• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study of protein structure using non selective multifrequency pulsed EPR

Research Project

Project/Area Number 26620003
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Physical chemistry
Research InstitutionNagoya University

Principal Investigator

Mino Hiroyuki  名古屋大学, 理学研究科, 准教授 (70300902)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsEPR / PELDOR / 多周波共鳴 / 光化学系Ⅱ / ELDOR
Outline of Final Research Achievements

Pulsed EPR is the essential tool for investigation of unpaired electron. Generally, the EPR linewidth is very broad, and it is difficult to measure spin packets in pulsed EPR. By mixing multi frequency microwaves, detectable frequency range in EPR lines was 3-5 times increased. The technique was applied to the centers in proteins. It gave high quality signal under the low radical contentraion.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (22 results)

All 2017 2016 2015

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results,  Acknowledgement Compliant: 4 results) Presentation (15 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] Hyperfine Sublevel Correlation Spectroscopy Studies of Iron-Sulfur Cluster in Rieske Protein from Green Sulfur Bacterium Chlorobaculum tepidum2017

    • Author(s)
      Hiroki Nagashima, Hiraku Kishimoto, Risa Mutton, Naotaka Terashima, Hirozo Oh-Oka, Genji Kurisu, Hiroyuki Mino
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 121 Issue: 12 Pages: 2543-2553

    • DOI

      10.1021/acs.jpcb.6b12968

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Regulated Electron Tunneling of Photoinduced Primary Charge-Separated State in the Photosystem II Reaction Center2017

    • Author(s)
      M. Hasegawa, H. Nagashima, R. Minobe, T. Tachikawa, *H. Mino and *Y. Kobori
    • Journal Title

      J. Phys. Chem. Lett.

      Volume: 8 Issue: 6 Pages: 11791184-11791184

    • DOI

      10.1021/acs.jpclett.7b00044

    • NAID

      120006305568

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Location of Methanol on the S2 State Mn Cluster in Photosystem II Studied by Proton Matrix Electron Nuclear Double Resonance2017

    • Author(s)
      Hiroki Nagashima, Hiroyuki Mino
    • Journal Title

      The journal of physical chemistry letters

      Volume: 8 Issue: 3 Pages: 621-625

    • DOI

      10.1021/acs.jpclett.7b00110

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Electronic Structure of Manganese Cluster in Photosystem II Investigated by ESR2017

    • Author(s)
      Mizue Asada, Hiroki Nagashima, Hiroyuki Mino
    • Journal Title

      Seibutsu Butsuri

      Volume: 57 Issue: 1 Pages: 011-014

    • DOI

      10.2142/biophys.57.011

    • NAID

      130005296470

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proton matrix ENDOR studies on Ca2+-depleted and Sr2+-substituted Mn cluster in photosystem II2015

    • Author(s)
      Nagashima,H., Nakajima, Y., Shen, J-R. and Mino, H.
    • Journal Title

      J. Biol. Chem

      Volume: 290 Issue: 47 Pages: 28166-28174

    • DOI

      10.1074/jbc.m115.675496

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Location of the High-Affinity Mn2+ Site in Photosystem II Detected by PELDOR2015

    • Author(s)
      Asada, M and Mino, H.
    • Journal Title

      J. Phys. Chem. B.

      Volume: 119 Pages: 10139-10144

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Radical formation and proton pathway in Photozipper protein2017

    • Author(s)
      三野 広幸, 小関 康平,長嶋 宏樹,久冨 修
    • Organizer
      日本植物生理学会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 機能性タンパク質Photozipperの反応機構2016

    • Author(s)
      三野 広幸, 小関 康平,長嶋 宏樹,久冨 修
    • Organizer
      分子研研究会
    • Place of Presentation
      分子科学研究所
    • Year and Date
      2016-12-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] 機能性タンパク質Photozipperの反応過程の解析2016

    • Author(s)
      小関 康平,長嶋 宏樹,久冨 修, 三野 広幸
    • Organizer
      電子スピンサイエンス学会年会
    • Place of Presentation
      筑波大学
    • Year and Date
      2016-11-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] Chlorobaculum tepidum還元型 [2Fe-2S] Rieske clusterの磁気構造解析2016

    • Author(s)
      寺島尚貴、長嶋宏樹、岸本拓、武藤梨沙、大岡宏造、栗栖源嗣、三野広幸
    • Organizer
      電子スピンサイエンス学会年会
    • Place of Presentation
      筑波大学
    • Year and Date
      2016-11-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] 時間分解EPRによるヘリオバクテリア反応中心での初期ラジカル対生成2016

    • Author(s)
      佃野弘幸,武藤梨沙,栗栖源嗣、大岡宏造, 三野 広幸
    • Organizer
      電子スピンサイエンス学会年会
    • Place of Presentation
      筑波大学
    • Year and Date
      2016-11-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] Local structure of photosystem II revealed by pulsed ELDOR,Molecular Photoscience Research Center2016

    • Author(s)
      Hiroyuki Mino
    • Organizer
      MR-THz2016),
    • Place of Presentation
      Kobe-university
    • Year and Date
      2016-11-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Ca2+除去したマンガンクラスターの高酸化状態スピン構造2015

    • Author(s)
      酒井貴弘、長嶋宏樹、松下仁美、三野広幸
    • Organizer
      第54回電子スピンサイエンス学会年会
    • Place of Presentation
      新潟コンベンションセンター
    • Year and Date
      2015-11-02
    • Related Report
      2015 Research-status Report
  • [Presentation] 光化学系II表在性サブユニットPsbP結合位置の解析2015

    • Author(s)
      浅田瑞枝、西村大志、佐藤文彦、伊福健太郎、三野広幸
    • Organizer
      電子スピンサイエンス学会年会
    • Place of Presentation
      新潟コンベンションセンター
    • Year and Date
      2015-11-02
    • Related Report
      2015 Research-status Report
  • [Presentation] プロトンENDORによるアンモニア分子のMnクラスターへの結合サイトの同定2015

    • Author(s)
      長嶋宏樹, 三野広幸
    • Organizer
      第54回電子スピンサイエンス学会年会,
    • Place of Presentation
      新潟コンベンションセンター
    • Year and Date
      2015-11-02
    • Related Report
      2015 Research-status Report
  • [Presentation] ENDOR studies on biochemical modification on calcium site of the Mn cluster in photosystem II2015

    • Author(s)
      長嶋宏樹, 中島芳樹, 沈建仁, 三野広幸
    • Organizer
      第53回生物物理学会年会
    • Place of Presentation
      金沢大学
    • Year and Date
      2015-09-13
    • Related Report
      2015 Research-status Report
  • [Presentation] PELDOR study on the high-affinity Mn(II) site of photoactivation of photosystem II2015

    • Author(s)
      浅田瑞枝、三野広幸
    • Organizer
      日本生物物理学会年会
    • Place of Presentation
      金沢大学
    • Year and Date
      2015-09-13
    • Related Report
      2015 Research-status Report
  • [Presentation] パルスELDOR法を用いた光化学系II Mnクラスターの光活性化におけるMn2+親和サイトの位置決定2015

    • Author(s)
      浅田瑞枝,三野広幸
    • Organizer
      第19回ESRフォーラム
    • Place of Presentation
      東京工業大学
    • Year and Date
      2015-07-24
    • Related Report
      2015 Research-status Report
  • [Presentation] ENDOR Studies on the Hydrogen-Bonding Network Surrounding Mn Cluster"2015

    • Author(s)
      Hiroki Nagashima, and Hiroyuki Mino
    • Organizer
      理研シンポジウム: 生体金属科学
    • Place of Presentation
      理化学研究所(和光)
    • Year and Date
      2015-06-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Local structure and function of oxygen-evolving complex in photosystem II investigated by Electron Spin Resonance2015

    • Author(s)
      Hiroyuki Mino, Wataru Koinuma, Hiroki Nagashima, Takahiro Sakai, Mizue Asada
    • Organizer
      The 3nd Awaji International workshop on Electron Spin Science and Technology
    • Place of Presentation
      Awaji Yumebutai. Hyogo
    • Year and Date
      2015-06-14
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Position of the Mn(II) binding site at the initial step of photoactivation of photosystem II studied by PELDOR2015

    • Author(s)
      Mizue Asada, Hiroyuki Mino
    • Organizer
      The 3nd Awaji International workshop on Electron Spin Science and Technology
    • Place of Presentation
      Awaji Yumebutai. Hyogo
    • Year and Date
      2015-06-14
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] 光合成のエネルギー変換と物質変換2015

    • Author(s)
      三野 広幸
    • Total Pages
      2
    • Publisher
      化学同人
    • Related Report
      2015 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi