Development of transmission super-resolution microscope and visualization of early steps of photosynthesis
| Project/Area Number |
26610133
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biological physics/Chemical physics/Soft matter physics
|
|---|
| Research Institution | Osaka City University |
|---|
Principal Investigator |
SUGISAKI Mitsuru 大阪市立大学, 大学院理学研究科, 准教授 (20360042)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
HASHIMOTO HIDEKI 関西学院大学, 理工学部, 教授 (50222211)
FUJII RITSUKO 大阪市立大学, 複合先端研究機構, 准教授 (80351740)
KOSUMI DAISUKE 熊本大学, パルスパワー科学研究所, 准教授 (70613149)
|
|---|
| Research Collaborator |
FUNAKOSHI RYOUHEI 大阪市立大学, 大学院理学研究科, 大学院生
EMURA HIDETOSHI 大阪市立大学, 大学院理学研究科, 大学院生
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | 顕微鏡法 / 光合成 / 超解像度顕微鏡 / 光物性 / ナノテクノロジー / 量子光学 / エネルギー問題 |
|---|
| Outline of Final Research Achievements |
A new methodology to observe super-resolution microscopic images using unstained specimens has been investigated. Here we focus our attention on the early steps of photosynthesis; the super-resolution microscope developed in this project was adjusted thus that the highest sensitivity can be attained in the near-infrared region. The optical properties of typical photosynthetic pigments, especially ultrafast phenomena and optical nonlinearity, have been investigated since these phenomena should be prominent in the optical measurements using high-resolution microscopes. It was found that very strong anti-Stokes fluorescence peaks appear from chlorophyll molecules. The ultrafast optical response of carotenoid molecules has also been investigated by comparing the experimental results with theoretical calculations.
|
Report
(4 results)
Research Products
(12 results)
