Rapid detection of food poisoning bacteria by using light-antenna

• Project/Area Number: 26620072
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional solid state chemistry
• Research Institution: Osaka Prefecture University
• Principal Investigator: SHIIGI Hiroshi 大阪府立大学, 工学(系)研究科(研究院), 准教授 (70335769)
• Co-Investigator(Renkei-kenkyūsha): NAGAOKA TSUTOMU 大阪府立大学, 工学研究科, 教授 (00172510) ; NISHINO TOMOAKI 東京工業大学, 大学院理学研究科, 准教授 (80372415)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 光アンテナ / 単一細菌 / ナノ構造体 / バイオ分析 / O157

Outline of Final Research Achievements

We have found that a high density structure of metal nanoparticles acts like an
excellent antenna owing to its optical properties, which permit sensitive detection of bacteria and analysis of a single bacterial cell. By using antibodies, these antennas can be engineered to recognize only specific bacterial species. This system provides a new technique that will allow a more sensitive detection of specific bacteria.

Research Products

• [Journal Article] Optical Characterization of Gold Nanoparticle Layers Formed on Plastic Microbeads (2016)
  • Author(s): H. Shiigi, T. Fujita, X. Shan, M. Terabe, A. Mihashi, Y. Yamamoto, T. Nagaoka
  • Journal Title: Analytical Sciences Volume: 32 Issue: 3 Pages: 281-285
  • DOI: 10.2116/analsci.32.281
  • NAID: 130005131625
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Light-scattering Characteristics of Metal Nanoparticles on a Single Bacterial Cell (2016)
  • Author(s): T. Kinoshita, K. Kiso, D. Q. Le, H. Shiigi, T. Nagaoka
  • Journal Title: Analytical Sciences Volume: 32 Issue: 3 Pages: 301-305
  • DOI: 10.2116/analsci.32.301
  • NAID: 130005131634
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] One-by-one single-molecule detection of mutated nucleobases by monitoring tunneling current using a DNA tip (2015)
  • Author(s): P. T. Bui, T. Nishino, H. Shiigi, T. Nagaoka
  • Journal Title: Chem. Commun., Volume: 51 Issue: 9 Pages: 1666-1669
  • DOI: 10.1039/c4cc08227c
  • Peer Reviewed
• [Journal Article] Nanoantennas as biomarkers for bacterial detection (2015)
  • Author(s): H. Shiigi, T. Kinoshita, M. Fukuda, D. Q. Le, T. Nishino, and T. Nagaoka
  • Journal Title: Anal. Chem. Volume: 87 Issue: 7 Pages: 4042-4046
  • DOI: 10.1021/acs.analchem.5b00415
  • Peer Reviewed
• [Journal Article] Fluorescence Enhancement of Nanoraspberry Hot-spot Source Composed of Gold Nanoparticles and Aniline Oligomers (2015)
  • Author(s): T. Kinoshita, N. Q. Dung, T. Nishino, H. Nakao, H. Shiigi, T. Nagaoka
  • Journal Title: Analytical Sciences Volume: 31 Issue: 6 Pages: 487-493
  • DOI: 10.2116/analsci.31.487
  • NAID: 130005075718
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Optical Evaluation of the Surface Coverage of Silver Nanoparticle-coated Plastic Microbeads (2015)
  • Author(s): H. Shiigi, S. Kimura, T. Fujita, T. Nagaoka
  • Journal Title: Analytical Sciences Volume: 31 Issue: 7 Pages: 577-579
  • DOI: 10.2116/analsci.31.577
  • NAID: 130005087181
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 光アンテナの形成による病原性細菌の迅速検出 (2015)
  • Author(s): 木下隆将, 椎木 弘, 長岡 勉
  • Journal Title: 化学工学 Volume: 66 Pages: 812-816
• [Journal Article] Construction of nanoantennas on the bacterial outer membrane (2014)
  • Author(s): H. Shiigi, M. Fukuda, T. Tono, K. Takada, T. Okada, L. Q. Dung, Y. Hatsuoka, T. Kinoshita, M. Takai, S. Tokonami, H. Nakao, T. Nishino, Y. Yamamoto. T. Nagaoka
  • Journal Title: Chemical Communications Volume: 50 Issue: 47 Pages: 6252-6255
  • DOI: 10.1039/c4cc01204f
  • Peer Reviewed
• [Journal Article] Fluorescent Carbon Nanowire Made by Pyrolysis of DNA Nanofiber and Plasmon-Assisted Emission Enhancement of Its Fluorescence (2014)
  • Author(s): H. Nakao, S. Tokonami, Y. Yamamoto, H. Shiigi and Y. Takeda
  • Journal Title: Chem. Commun. Volume: 50 Issue: 80 Pages: 11887-11890
  • DOI: 10.1039/c4cc04647a
  • Peer Reviewed
• [Presentation] Optical nanoantenna for bacterial detection (2015)
  • Author(s): M. Fukuda, T. Kinoshita, H. Shiigi, T. Nagaoka
  • Organizer: Pacifichem 2015
  • Place of Presentation: ハワイコンベンションセンター，ホノルル，米国
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] 機能的ナノ空間の創成と分子認識への応用 (2015)
  • Author(s): 椎木弘
  • Organizer: 第61回ポーラログラフィおよび電気分析化学討論会
  • Place of Presentation: イーグレ姫路，姫路，兵庫
  • Year and Date: 2015-11-25
  • Invited
• [Presentation] Molecular Sensing on Microbead (2015)
  • Author(s): Y. Takai, M. Terabe, T. Nishino, S. Tokonami, H. Shiigi, T. Nagaoka
  • Organizer: Asian Conference on Chemical Sensor
  • Place of Presentation: シャングリラリゾート，ペナン，マレーシア
  • Year and Date: 2015-11-16
  • Int'l Joint Research
• [Presentation] Nanoantenna for Bacterial Detection (2015)
  • Author(s): T. Kinoshita, M. Fukuda, D. Nguyen, K. Ishiki, T. Nishino, H. Shiigi, T. Nagaoka
  • Organizer: Asian Conference on Chemical Sensor
  • Place of Presentation: シャングリラリゾート，ペナン，マレーシア
  • Year and Date: 2015-11-16
  • Int'l Joint Research
• [Presentation] Optical Detection of Pathogenic Bacteria Using Nanocomposite as a Biomarker (2015)
  • Author(s): T. Kinoshita, M. Fukuda, H. Shiigi, T. Nagaoka
  • Organizer: 7th East Symposium on Functional Dyes and Advanced Materials
  • Place of Presentation: 大阪府立大学，堺，大阪
  • Year and Date: 2015-09-02
  • Int'l Joint Research
• [Presentation] Fluorescence Enhancement of Nanoraspberry Composed of Gold Nanoparticles and Aniline Oligomer (2015)
  • Author(s): N. D. Quang, T. Kinoshita, H. Shiigi, T. Nagaoka
  • Organizer: 7th East Symposium on Functional Dyes and Advanced Materials
  • Place of Presentation: 大阪府立大学，堺，大阪
  • Year and Date: 2015-09-02
  • Int'l Joint Research
• [Presentation] 導電／絶縁性プラスチックを用いた微細構造の形成 (2015)
  • Author(s): 寺部政大，高井善朗，西野智昭，椎木 弘，長岡 勉
  • Organizer: 日本化学会第95春季年会
  • Place of Presentation: 日本大学（千葉県船橋市）
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] 金属ナノ粒子によるバクテリアへの光アンテナ形成 (2015)
  • Author(s): 石木健吾，西野智昭，椎木 弘，長岡 勉
  • Organizer: 日本化学会第95春季年会
  • Place of Presentation: 日本大学（千葉県船橋市）
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] 高密度金ナノ粒子集合体を標識としたバクテリアの光学的検出 (2015)
  • Author(s): 木下隆将，福田真帆，西野智昭，椎木 弘，長岡 勉
  • Organizer: 第62回応用物理学会春季学術講演会
  • Place of Presentation: 東海大学（神奈川県平塚市）
  • Year and Date: 2015-03-15 – 2015-03-17
• [Presentation] 金属ナノ粒子を光アンテナとしたバクテリアの検出 (2015)
  • Author(s): 木下隆将，福田真帆，西野智昭，椎木 弘，長岡 勉
  • Organizer: Bio Medical Forum 2015
  • Place of Presentation: 大阪府立大学（大阪府堺市）
  • Year and Date: 2015-01-23
• [Presentation] 金ナノ粒子固定ビーズの電気的特性評価 (2014)
  • Author(s): 寺部政大, 山本陽二郎，椎木 弘，長岡 勉
  • Organizer: 第3回関西電気化学研究会
  • Place of Presentation: 大阪大学（大阪府豊中市）
  • Year and Date: 2014-12-13
• [Presentation] 異化金属還元細菌を用いた金属イオンの還元機構 (2014)
  • Author(s): 石木健吾，西野智昭，椎木 弘，長岡 勉
  • Organizer: 第3回関西電気化学研究会
  • Place of Presentation: 大阪大学（大阪府豊中市）
  • Year and Date: 2014-12-13
• [Presentation] 金ナノ粒子被覆磁性マイクロビーズを用いた電気化学的バイオ分析法の開発 (2014)
  • Author(s): 木下隆将，山本陽二郎，椎木 弘，長岡 勉
  • Organizer: 第3回関西電気化学研究会
  • Place of Presentation: 大阪大学（大阪府豊中市）
  • Year and Date: 2014-12-13
• [Presentation] Electron Microscopic Tracking Growth Process of Tadpole-Shaped Hybrid Composed of Au NPs and Polyaniline (2014)
  • Author(s): T. Kinoshita, M. Fukuda, T. Nishino, H. Shiigi, T. Nagaoka
  • Organizer: ICFIA19
  • Place of Presentation: アクロス福岡（福岡県福岡市）
  • Year and Date: 2014-11-30 – 2014-12-05
• [Presentation] 隙間の化学 (2014)
  • Author(s): 椎木 弘, 西野智昭, 床波志保, 長岡 勉
  • Organizer: 第60回ポーラログラフィーおよび電気分析化学討論会
  • Place of Presentation: 京都工芸繊維大学（京都府京都市）
  • Year and Date: 2014-11-15 – 2014-11-16
  • Invited
• [Presentation] 金属ナノアンテナを用いたバクテリアの迅速検出法の開発 (2014)
  • Author(s): 木下隆将, 福田真帆, 西野智昭, 椎木 弘, 長岡 勉
  • Organizer: 第60回ポーラログラフィーおよび電気分析化学討論会
  • Place of Presentation: 京都工芸繊維大学（京都府京都市）
  • Year and Date: 2014-11-15 – 2014-11-16
• [Presentation] 金ナノ粒子集合体による蛍光増強機構 (2014)
  • Author(s): 木下隆将, 西野智昭，椎木 弘，長岡 勉
  • Organizer: 第75回応用物理学会秋季学術講演会
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2014-09-17 – 2014-09-20
• [Presentation] 金ナノ粒子をアンテナとしたバクテリア検出 (2014)
  • Author(s): 福田真帆, 初岡 優, L. D. Quynh, 西野智昭, 椎木 弘, 長岡 勉
  • Organizer: 第75回応用物理学会秋季学術講演会
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2014-09-17 – 2014-09-20
• [Presentation] 金被覆マイクロビーズの表面プラズモン共鳴を利用したセンシング (2014)
  • Author(s): 高井善朗, 寺部政大, 阪口仁美, 西野智昭, 山本陽二郎, 椎木 弘
  • Organizer: 日本分析化学会第63年会
  • Place of Presentation: 広島大学（広島県東広島市）
  • Year and Date: 2014-09-17 – 2014-09-19
• [Presentation] Formation of Nanoantenna on a Bacterium (2014)
  • Author(s): Y. Hastuoka, M. Fukuda, T. Kinoshita, S. Tokonami, H. Nakao, T. Nishino, H. Shiigi, T. Nagaoka
  • Organizer: XIII European Vacuum Conference
  • Place of Presentation: Aveiro (Portugal)
  • Year and Date: 2014-09-08 – 2014-09-12
• [Presentation] Electron Microscopic Tracking Growth Process of Tadpole-Shaped Nanoparticles Aggregate (2014)
  • Author(s): T. Kinoshita, Y. Hastuoka, H. Nakao, T. Nishino, Y. Yamamoto, H. Shiigi, T. Nagaoka
  • Organizer: XIII European Vacuum Conference
  • Place of Presentation: Aveiro (Portugal)
  • Year and Date: 2014-09-08 – 2014-09-12
• [Presentation] Electrical Properties of Two-Dimensional Gold Nanoparticle Array on Microbeads (2014)
  • Author(s): H. Shiigi, Y. Takai, T. Nishino, Y. Yamamoto, T. Nagaoka
  • Organizer: XIII European Vacuum Conference
  • Place of Presentation: Aveiro (Portugal)
  • Year and Date: 2014-09-08
• [Presentation] Formation of Nanoantenna on a Bacterial Surface (2014)
  • Author(s): H. Shiigi, M. Fukuda, T. Kinoshita, Y. Hastuoka, T. Nishino, T. Nagaoka
  • Organizer: ECOSS30
  • Place of Presentation: Antalya (Turkey)
  • Year and Date: 2014-08-31 – 2014-09-05
• [Presentation] 金ナノ粒子集合体による高感度光アンテナの作製 (2014)
  • Author(s): 木下隆将, 西野智昭, 山本陽二郎, 椎木 弘, 長岡 勉
  • Organizer: 新領域創成研究会2014 in ひろしま
  • Place of Presentation: サテライトキャンパス広島（広島県広島市）
  • Year and Date: 2014-08-18 – 2014-08-19
• [Presentation] 金ナノ粒子によるバクテリア検出 (2014)
  • Author(s): 福田真帆, 初岡 優, L. D. Quynh, 西野智昭, 椎木 弘, 長岡 勉
  • Organizer: 新領域創成研究会2014 in ひろしま
  • Place of Presentation: サテライトキャンパス広島（広島県広島市）
  • Year and Date: 2014-08-18 – 2014-08-19
• [Presentation] 金ナノ粒子被覆マイクロビーズを用いた分析法の開発 (2014)
  • Author(s): 木下隆将, 高井善朗, 山本陽二郎, 西野智昭, 椎木 弘, 長岡 勉
  • Organizer: JPCA2014 アカデミックプラザ
  • Place of Presentation: ビッグサイト東京（東京都江東区）
  • Year and Date: 2014-06-04 – 2014-06-06
• [Book] 基礎分析化学 (2015)
  • Author(s): 椎木 弘, 長岡 勉
  • Total Pages: 198
  • Publisher: 朝倉書店
• [Book] 基礎分析化学（第9章 生物学的分析） (2015)
  • Author(s): 椎木 弘, 長岡 勉
  • Total Pages: 208
  • Publisher: 朝倉書店
• [Remarks] 大阪府立大学分子認識化学研究グループHP
  • URL: http://www.chem.osakafu-u.ac.jp/ohka/ohka12/index.html
• [Remarks] 大阪府立大学教員活動情報
  • URL: http://kyoindb.osakafu-u.ac.jp/html/100599_ja.html
• [Remarks] 大阪府立大学教員活動情報
  • URL: http://kyoindb.osakafu-u.ac.jp/html/100599_ja.html?k=%E6%A4%8E%E6%9C%A8%E5%BC%98