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Rapid detection of food poisoning bacteria by using light-antenna

Research Project

Project/Area Number 26620072
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Functional solid state chemistry
Research InstitutionOsaka Prefecture University

Principal Investigator

SHIIGI Hiroshi  大阪府立大学, 工学(系)研究科(研究院), 准教授 (70335769)

Co-Investigator(Renkei-kenkyūsha) NAGAOKA TSUTOMU  大阪府立大学, 工学研究科, 教授 (00172510)
NISHINO TOMOAKI  東京工業大学, 大学院理学研究科, 准教授 (80372415)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords光アンテナ / 単一細菌 / ナノ構造体 / バイオ分析 / O157
Outline of Final Research Achievements

We have found that a high density structure of metal nanoparticles acts like an
excellent antenna owing to its optical properties, which permit sensitive detection of bacteria and analysis of a single bacterial cell. By using antibodies, these antennas can be engineered to recognize only specific bacterial species. This system provides a new technique that will allow a more sensitive detection of specific bacteria.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (40 results)

All 2016 2015 2014 Other

All Journal Article (9 results) (of which Peer Reviewed: 8 results,  Open Access: 4 results,  Acknowledgement Compliant: 4 results) Presentation (26 results) (of which Int'l Joint Research: 5 results,  Invited: 2 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Optical Characterization of Gold Nanoparticle Layers Formed on Plastic Microbeads2016

    • Author(s)
      H. Shiigi, T. Fujita, X. Shan, M. Terabe, A. Mihashi, Y. Yamamoto, T. Nagaoka
    • Journal Title

      Analytical Sciences

      Volume: 32 Issue: 3 Pages: 281-285

    • DOI

      10.2116/analsci.32.281

    • NAID

      130005131625

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Light-scattering Characteristics of Metal Nanoparticles on a Single Bacterial Cell2016

    • Author(s)
      T. Kinoshita, K. Kiso, D. Q. Le, H. Shiigi, T. Nagaoka
    • Journal Title

      Analytical Sciences

      Volume: 32 Issue: 3 Pages: 301-305

    • DOI

      10.2116/analsci.32.301

    • NAID

      130005131634

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] One-by-one single-molecule detection of mutated nucleobases by monitoring tunneling current using a DNA tip2015

    • Author(s)
      P. T. Bui, T. Nishino, H. Shiigi, T. Nagaoka
    • Journal Title

      Chem. Commun.,

      Volume: 51 Issue: 9 Pages: 1666-1669

    • DOI

      10.1039/c4cc08227c

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nanoantennas as biomarkers for bacterial detection2015

    • Author(s)
      H. Shiigi, T. Kinoshita, M. Fukuda, D. Q. Le, T. Nishino, and T. Nagaoka
    • Journal Title

      Anal. Chem.

      Volume: 87 Issue: 7 Pages: 4042-4046

    • DOI

      10.1021/acs.analchem.5b00415

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescence Enhancement of Nanoraspberry Hot-spot Source Composed of Gold Nanoparticles and Aniline Oligomers2015

    • Author(s)
      T. Kinoshita, N. Q. Dung, T. Nishino, H. Nakao, H. Shiigi, T. Nagaoka
    • Journal Title

      Analytical Sciences

      Volume: 31 Issue: 6 Pages: 487-493

    • DOI

      10.2116/analsci.31.487

    • NAID

      130005075718

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Optical Evaluation of the Surface Coverage of Silver Nanoparticle-coated Plastic Microbeads2015

    • Author(s)
      H. Shiigi, S. Kimura, T. Fujita, T. Nagaoka
    • Journal Title

      Analytical Sciences

      Volume: 31 Issue: 7 Pages: 577-579

    • DOI

      10.2116/analsci.31.577

    • NAID

      130005087181

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 光アンテナの形成による病原性細菌の迅速検出2015

    • Author(s)
      木下隆将, 椎木 弘, 長岡 勉
    • Journal Title

      化学工学

      Volume: 66 Pages: 812-816

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Construction of nanoantennas on the bacterial outer membrane2014

    • Author(s)
      H. Shiigi, M. Fukuda, T. Tono, K. Takada, T. Okada, L. Q. Dung, Y. Hatsuoka, T. Kinoshita, M. Takai, S. Tokonami, H. Nakao, T. Nishino, Y. Yamamoto. T. Nagaoka
    • Journal Title

      Chemical Communications

      Volume: 50 Issue: 47 Pages: 6252-6255

    • DOI

      10.1039/c4cc01204f

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fluorescent Carbon Nanowire Made by Pyrolysis of DNA Nanofiber and Plasmon-Assisted Emission Enhancement of Its Fluorescence2014

    • Author(s)
      H. Nakao, S. Tokonami, Y. Yamamoto, H. Shiigi and Y. Takeda
    • Journal Title

      Chem. Commun.

      Volume: 50 Issue: 80 Pages: 11887-11890

    • DOI

      10.1039/c4cc04647a

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Optical nanoantenna for bacterial detection2015

    • Author(s)
      M. Fukuda, T. Kinoshita, H. Shiigi, T. Nagaoka
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      ハワイコンベンションセンター,ホノルル,米国
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 機能的ナノ空間の創成と分子認識への応用2015

    • Author(s)
      椎木弘
    • Organizer
      第61回ポーラログラフィおよび電気分析化学討論会
    • Place of Presentation
      イーグレ姫路,姫路,兵庫
    • Year and Date
      2015-11-25
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Molecular Sensing on Microbead2015

    • Author(s)
      Y. Takai, M. Terabe, T. Nishino, S. Tokonami, H. Shiigi, T. Nagaoka
    • Organizer
      Asian Conference on Chemical Sensor
    • Place of Presentation
      シャングリラリゾート,ペナン,マレーシア
    • Year and Date
      2015-11-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nanoantenna for Bacterial Detection2015

    • Author(s)
      T. Kinoshita, M. Fukuda, D. Nguyen, K. Ishiki, T. Nishino, H. Shiigi, T. Nagaoka
    • Organizer
      Asian Conference on Chemical Sensor
    • Place of Presentation
      シャングリラリゾート,ペナン,マレーシア
    • Year and Date
      2015-11-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Optical Detection of Pathogenic Bacteria Using Nanocomposite as a Biomarker2015

    • Author(s)
      T. Kinoshita, M. Fukuda, H. Shiigi, T. Nagaoka
    • Organizer
      7th East Symposium on Functional Dyes and Advanced Materials
    • Place of Presentation
      大阪府立大学,堺,大阪
    • Year and Date
      2015-09-02
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fluorescence Enhancement of Nanoraspberry Composed of Gold Nanoparticles and Aniline Oligomer2015

    • Author(s)
      N. D. Quang, T. Kinoshita, H. Shiigi, T. Nagaoka
    • Organizer
      7th East Symposium on Functional Dyes and Advanced Materials
    • Place of Presentation
      大阪府立大学,堺,大阪
    • Year and Date
      2015-09-02
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 導電/絶縁性プラスチックを用いた微細構造の形成2015

    • Author(s)
      寺部政大,高井善朗,西野智昭,椎木 弘,長岡 勉
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      日本大学(千葉県船橋市)
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 金属ナノ粒子によるバクテリアへの光アンテナ形成2015

    • Author(s)
      石木健吾,西野智昭,椎木 弘,長岡 勉
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      日本大学(千葉県船橋市)
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 高密度金ナノ粒子集合体を標識としたバクテリアの光学的検出2015

    • Author(s)
      木下隆将,福田真帆,西野智昭,椎木 弘,長岡 勉
    • Organizer
      第62回応用物理学会春季学術講演会
    • Place of Presentation
      東海大学(神奈川県平塚市)
    • Year and Date
      2015-03-15 – 2015-03-17
    • Related Report
      2014 Research-status Report
  • [Presentation] 金属ナノ粒子を光アンテナとしたバクテリアの検出2015

    • Author(s)
      木下隆将,福田真帆,西野智昭,椎木 弘,長岡 勉
    • Organizer
      Bio Medical Forum 2015
    • Place of Presentation
      大阪府立大学(大阪府堺市)
    • Year and Date
      2015-01-23
    • Related Report
      2014 Research-status Report
  • [Presentation] 金ナノ粒子固定ビーズの電気的特性評価2014

    • Author(s)
      寺部政大, 山本陽二郎,椎木 弘,長岡 勉
    • Organizer
      第3回関西電気化学研究会
    • Place of Presentation
      大阪大学(大阪府豊中市)
    • Year and Date
      2014-12-13
    • Related Report
      2014 Research-status Report
  • [Presentation] 異化金属還元細菌を用いた金属イオンの還元機構2014

    • Author(s)
      石木健吾,西野智昭,椎木 弘,長岡 勉
    • Organizer
      第3回関西電気化学研究会
    • Place of Presentation
      大阪大学(大阪府豊中市)
    • Year and Date
      2014-12-13
    • Related Report
      2014 Research-status Report
  • [Presentation] 金ナノ粒子被覆磁性マイクロビーズを用いた電気化学的バイオ分析法の開発2014

    • Author(s)
      木下隆将,山本陽二郎,椎木 弘,長岡 勉
    • Organizer
      第3回関西電気化学研究会
    • Place of Presentation
      大阪大学(大阪府豊中市)
    • Year and Date
      2014-12-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Electron Microscopic Tracking Growth Process of Tadpole-Shaped Hybrid Composed of Au NPs and Polyaniline2014

    • Author(s)
      T. Kinoshita, M. Fukuda, T. Nishino, H. Shiigi, T. Nagaoka
    • Organizer
      ICFIA19
    • Place of Presentation
      アクロス福岡(福岡県福岡市)
    • Year and Date
      2014-11-30 – 2014-12-05
    • Related Report
      2014 Research-status Report
  • [Presentation] 隙間の化学2014

    • Author(s)
      椎木 弘, 西野智昭, 床波志保, 長岡 勉
    • Organizer
      第60回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      京都工芸繊維大学(京都府京都市)
    • Year and Date
      2014-11-15 – 2014-11-16
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 金属ナノアンテナを用いたバクテリアの迅速検出法の開発2014

    • Author(s)
      木下隆将, 福田真帆, 西野智昭, 椎木 弘, 長岡 勉
    • Organizer
      第60回ポーラログラフィーおよび電気分析化学討論会
    • Place of Presentation
      京都工芸繊維大学(京都府京都市)
    • Year and Date
      2014-11-15 – 2014-11-16
    • Related Report
      2014 Research-status Report
  • [Presentation] 金ナノ粒子集合体による蛍光増強機構2014

    • Author(s)
      木下隆将, 西野智昭,椎木 弘,長岡 勉
    • Organizer
      第75回応用物理学会秋季学術講演会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2014-09-17 – 2014-09-20
    • Related Report
      2014 Research-status Report
  • [Presentation] 金ナノ粒子をアンテナとしたバクテリア検出2014

    • Author(s)
      福田真帆, 初岡 優, L. D. Quynh, 西野智昭, 椎木 弘, 長岡 勉
    • Organizer
      第75回応用物理学会秋季学術講演会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2014-09-17 – 2014-09-20
    • Related Report
      2014 Research-status Report
  • [Presentation] 金被覆マイクロビーズの表面プラズモン共鳴を利用したセンシング2014

    • Author(s)
      高井善朗, 寺部政大, 阪口仁美, 西野智昭, 山本陽二郎, 椎木 弘
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学(広島県東広島市)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Research-status Report
  • [Presentation] Formation of Nanoantenna on a Bacterium2014

    • Author(s)
      Y. Hastuoka, M. Fukuda, T. Kinoshita, S. Tokonami, H. Nakao, T. Nishino, H. Shiigi, T. Nagaoka
    • Organizer
      XIII European Vacuum Conference
    • Place of Presentation
      Aveiro (Portugal)
    • Year and Date
      2014-09-08 – 2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] Electron Microscopic Tracking Growth Process of Tadpole-Shaped Nanoparticles Aggregate2014

    • Author(s)
      T. Kinoshita, Y. Hastuoka, H. Nakao, T. Nishino, Y. Yamamoto, H. Shiigi, T. Nagaoka
    • Organizer
      XIII European Vacuum Conference
    • Place of Presentation
      Aveiro (Portugal)
    • Year and Date
      2014-09-08 – 2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] Electrical Properties of Two-Dimensional Gold Nanoparticle Array on Microbeads2014

    • Author(s)
      H. Shiigi, Y. Takai, T. Nishino, Y. Yamamoto, T. Nagaoka
    • Organizer
      XIII European Vacuum Conference
    • Place of Presentation
      Aveiro (Portugal)
    • Year and Date
      2014-09-08
    • Related Report
      2014 Research-status Report
  • [Presentation] Formation of Nanoantenna on a Bacterial Surface2014

    • Author(s)
      H. Shiigi, M. Fukuda, T. Kinoshita, Y. Hastuoka, T. Nishino, T. Nagaoka
    • Organizer
      ECOSS30
    • Place of Presentation
      Antalya (Turkey)
    • Year and Date
      2014-08-31 – 2014-09-05
    • Related Report
      2014 Research-status Report
  • [Presentation] 金ナノ粒子集合体による高感度光アンテナの作製2014

    • Author(s)
      木下隆将, 西野智昭, 山本陽二郎, 椎木 弘, 長岡 勉
    • Organizer
      新領域創成研究会2014 in ひろしま
    • Place of Presentation
      サテライトキャンパス広島(広島県広島市)
    • Year and Date
      2014-08-18 – 2014-08-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 金ナノ粒子によるバクテリア検出2014

    • Author(s)
      福田真帆, 初岡 優, L. D. Quynh, 西野智昭, 椎木 弘, 長岡 勉
    • Organizer
      新領域創成研究会2014 in ひろしま
    • Place of Presentation
      サテライトキャンパス広島(広島県広島市)
    • Year and Date
      2014-08-18 – 2014-08-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 金ナノ粒子被覆マイクロビーズを用いた分析法の開発2014

    • Author(s)
      木下隆将, 高井善朗, 山本陽二郎, 西野智昭, 椎木 弘, 長岡 勉
    • Organizer
      JPCA2014 アカデミックプラザ
    • Place of Presentation
      ビッグサイト東京(東京都江東区)
    • Year and Date
      2014-06-04 – 2014-06-06
    • Related Report
      2014 Research-status Report
  • [Book] 基礎分析化学2015

    • Author(s)
      椎木 弘, 長岡 勉
    • Total Pages
      198
    • Publisher
      朝倉書店
    • Related Report
      2015 Annual Research Report
  • [Book] 基礎分析化学(第9章 生物学的分析)2015

    • Author(s)
      椎木 弘, 長岡 勉
    • Total Pages
      208
    • Publisher
      朝倉書店
    • Related Report
      2014 Research-status Report
  • [Remarks] 大阪府立大学分子認識化学研究グループHP

    • URL

      http://www.chem.osakafu-u.ac.jp/ohka/ohka12/index.html

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
  • [Remarks] 大阪府立大学教員活動情報

    • URL

      http://kyoindb.osakafu-u.ac.jp/html/100599_ja.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 大阪府立大学教員活動情報

    • URL

      http://kyoindb.osakafu-u.ac.jp/html/100599_ja.html?k=%E6%A4%8E%E6%9C%A8%E5%BC%98

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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