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Development of transmission super-resolution microscope and visualization of early steps of photosynthesis

Research Project

Project/Area Number 26610133
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biological physics/Chemical physics/Soft matter physics
Research InstitutionOsaka City University

Principal Investigator

SUGISAKI Mitsuru  大阪市立大学, 大学院理学研究科, 准教授 (20360042)

Co-Investigator(Renkei-kenkyūsha) HASHIMOTO HIDEKI  関西学院大学, 理工学部, 教授 (50222211)
FUJII RITSUKO  大阪市立大学, 複合先端研究機構, 准教授 (80351740)
KOSUMI DAISUKE  熊本大学, パルスパワー科学研究所, 准教授 (70613149)
Research Collaborator FUNAKOSHI RYOUHEI  大阪市立大学, 大学院理学研究科, 大学院生
EMURA HIDETOSHI  大阪市立大学, 大学院理学研究科, 大学院生
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords顕微鏡法 / 光合成 / 超解像度顕微鏡 / 光物性 / ナノテクノロジー / 量子光学 / エネルギー問題
Outline of Final Research Achievements

A new methodology to observe super-resolution microscopic images using unstained specimens has been investigated. Here we focus our attention on the early steps of photosynthesis; the super-resolution microscope developed in this project was adjusted thus that the highest sensitivity can be attained in the near-infrared region. The optical properties of typical photosynthetic pigments, especially ultrafast phenomena and optical nonlinearity, have been investigated since these phenomena should be prominent in the optical measurements using high-resolution microscopes. It was found that very strong anti-Stokes fluorescence peaks appear from chlorophyll molecules. The ultrafast optical response of carotenoid molecules has also been investigated by comparing the experimental results with theoretical calculations.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (12 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (3 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Invited: 1 results)

  • [Int'l Joint Research] University of Toronto(Canada)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Toronto(Canada)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] University of Glasgow(英国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Photoprotection Mechanism of Light-Harvesting Antenna Complex from Purple Bacteria2016

    • Author(s)
      D. Kosumi, T. Horibe, M. Sugisaki, R.J. Cogdell, H. Hashimoto
    • Journal Title

      J. Phys. Chem. B

      Volume: 120 Issue: 5 Pages: 951-956

    • DOI

      10.1021/acs.jpcb.6b00121

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Ultrafast coherent spectroscopic investigation on photosynthetic pigment chlorophyll a utilizing 20 fs pulses2015

    • Author(s)
      D. Kosumi, T. Nishiguchi, M. Sugisaki, H. Hashimoto
    • Journal Title

      J. Photochem. Photobiol. A

      Volume: 313 Pages: 72-78

    • DOI

      10.1016/j.jphotochem.2015.06.025

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] How do surrounding environments influence the electronic and vibrational properties of spheroidene?2015

    • Author(s)
      N. Tonouchi
    • Journal Title

      Photosynth. Res.

      Volume: 124 Issue: 1 Pages: 77-86

    • DOI

      10.1007/s11120-015-0095-z

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] スペクトル分解をしたβ-カロテンの四光波混合信号 II2017

    • Author(s)
      杉﨑 満
    • Organizer
      日本物理学会第72回年次大会
    • Place of Presentation
      大阪大学
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] スペクトル分解をしたβ-カロテンの四光波混合信号2016

    • Author(s)
      杉﨑 満
    • Organizer
      日本物理学会2016年秋季大会
    • Place of Presentation
      金沢大学
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] スフェロイデンにおける振動緩和の溶媒効果2015

    • Author(s)
      船越良平
    • Organizer
      日本物理学会 第70回年次大会
    • Place of Presentation
      早稲田大学 早稲田キャンパス (東京都新宿区)
    • Year and Date
      2015-03-21 – 2015-03-24
    • Related Report
      2014 Research-status Report
  • [Presentation] 周辺環境は電子及び振動ダイナミクスにどのように影響を及ぼすのか?2014

    • Author(s)
      殿内 規之
    • Organizer
      第28回カロテノイド研究談話会
    • Place of Presentation
      石川県文教会館 (石川県金沢市)
    • Year and Date
      2014-09-04 – 2014-09-05
    • Related Report
      2014 Research-status Report
  • [Presentation] How do surrounding environments influence the electronic and vibrational properties of spheroidene?2014

    • Author(s)
      N. Tonouchi
    • Organizer
      17th International Carotenoid Symposium
    • Place of Presentation
      Utah, USA
    • Year and Date
      2014-06-29 – 2014-07-04
    • Related Report
      2014 Research-status Report
  • [Presentation] Ultrafast excited state dynamics of marine carotenoid fucoxanthin and its homologues2014

    • Author(s)
      H. Hashimoto
    • Organizer
      17th International Carotenoid Symposium
    • Place of Presentation
      Utah, USA
    • Year and Date
      2014-06-26 – 2014-07-04
    • Related Report
      2014 Research-status Report
    • Invited

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Published: 2014-04-04   Modified: 2022-02-03  

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