西大学(大阪府・吹田市)
    • Year and Date
      2015-09-16
    • Related Report
      2015 Research-status Report
  • [Presentation] Multi-extremeTHz ESR: Its Developments and Applications2015

    • Author(s)
      H. Ohta, S. Okubo, E. Ohmichi, T. Sakurai
    • Organizer
      The Second International Symposium on Frontiers in THz Technology (FTT2015)
    • Place of Presentation
      アクトシティ浜松(静岡県・浜松市)
    • Year and Date
      2015-08-31
    • Related Report
      2015 Research-status Report
  • [Presentation] Development for ultrasensitive terahertz ESR of metal protein using a microcantilever2015

    • Author(s)
      T. Okamoto, H. Takahashi, E. Ohmichi, H. Ohta
    • Organizer
      The Second International Symposium on Frontiers in THz Technology (FTT2015)
    • Place of Presentation
      アクトシティ浜松(静岡県・浜松市)
    • Year and Date
      2015-08-30
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Multi-Extreme THz ESR: Developments and Its Application to Multiferroic Substance YCrO32015

    • Author(s)
      H. Ohta, S. Okubo, E. Ohmichi, T. Sakurai, H. Takahashi, S. Ikeda, S. Hara, H. Sakurai
    • Organizer
      International Society of Magnetic Resonance (ISMAR2015)
    • Place of Presentation
      Shanghai (China)
    • Year and Date
      2015-08-17
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of cantilever-detected ESR technique for small-volume heme protein analysis2015

    • Author(s)
      T. Okamoto, E. Ohmichi, H. Ohta
    • Organizer
      The 3rd Awaji International Workshop on Electron Spin Science & Technology (AWEST 2015)
    • Place of Presentation
      淡路夢舞台(兵庫県・淡路市)
    • Year and Date
      2015-06-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Multi-extreme THz ESR systems and Its Application to Quantum Spin Substance2015

    • Author(s)
      H. Ohta, S. Okubo, E. Ohmichi, T. Sakurai. H. Takahashi
    • Organizer
      The 3rd Awaji International Workshop on Electron Spin Science & Technology (AWEST 2015)
    • Place of Presentation
      淡路夢舞台(兵庫県・淡路市)
    • Year and Date
      2015-06-14
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] カンチレバーを用いた高周波ESR測定法の金属タンパク質への応用2015

    • Author(s)
      岡本翔
    • Organizer
      日本物理学会 2015年春季大会
    • Place of Presentation
      早稲田大学
    • Year and Date
      2015-03-20 – 2015-03-24
    • Related Report
      2014 Research-status Report
  • [Presentation] カンチレバーを用いた高周波ESR測定法の生体試料への応用2014

    • Author(s)
      岡本翔
    • Organizer
      若手フロンティア研究会2014
    • Place of Presentation
      神戸大学
    • Year and Date
      2014-12-25
    • Related Report
      2014 Research-status Report
  • [Presentation] カンチレバーを用いた高分解能生体ESR測定に向けた試み2014

    • Author(s)
      岡本翔
    • Organizer
      日本赤外線学会 第24回研究発表会
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2014-11-27 – 2014-11-28
    • Related Report
      2014 Research-status Report
  • [Presentation] Application of cantilever-detected ESR to biological systems2014

    • Author(s)
      T. Okamoto
    • Organizer
      APES-IES-SEST 2014
    • Place of Presentation
      Nara, Japan
    • Year and Date
      2014-11-12 – 2014-11-16
    • Related Report
      2014 Research-status Report
  • [Presentation] Multi-Extreme THz ESR Systems in Kobe2014

    • Author(s)
      H. Ohta
    • Organizer
      International Symposium “Catalytic Systems for Chemical Energy Conversion”
    • Place of Presentation
      Muelheim an der Ruhr, Germany
    • Year and Date
      2014-07-23 – 2014-07-25
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Developments and applications of multi-extreme THz ESR2014

    • Author(s)
      H. Ohta
    • Organizer
      Magnetic Resonance: fundamental research and pioneering applications (MR-70)
    • Place of Presentation
      Kazan, Russia
    • Year and Date
      2014-06-23 – 2014-06-27
    • Related Report
      2014 Research-status Report
    • Invited

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Published: 2014-04-04   Modified: 2020-05-15  

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