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Analysis of the mechanism of biological soliton by gene anaylysis

Research Project

Project/Area Number 26610129
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biological physics/Chemical physics/Soft matter physics
Research InstitutionUniversity of Tsukuba

Principal Investigator

KUWAYAMA Hidekazu  筑波大学, 生命環境系, 准教授 (40397659)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsソリトン / 孤立波(ソリトン) / 細胞集団運動
Outline of Final Research Achievements

By the next generation DNA sequencer, we determined all the genome sequences of two soliton strains, KI-5 and KI-10, and their parental strain. By analysis of SNP, 75 genes of KI-5 and 75 genes of KI-10 were found to be changed. Furthermore, by deep-sequencing, 105 genes of KI-5 and 19 genes of KI-10 were found to increase during soliton.
Next we tried to look up the responsible genes. Among those mutated genes, one gene was found to be related to soliton phenomenon by molecular biology technique.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2015 2014

All Presentation (6 results)

  • [Presentation] 細胞性粘菌の形態形成における走化性運動の役割についての解析2015

    • Author(s)
      潘愷、桑山秀一
    • Organizer
      日本分子生物学会年会、日本生化学会年会大会、合同大会
    • Place of Presentation
      神戸ポートアイランド、兵庫県神戸市
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] ソリトン波様細胞集団運動における接着分子の役割2015

    • Author(s)
      桑山秀一
    • Organizer
      日本分子生物学会年会、日本生化学会年会大会、合同大会
    • Place of Presentation
      神戸ポートアイランド、兵庫県神戸市
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞性粘菌の形態形成における走化性運動の役割についての解析2015

    • Author(s)
      潘愷、桑山秀一
    • Organizer
      日本細胞性粘菌学会例会
    • Place of Presentation
      弘前大学、青森弘前市
    • Year and Date
      2015-10-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] 走化性不能株を用いた細胞性粘菌の形態形成における細胞接着性の重要性2015

    • Author(s)
      潘愷、桑山秀一
    • Organizer
      日本細胞生物学会
    • Place of Presentation
      タワーホール船堀、東京都江戸川区
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] ソリトン波様細胞集団運動における接着分子の役割2014

    • Author(s)
      桑山 秀一
    • Organizer
      第4回日本細胞性粘菌学会例会
    • Place of Presentation
      仙台市、東北大学
    • Year and Date
      2014-10-11 – 2014-10-12
    • Related Report
      2014 Research-status Report
  • [Presentation] ソリトン波様細胞集団運動における接着分子の役割2014

    • Author(s)
      桑山 秀一
    • Organizer
      第66回日本細胞生物学会大会
    • Place of Presentation
      奈良県奈良市、奈良県新公会堂
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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